Cultured human amniotic fluid cells characterized with antibodies against intermediate filaments in indirect immunofluorescence microscopy.

Virtanen, Ismo; Koskull, Harriet von; Lehto, Veli‐Pekka; Vartio, T; Aula, Pertti

doi:10.1172/jci110382

Cited by 59 publications

(15 citation statements)

References 34 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The subsequent keratinization of the fetal skin, including follicular and interfollicular keratinization, occurs around 22-25 weeks of gestation [49], which would explain why fewer K8 cells are present in the later gestational periods. Although the cells found within the AF have been categorized into three main types: epithelial (E-type), fibroblast-like (F-Type) and amniotic fluids (AFType) cells [51,52], previous studies have demonstrated that keratin staining is restricted to mainly epithelioid (Type-E) and some amniotic fluid (Type-AF) type cells; fibroblast-like (Type-F) cells have been shown to be negative for keratin, suggesting a more mesenchymal origin [55]. Our results certainly support these findings.…”

Section: Early Gestations Of Af Cells Are Of Epithelial Originmentioning

confidence: 99%

Probing Stemness and Neural Commitment in Human Amniotic Fluid Cells

Jezierski

Gruslin

Tremblay

et al. 2010

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Recently, human amniotic fluid (AF) cells have attracted a great deal of attention as an alternative cell source for transplantation and tissue engineering. AF contains a variety of cell types derived from fetal tissues, of which a small percentage is believed to represent stem cell sub-population(s). In contrast to human embryonic stem (ES) cells, AF cells are not subject to extensive legal or ethical considerations; nor are they limited by lineage commitment characteristic of adult stem cells. However, to become therapeutically valuable, better protocols for the isolation of AF stem cell sub-populations need to be developed. This study was designed to examine the molecular components involved in self-renewal, neural commitment and differentiation of AF cells obtained at different gestational ages. Our results showed that, although morphologically heterogeneous, AF cells derived from early gestational periods ubiquitously expressed KERATIN 8 (K8), suggesting that the majority of these cells may have an epithelial origin. In addition, AF cells expressed various components of NOTCH signaling (ligands, receptors and target genes), a pathway involved in stem cell maintenance, determination and differentiation. A sub-population of K8 positive cells (<10%) co-expressed NESTIN, a marker detected in the neuroepithelium, neural stem cells and neural progenitors. Throughout the gestational periods, a much smaller AF cell sub-population (<1%) expressed pluripotency markers, OCT4a, NANOG and SOX2, from which SOX2 positive AF cells could be isolated through single cell cloning. The SOX2 expressing AF clones showed the capacity to give rise to a neuron-like phenotype in culture, expressing neuronal markers such as MAP2, NFL and NSE. Taken together, our findings demonstrated the presence of fetal cells with stem cell characteristics in the amniotic fluid, highlighting the need for further research on their biology and clinical applications.

show abstract

Section: Early Gestations Of Af Cells Are Of Epithelial Originmentioning

confidence: 99%

Probing Stemness and Neural Commitment in Human Amniotic Fluid Cells

Jezierski

Gruslin

Tremblay

et al. 2010

Stem Cell Rev and Rep

View full text Add to dashboard Cite

show abstract

“…Epithelia, such as amniotic membranes, fetal epidermis, and the mucosa of the digestive, respiratory or urogenital tract are potential sources of these cells. They thus provide morphologically and biochemically distinct fetal cells that are isogenic (8). Upon culture some of these cells acquire a morphology resembling that of skin fibroblasts (9).…”

mentioning

confidence: 99%

Specific 1,25-Hydroxycholecalciferol Receptors and Stimulation of 25-Hydroxycholecalciferol- 24R Hydroxylase in Human Amniotic Cells

1987

View full text Add to dashboard Cite

ABSTRACT. We have analyzed the la,25-dihydroxycholecalciferol [1,25(OH)2D3] receptor content of cultured cells from human amniotic fluid. Six cell lines were grown to confluence in a minimum essential medium containing 20% fetal calf serum. All had a normal karyotype, five were male and one was female. Hypertonic cytosol extracts were prepared by sonication followed by centrifugation at Previous reports have shown that rat, murine, and human skin and cultured fibroblasts possessed an effector system for 1,25(OH)2D3 (1-5) suggesting that these tissues could serve as targets for the hormonal form of vitamin D3. Moreover, it has been established that these cells were responsive to 1 ,25(OH)2D3by showing an unequivocal stimulation of 24-OHase (6, 7).

show abstract

“…Therefore, we analyzed collagen XVII expression in amniotic fluid cells, which were cultured for prenatal diagnosis. Previous studies suggested that these cells originated either from urinary bladder or fetal membranes and were mostly epithelial (Virtanen et al, 1981). The presence of collagen XVII mRNA in these cells was confirmed by using RT-PCR amplification and sequencing of the products (Fig.…”

Section: Collagen XVII Is Expressed By Cultured Amniotic Fluid Cellsmentioning

confidence: 59%

Pemphigoid gestationis autoantigen, transmembrane collagen XVII, promotes the migration of cytotrophoblastic cells of placenta and is a structural component of fetal membranes

et al. 2008

View full text Add to dashboard Cite

Cultured human amniotic fluid cells characterized with antibodies against intermediate filaments in indirect immunofluorescence microscopy.

Cited by 59 publications

References 34 publications

Probing Stemness and Neural Commitment in Human Amniotic Fluid Cells

Probing Stemness and Neural Commitment in Human Amniotic Fluid Cells

Specific 1,25-Hydroxycholecalciferol Receptors and Stimulation of 25-Hydroxycholecalciferol- 24R Hydroxylase in Human Amniotic Cells

Pemphigoid gestationis autoantigen, transmembrane collagen XVII, promotes the migration of cytotrophoblastic cells of placenta and is a structural component of fetal membranes

Contact Info

Product

Resources

About