ABSTRACT. We have analyzed the la,25-dihydroxycholecalciferol [1,25(OH)2D3] receptor content of cultured cells from human amniotic fluid. Six cell lines were grown to confluence in a minimum essential medium containing 20% fetal calf serum. All had a normal karyotype, five were male and one was female. Hypertonic cytosol extracts were prepared by sonication followed by centrifugation at Previous reports have shown that rat, murine, and human skin and cultured fibroblasts possessed an effector system for 1,25(OH)2D3 (1-5) suggesting that these tissues could serve as targets for the hormonal form of vitamin D3. Moreover, it has been established that these cells were responsive to 1 ,25(OH)2D3by showing an unequivocal stimulation of 24-OHase (6, 7).

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