Cultured-dependent and cultured-independent study of bacteria associated with Thai commercial stingless bee <i>Lepidotrigona terminata</i>

Suphaphimol, Nattaphon; Attasopa, Korrawat; Pakwan, Chonthicha; Chantawannakul, Panuwan; Disayathanoowat, Terd

doi:10.1080/00218839.2020.1831285

Cited by 10 publications

(12 citation statements)

References 53 publications

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“…are important probiotics (Anderson et al 2011;Vasquez et al 2012), and they can secrete bacteriostatic substances (bacteriocins and lactic acid) to protect their hosts (De Keersmaecker et al 2006;Li et al 2021). Recently, Suphaphimol et al (2020) isolated lactic acid bacteria from the gut of L. terminata that were antagonistic to pathogens of honeybees (Paenibacillus larvae). Therefore, Lactobacillus also plays a key role in the health of stingless bees.…”

Section: Ve Nt Ra Lis T P a G D En I L Ter M In At Amentioning

confidence: 99%

The composition of bacteria in gut and beebread of stingless bees (Apidae: Meliponini) from tropics Yunnan, China

Tang

Miao

Chen

et al. 2021

Antonie van Leeuwenhoek

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Stingless bees are the main pollinators in tropical and subtropical regions. However, there are only a few studies on the structure and composition of bacteria in the gut and beebread of stingless bees, especially in China. To address this shortage of information, we characterized the microbiota of three common species of stingless bees (Lepidotrigona terminata, Lepidotrigona ventralis and Tetragonula pagdeni) and beebread samples of T. pagdeni. The results showed that the gut of stingless bees contained a set of dominant bacteria, including Acetobacter-like, Snodgrassella, Lactobacillus, Psychrobacter, Pseudomonas, Bifidobacterium and other species. The gut microbiota structures of the three stingless bees were different, and the abundances of bacterial species in the gut varied between communities of the same bee species. The reasons for this are manifold and may include food preference, age and genetic differences. In addition, the abundances of Lactobacillus, Carnimonas, Escherichia-Shigella, Acinetobacter and other species were high in the beebread of stingless bees. In conclusion, our findings reveal the bacteria composition and structure of the gut and beebread of stingless bees in China and deepen our understanding of the dominant bacteria of the gut and beebread of stingless bees.

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Section: Ve Nt Ra Lis T P a G D En I L Ter M In At Amentioning

confidence: 99%

The composition of bacteria in gut and beebread of stingless bees (Apidae: Meliponini) from tropics Yunnan, China

Tang

Miao

Chen

et al. 2021

Antonie van Leeuwenhoek

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“…These approaches are generally less laborious but require more efforts on molecular biology and bioinformatics. Culture-independent approaches have been rapidly developed over the past decade due to the applications of high-throughput sequencing technologies such as Next Generation Sequencing (NGS, e.g., 454 pyrosequencing, Illumina sequencing) and long-read Third Generation Sequencing (TGS, e.g., PacBio, Oxford Nanopore [ 38 , 39 , 40 ] Based on genetic and bioinformatic information obtained from culture-independent approaches, we can reasonably characterize the diversity and community composition as well as predict potential interactions and functions of microbes in various environments [ 41 , 42 , 43 ]. However, there are some pitfalls to culture-independent approaches, particularly relating to PCR biases [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Microorganisms Dwelling on the 19th Century Lanna Mural Paintings from Northern Thailand Using Culture-Dependent and -Independent Approaches

Suphaphimol

Suwannarach

Purahong

et al. 2022

Biology

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Lanna painting is a unique type of painting in many temples in the Northern Thai region. Similar to most mural paintings, they usually decay over time partly due to the activity of microbes. This study aimed to investigate the microorganisms from two Lanna masterpiece paintings in two temples that differ in the numbers of visitors using both culture-dependent and -independent approaches. The microorganisms isolated from the murals were also tested for the biodeterioration activities including discoloration, acid production and calcium precipitation. Most microorganisms extracted from the paintings were able to discolor the paints, but only fungi were able to discolor, produce acids and precipitate calcium. The microorganism communities, diversity and functional prediction were also investigated using the culture-independent method. The diversity of microorganisms and functional prediction were different between the two temples. Gammaproteobacteria was the predominant group of bacteria in both temples. However, the fungal communities were different between the two temples as Aspergillus was the most abundant genus in the site with higher number of visitors [Buak Krok Luang temple (BK)]. Conversely, mural paintings at Tha Kham temple (TK) were dominated by the Neodevriesia genera. We noticed that a high number of visitors (Buak Krok Luang) was correlated with microbial contamination from humans while the microbial community at Tha Kham temple had a higher proportion of saprotrophs. These results could be applied to formulate a strategy to mitigate the amount of tourists as well as manage microorganism to slow down the biodeterioration process.

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“…While, Leuconostoc isolates (CMU-LAB 14) were grouped among Leuconostoc previously isolated from another species of stingless bee ( L . terminata ) [ 12 ].…”

Section: Resultsmentioning

confidence: 99%

“…isolated from another stingless bees L . terminata in Thailand [ 12 ]. Therefore, this bacterial functional group might be unique to stingless bee in Thailand.…”

Section: Discussionmentioning

confidence: 99%

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Microbial community profiling and culturing reveal functional groups of bacteria associated with Thai commercial stingless worker bees (Tetragonula pagdeni)

et al. 2023

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Stingless bees play a crucial role in the environment and agriculture as they are effective pollinators. Furthermore, they can produce various products that can be exploited economically, such as propolis and honey. Despite their economic value, the knowledge of microbial community of stingless bees, and their roles on the bees’ health, especially in Thailand, are in its infancy. This study aimed to investigate the composition and the functions of bacterial community associated with Tetragonula pagdeni stingless bees using culture-independent and culture-dependent approaches with emphasis on lactic acid bacteria. The culture-independent results showed that the dominant bacterial phyla were Firmicutes, Proteobacteria and Actinobacteria. The most abundant families were Lactobacillaceae and Halomonadaceae. Functional prediction indicated that the prevalent functions of bacterial communities were chemoheterotrophy and fermentation. In addition, the bacterial community might be able to biosynthesize amino acid and antimicrobial compounds. Further isolation and characterization resulted in isolates that belonged to the dominant taxa of the community and possessed potentially beneficial metabolic activity. This suggested that they are parts of the nutrient acquisition and host defense bacterial functional groups in Thai commercial stingless bees.

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Cultured-dependent and cultured-independent study of bacteria associated with Thai commercial stingless bee Lepidotrigona terminata

Cited by 10 publications

References 53 publications

The composition of bacteria in gut and beebread of stingless bees (Apidae: Meliponini) from tropics Yunnan, China

The composition of bacteria in gut and beebread of stingless bees (Apidae: Meliponini) from tropics Yunnan, China

Identification of Microorganisms Dwelling on the 19th Century Lanna Mural Paintings from Northern Thailand Using Culture-Dependent and -Independent Approaches

Microbial community profiling and culturing reveal functional groups of bacteria associated with Thai commercial stingless worker bees (Tetragonula pagdeni)

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