Cultured Chinese hamster cells undergo apoptosis after exposure to cold but nonfreezing temperatures

Nagle, William A.; Soloff, Bernard L.; Moss, A. J.; Henle, Kurt J.

doi:10.1016/0011-2240(90)90021-u

Cited by 43 publications

(18 citation statements)

References 34 publications

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“…There is increasing evidence, therefore, that apoptosis may represent another manifestation of cold injury, but clearly all cells cannot be as susceptible as those used in the study of Nagle et al [24]; otherwise hypothermic preservation for practical lengths of time would not be possible, which they are. Susceptibility to cold induced apoptosis is likely to depend critically upon the cell growth cycle [24] and the temperature of exposure [25]. Recent studies in cultured hepatocytes and liver endothelial cells have indicated that reactive oxygen species might play a key role in mediating cold induced apoptosis [26].…”

Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 88%

“…For both cell types, apoptosis was not found after exposure to hypothermia in the range +5 to 0 °C, but was induced at lower temperatures, with a maximum response occurring at -10 °C [25]. There is increasing evidence, therefore, that apoptosis may represent another manifestation of cold injury, but clearly all cells cannot be as susceptible as those used in the study of Nagle et al [24]; otherwise hypothermic preservation for practical lengths of time would not be possible, which they are. Susceptibility to cold induced apoptosis is likely to depend critically upon the cell growth cycle [24] and the temperature of exposure [25].…”

Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 97%

“…While many of the diverse stresses known to cause necrotic cell death have also been reported to induce apoptosis in a variety of cells, the role of low temperatures as a possible stimulus of programmed cell death has only recently begun to emerge [23]. The earliest reports appeared in 1990 when it was shown that cultured mammalian fibroblast cells at the transition from logarithmic to stationary growth were killed by brief exposures to 0 °C and rewarming to 37 °C [24]. The observed cell killing required only a few minutes of hypothermic exposure, and the affected cells exhibited characteristics of apoptosis.…”

Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 99%

“…The observed cell killing required only a few minutes of hypothermic exposure, and the affected cells exhibited characteristics of apoptosis. Moreover, the kinetics of cell death served to distinguish cold induced apoptosis from the lethal effects of longer-term cold storage (hours or day), and also showed characteristics inconsistent with direct chilling injury (cold shock) [24]. More recently, we have reported that apoptosis can be detected in a significant proportion of both vascular smooth muscle and en- dothelial cells subjected to brief (30-120 s) cooling [25].…”

Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 99%

“…Recent studies in cultured hepatocytes and liver endothelial cells have indicated that reactive oxygen species might play a key role in mediating cold induced apoptosis [26]. It is becoming increasingly more evident that apoptosis plays an integral role in cell death induced by the rigors of both hypothermia and cryopreservation [23,24,[27][28][29]. More specifically, apoptosis has been identified to be directly associated with delayed onset cell death (DOCD).…”

Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 99%

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Hypothermic Blood Substitution: Special Considerations for Protection of Cells during ex vivo and in vivo Preservation

Taylor¹

2007

Transfus Med Hemother

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Clinical applications of hypothermia range from ex vivo storage of cells, tissues and organs for transplantation or cell-based therapies to in vivo protection of a patient during complex surgeries that might entail circulatory and/or cardiac arrest. The intent of this brief review is to outline the essential principles of hypothermia applied in the preservation of cells for a multitude of clinical purposes. The focus will be confined to our ability as interventionalists to control the environment of cells to optimize hypothermic preservation. More specifically, this review will summarize the design principles of hypothermic blood substitutes (HBS) defined as preservation solutions for protection of cells, tissues and organs during a hypothermic preservation interval. In contrast to the types of solutions that have been used historically as hemodiluents for clinical hypothermia, i.e. normal physiologically balanced salt solutions, our approach has been to design aqueous blood substitutes that embody many of the principles now identified as contributory and important for optimal ex vivo organ preservation. The working hypothesis that we have addressed using a variety of pre-clinical large animal models is that acellular solutions can be designed to act as universal tissue preservation solutions during several hours of hypothermic whole-body washout involving cardiac arrest, with or without circulatory arrest. On this basis, two new types of solutions, designated ‘Purge’ and ‘Maintenance’ HBS, have been formulated and evaluated to fulfill separate requirements during the asanguineous procedure. The evidence supporting this concept is reviewed in the context of imminent clinical trials to apply this novel technique for the preservation and resuscitation of trauma victims suffering hemorrhagic shock.

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Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 88%

Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 97%

Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 99%

Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 99%

Section: Apoptosis Vs Necrosis In Cold Induced Cell Deathmentioning

confidence: 99%

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Hypothermic Blood Substitution: Special Considerations for Protection of Cells during ex vivo and in vivo Preservation

Taylor¹

2007

Transfus Med Hemother

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Two-dimensional manipulation of differentiated Madin-Darby canine kidney (MDCK) cell sheets: The noninvasive harvest from temperature-responsive culture dishes and transfer to other surfaces

Kushida

Yamato

Kikuchi

et al. 2000

J. Biomed. Mater. Res.

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A renal epithelial cell line, Madin-Darby canine kidney (MDCK) cells, adheres, spreads, and proliferates to confluency on our developed temperature-responsive culture dishes grafted with a poly(N-isopropylacrylamide) (PIPAAm) at 37 degrees C. In addition to other cell types, including hepatocytes and endothelial cells, MDCK cell sheets noninvasively were harvested from PIPAAm-grafted dishes merely by reducing the temperature. However, during the early stage of culture (up to 3 weeks), confluent MDCK cell detachment is greatly repressed. In the present study, we succeeded in the rapid harvest of confluent MDCK cell sheets and intact transfer to other culture dishes by utilizing hydrophilically modified poly(vinylidene difluoride) (PVDF) membranes as supporting materials. Immunocytochemistry with anti-beta-catenin antibody revealed that the functional cell-cell junctions were well organized in the transferred MDCK cell sheets. The viability assay showed that the transferred cells were not damaged during the two-dimensional cell-sheet manipulation. By transmission electron microscopy it was confirmed that the harvested MDCK cells retained differentiated phenotypes and had many microvilli and tight junctions at the apical and lateral plasma membranes, respectively. This two-dimensional cell-sheet manipulation technique promises to be useful in tissue engineering as well as in the investigation of epithelial cell sheets.

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Hypothermia-Enhanced Human Tumor Cell Radiosensitivity

1997

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Cultured Chinese hamster cells undergo apoptosis after exposure to cold but nonfreezing temperatures

Cited by 43 publications

References 34 publications

Hypothermic Blood Substitution: Special Considerations for Protection of Cells during ex vivo and in vivo Preservation

Hypothermic Blood Substitution: Special Considerations for Protection of Cells during ex vivo and in vivo Preservation

Two-dimensional manipulation of differentiated Madin-Darby canine kidney (MDCK) cell sheets: The noninvasive harvest from temperature-responsive culture dishes and transfer to other surfaces

Hypothermia-Enhanced Human Tumor Cell Radiosensitivity

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