Culture of triploid tissue from the endosperm of an endangered Chilean tree species<i>Gomortega keule</i>

Muñoz-Concha, Diego

doi:10.1080/14620316.2015.1110995

Cited by 7 publications

(2 citation statements)

References 15 publications

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“…The appearance of regenerants with a DNA content similar to the mother plant (hexaploid) might be due to the introduction of some mother plant or embryo cells as contaminants in the endosperm during isolation from the seed. This has been noted also, for example, during chromosome counting in cultured cell aggregates derived from endosperm tissue of Gomortega keule , where some cells were found to possess a diploid number of chromosomes [ 32 ].…”

Section: Discussionmentioning

confidence: 91%

Sex-Linked Molecular Markers Identify Female Lines in Endosperm-Derived Kiwifruit Callus and in Regenerants

Chłosta

Kwolek

Śliwińska

et al. 2021

Plants

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This is the first report of molecular markers application for the analysis of endosperm-derived callus and nonaploid kiwifruit (Actinidia chinensis var. deliciosa, formerly: Actinidia deliciosa) plants. As a source of explants, fruits of ‘Hayward’, the most popular cultivar, were used. Additionally, analyses of the nuclear DNA content and sex were conducted on the regenerated plants. Hexaploid seedlings were used as control for the flow cytometric analyses. Most of the plants (about 90%) regenerated via endosperm-derived callus possessed 2C = 9Cx DNA, which confirmed their endosperm origin and nonaploidy. Because Actinidia is a dioecious species, and female plants bearing fruits are desired by breeders, it is crucial to identify the sex of an individual at early stages of development. Analyses were conducted with ex vitro and in vitro samples. Results revealed that specific markers for a Y-chromosome applied at the callus stage allowed us to reliably predict the sex of plants regenerated from it. This is a novel application of sex-linked markers for early selection of female and male callus lines when the sex of the initial explants is still unknown, such as fresh isolated embryos and endosperm. It may have significant importance for breeding kiwifruit programs, which involve tissue culture techniques.

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Section: Discussionmentioning

confidence: 91%

Sex-Linked Molecular Markers Identify Female Lines in Endosperm-Derived Kiwifruit Callus and in Regenerants

Chłosta

Kwolek

Śliwińska

et al. 2021

Plants

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“…Furthermore, the plant growth regulators (PGRs) added to the culture medium, the sources of the explant material, and the cultivar of the donor plants all play an important role in establishing a successful regeneration protocol from endosperm explants (Wang et al 2016). Despite being challenging task, the recently published protocols for endosperm-derived plant regeneration in Gomortega keule (Muñoz-Concha 2016), Melia azedazach (Thang et al 2018), Passiflora edulis (Antoniazzi et al 2018), and Passiflora cincinnata (Silva et al 2020) indicate that this technique is still attracting interest and is worth applying to economically important plant species.…”

Section: Introductionmentioning

confidence: 99%

Effect of medium composition, genotype and age of explant on the regeneration of hexaploid plants from endosperm culture of tetraploid kiwiberry (Actinidia arguta)

Abdullah

Śliwińska

Góralski

et al. 2021

Plant Cell Tiss Organ Cult

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Endosperm, an ephemeral and storage tissue, serves as a source of nutrition and protection during embryo development and germination. It can be used for the cultivation of polyploid plants in vitro. Here, results of plant regeneration and acclimatization from the endosperm-derived calli of four cultivars of Actinidia arguta has been presented. Seeds excised from fresh fruit and dry seeds stored for one year served as the sources of endosperm explants of selected tetraploid cultivars of A. arguta. Callus Induction Medium (CIM; containing 0.25, 0.5, or 1 mg/l of TDZ) and Actinidia Endosperm Medium (AEM; containing 2 mg/l of 2,4-D and 5 mg/l of kinetin) were used to study the organogenic responses of the calli. On AEM, the source of explant did not significantly affect the rate of callus induction for any of the tested cultivars; no organogenic events were observed. In contrast, on CIM both the source of explants and the cultivar origin caused significant differences in callus formation and subsequent organogenic events. Histological and ultrastructural analyses revealed the adventitious nature of shoot bud formation on these media. The most efficient elongation of shoot buds was achieved after transferring organogenic calli with adventitious shoot buds to a medium supplemented with zeatin or meta-topolin. Robust root induction with minimal basal callus formation occurred on the medium with indole-3-acetic acid. Flow cytometric analysis revealed that the nuclear DNA content in the leaves of some regenerants was approximately 50 % higher (4.5 pg/2C) than that in leaves from the tetraploid seedlings (3.1 pg/2C),which confirmed that those regenerants originated from the endosperm. The regeneration of such hexaploid plants was more efficient when endosperm from fresh seeds served as an explant; therefore, fresh rather than dry seeds are recommended for endosperm-derived plant production. The hexaploid plants of A. arguta can serve as an important source of breeding material.

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Somatic Embryogenesis in Gomortega keule

Muñoz-Concha

2018

Step Wise Protocols for Somatic Embryogenesis of Important Woody Plants

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Culture of triploid tissue from the endosperm of an endangered Chilean tree speciesGomortega keule

Cited by 7 publications

References 15 publications

Sex-Linked Molecular Markers Identify Female Lines in Endosperm-Derived Kiwifruit Callus and in Regenerants

Sex-Linked Molecular Markers Identify Female Lines in Endosperm-Derived Kiwifruit Callus and in Regenerants

Effect of medium composition, genotype and age of explant on the regeneration of hexaploid plants from endosperm culture of tetraploid kiwiberry (Actinidia arguta)

Somatic Embryogenesis in Gomortega keule

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