Culture of mature trophoblastic giant cells from bovine placentomes

Landim, L. P.; Miglino, M. A.; Pfarrer, Christiane; Ambrósio, Carlos Eduardo; Garcia, Julia

doi:10.1016/j.anireprosci.2006.04.053

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…The proportion of binucleate trophoblast giant cells among other cotyledonary cells was moderately higher than that found by others (Landim et al, 2007;Vanselow et al, 2008), showing a relative proportion of about 30-40% after percoll gradient centrifugation and ablation of fibroblast cells. Perhaps this might be due to different isolation periods of cotyledonary and different time of disaggregation.…”

Section: Discussioncontrasting

confidence: 59%

“…Since AP-2 proteins have a featured helix-span-helix motif at the carboxyl terminus and a central basic region, which can form homo-or hetero-dimeric complexes with some specific novel group of coactivators to bind to a particular promoter (Eckert et al, 2005), by this way they can modulate transactivation capability or DNA-binding affinity of the other AP-2 family members, thus further experiments are required to identify the specific properties of each complex when the CSH1, PRP1 and PAG1 gene are in the inactive and active states. In addition, AP-2 proteins can be viewed as gatekeepers controlling the balance between proliferation and differentiation during embryogenesis (Hilger-Eversheim et al, 2000;Landim et al, 2007), therefore, the signaling pathways responsible for induction of AP-2 genes are currently under investigation, these information will provide a better understanding of placentation and embryogenesis.…”

Section: Discussionmentioning

confidence: 99%

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Delivery of AP-2α siRNA into cultured bovine trophoblast cells by electroporation repressed key placenta-specific gene expression

Zhou

Wang

Zhang

et al. 2012

Gene

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Section: Discussioncontrasting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Delivery of AP-2α siRNA into cultured bovine trophoblast cells by electroporation repressed key placenta-specific gene expression

Zhou

Wang

Zhang

et al. 2012

Gene

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“…27,28 We used serum deprivation and low oxygen tension as 2 sources of stress since each is known to induce enhanced apoptosis in trophoblasts. [29][30][31][32] Trophoblasts cultured in vehicle control or ET-1 were exposed to serum-free medium in 20% oxygen for a total of 16 hours, or 24 hours prior to harvest at 72 hours, or to an environment with an oxygen concentration of 8%, which resembles villous oxygen tension in vivo after the first trimester 33 or <1% oxygen, in addition to standard 20% oxygen. The medium was equilibrated with each gas mixture 30 minutes prior to use.…”

Section: Trophoblast Isolation and Culturementioning

confidence: 99%

Endothelin-1 Attenuates Apoptosis in Cultured Trophoblasts From Term Human Placentas

Cervar-Zivkovic

Barton

et al. 2007

Reprod. Sci.

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The authors test the hypothesis that endothelin-1 (ET-1) modulates apoptosis in human term trophoblasts. Primary cultures of cytotrophoblasts from term human placentas (n = 5) were cultured for 16 hours total or 24 hours prior to harvest at 72 hours in atmospheres of <1%, 8%, and 20% oxygen, in the presence of 10% serum, ET-1 (1-100 pmol/mL), both, or neither. The apoptotic cleavage products of poly-ADP-ribose polymerase and cytokeratin 18 filaments were quantified by Western analysis and immunocytochemistry. The expression of BAD, pBAD-serine 112, p53, and 2 isoforms of MDM2 were quantified by immunoblotting, and endothelin A and B receptors were analyzed by immunocytochemistry. Compared to vehicle control, increasing concentrations of ET-1 reduce by 3- to 6-fold the level of apoptosis in cytotrophoblasts exposed to serum-free conditions at 20% oxygen. Similarly, syncytiotrophoblast cultures grown for 24 hours without serum in 100 pmol/mL ET-1 show a 3-fold lower level of apoptosis compared with vehicle control. ET-1 significantly reduces apoptosis in cultures exposed to 20% oxygen but not in cultures exposed to 8% or 1% oxygen. The effect of ET-1 on apoptosis in 20% oxygen is accompanied by reduced p53 expression and is correlated with enhanced expression of endothelin B receptor, compared to cultures in 8% or 1% oxygen. ET-1 reduces apoptosis in cultured human trophoblasts, and this finding suggests a role for ET-1 in protecting trophoblasts against injury.

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“…Com passar dos anos e desenvolvimento de novas tecnologias, pesquisadores conseguiram estabelecer um protocolo efetivo para cultivo de CGT bovina pelo período de 90 dias. Durante este período as CGTB eram isoladas das demais CGT onde permaneciam vivas até mesmo na presença do substrato colágeno (LANDIM JR. et al, 2007). Este achado pode ser explicado pelo fato da cultura utilizada ser composta de 90% de CGT e 10% de fibroblastos.…”

Section: Conexina 43unclassified

Expressão das conexinas 32 e 43 em células trofoblásticas da placenta bovina em cultura celular

Birck¹

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Lílian de Jesus Oliveira, a vida sempre nos reserva coisas boas, obrigado por tudo. Ao Diogo pela amizade, convivência e momentos de risos no laboratório, valeu. Fernanda Agreste, pela amizade. Gisele Saviani pela sensibilidade e ajuda nas horas que precisei pude contar com você, ficai registrado aqui neste trabalho a nossa amizade, muito obrigado. Aos professores e servidores técnico administrativo do setor de Anatomia dos Animais Domésticos e Silvestres da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, por me acolherem em seu convívio. Ao frigorífico Mantiqueira, localizado na cidade de São José dos Campos, ao pessoal da linha de abate, aos Veterinários do SIF, e Diretores do frigorífico por cederem suas dependências e material para realização de nosso estudo. UFPR Universidade Federal do Paraná Campus Palotina, esta universidade que me acolheu por 14 anos e que me deu oportunidade de crescer como ser humano e intelectualmente e, aos grandes amigos que lá deixei, meu muito obrigado. Universidade Federal de Lavras-UFLA, minha nova casa onde pretendo demonstrar meu conhecimento teórico e prático adquirido através de muitas horas de dissecação e pesquisa, obrigado por me acolherem de portas abertas. Aos técnicos e secretários do curso de pós-graduação da Faculdade de Medicina Veterinária e Zootecnia da USP, pelos anos de amizade e convívio. A Henrique Ribeiro Alves de Resende, ex-colega de pós-graduação, amigo e hoje colega de trabalho, quem diria, mas a vida nos reserva sempre muitas surpresas, a você também meu mestre, a quem me espelho, meu muito obrigado. A Luciano da Silva Alonso ex-colega também, amigo sempre disposto a ajudar e a contar muitos casos hilários, um abraço. A André Luís Filadelpho pela amizade construída desde o mestrado e que permanece até hoje não importando a distância.

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Culture of mature trophoblastic giant cells from bovine placentomes

Cited by 8 publications

References 26 publications

Delivery of AP-2α siRNA into cultured bovine trophoblast cells by electroporation repressed key placenta-specific gene expression

Delivery of AP-2α siRNA into cultured bovine trophoblast cells by electroporation repressed key placenta-specific gene expression

Endothelin-1 Attenuates Apoptosis in Cultured Trophoblasts From Term Human Placentas

Expressão das conexinas 32 e 43 em células trofoblásticas da placenta bovina em cultura celular

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