Culture of immobilized insect cells

Archambault, Jean; Robert, J.; Tom, Lisa

doi:10.1007/bf00369629

Cited by 11 publications

(3 citation statements)

References 19 publications

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“…Overall, the cells grew 38-and 32-fold on BioNOC and BioNOC II, respectively (Table 1) and the cell yields were high compared to those using other immobilizing carriers (Kompier et al 1991, Archambault et al 1994. All these data further validated their use in BelloCell.…”

Section: Effects Of Carrierssupporting

confidence: 67%

“…Once the cells resumed growing, the cells exhibited similar behavior in all cultures. Among these five inoculum sizes, 3 · 10 7 cells resulted in 142-fold increase in the final cell yield, which was higher than those achieved in other carrier types and reactors (Reiter et al 1991, Archambault et al 1994. The drawback was that the lag phase was lengthy.…”

Section: Effects Of Inoculum Sizecontrasting

confidence: 50%

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A Novel Oscillating Bioreactor BelloCell: Implications for Insect Cell Culture and Recombinant Protein Production

Chung

Chan

et al. 2005

Biotechnol Lett

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BelloCell is a novel packed bed bioreactor that allows alternating nutrient and gas transfer to a culture. Spodoptera frugiperda Sf-9 grown in the BelloCell (300 ml culture) reached 1.3-1.5x10(7) cells ml-1 in 7-8 days and the total baculovirus-expressed protein yield was 2.3-times that in a stirred tank bioreactor (600 ml culture). The superior cell and protein yields underline the potential of BelloCell for cell culture and recombinant protein production.

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Section: Effects Of Carrierssupporting

confidence: 67%

Section: Effects Of Inoculum Sizecontrasting

confidence: 50%

A Novel Oscillating Bioreactor BelloCell: Implications for Insect Cell Culture and Recombinant Protein Production

Chung

Chan

et al. 2005

Biotechnol Lett

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“…This value is inferior to our own maximal attained densities of 4 × 10 8 (8 × 10 6 cells cm -2 ) and 2.9 × 10 8 cells g -1 (5.8 × 10 6 cells cm -2 ) for Sf-9 and High-Five cells, respectively (Figure 2, taking into account that 1 g of Fibra-Cel has 50 cm 2 of projected area). Similarly, these maximal densities are much higher than that obtained from Sf-9 cells immobilized on sheets of nonwoven polyester fibers in a 2-L bioreactor (2.47 × 10 6 cells cm -2 of gross surface area or 1.23 × 10 6 cells cm -2 of projected area) (39).…”

Section: Discussionmentioning

confidence: 77%

Microcarrier Culture of Lepidopteran Cell Lines: Implications for Growth and Recombinant Protein Production

Ikonomou¹,

Drugmand²,

Bastin

et al. 2002

Biotechnology Progress

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Several microcarrier systems were screened with Sf‐9 and High‐Five cell lines as to their ability to support cell growth and recombinant (β‐galactosidase) protein production. Growth of both cell lines on compact microcarriers, such as Cytodex‐1 and glass beads, was minimal, as cells detached easily from the microcarrier surface and grew as single cells in the medium. Cell growth was also problematic on Cytopore‐1 and ‐2 porous microcarriers. Cells remained attached for several days inside the microcarrier pores, but no cell division and proliferation were observed. On the contrary, insect cells grew well in the interior of Fibra‐Cel disks mainly as aggregates at points of fiber intersection, reaching final (plateau) densities of about 4 × 106 (Sf‐9) and 2.7 × 106 (High‐Five) cells mL‐1 (8 × 106 and 5.5 × 106 cells per cm2 of projected disk area, respectively). Their growth was described well by the logistic equation, which takes into account possible inhibition effects. β‐Galactosidase (β‐gal) production of Sf‐9 cells on Fibra‐Cel disks (infected at 3.3 × 106 cells mL‐1) was prolonged (192 h), and specific protein production was similar to that of high‐density free cell infection. Cultispher‐S microcarriers were found to be a very efficient system for the growth of High‐Five cells, whereas no growth of Sf‐9 cells took place for the same system. Concentrations of about 9 × 106 cells mL‐1 were reached within 120 h, with cell growth in both microcarriers and aggregates, appearance of cellular bridges between microcarriers and aggregates, and eventual formation of macroaggregates incorporating several microcarriers. Specific protein productions after β‐gal baculovirus infection at increasing cell concentrations were almost constant, thus leading to elevated volumetric protein production: final β‐gal titers of 946, 1728, and 1484 U mL‐1 were obtained for infection densities of 3.4, 7.2, and 8.9 × 106 cells mL‐1, respectively.

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Insect cell bioreactors

Agathos¹

1996

Insect Cell Culture: Fundamental and Applied Aspects

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Culture of immobilized insect cells

Cited by 11 publications

References 19 publications

A Novel Oscillating Bioreactor BelloCell: Implications for Insect Cell Culture and Recombinant Protein Production

A Novel Oscillating Bioreactor BelloCell: Implications for Insect Cell Culture and Recombinant Protein Production

Microcarrier Culture of Lepidopteran Cell Lines: Implications for Growth and Recombinant Protein Production

Insect cell bioreactors

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