Insect cell bioreactors

Agathos, Spiros N.

doi:10.1007/0-306-46850-6_15

Cited by 4 publications

(4 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, in other reports no direct effects of cell density levels or of nutrient replacement strategies on productivity have been observed (20,21). It was suggested that this may indicate the presence of other simultaneous influences that should be examined for their role on infectivity and production at key points in the development of the population (22). Early results proposed that the variation in product yield could be a result of the cell cycle phase of individual cells at the time of infection (23).…”

Section: Introductionmentioning

confidence: 96%

Cell Cycle Progression in Serum-Free Cultures of Sf9 Insect Cells: Modulation by Conditioned Medium Factors and Implications for Proliferation and Productivity

et al. 2000

View full text Add to dashboard Cite

Cell cycle progression was studied in serum-free batch cultures of Spodoptera frugiperda (Sf9) insect cells, and the implications for proliferation and productivity were investigated. Cell cycle dynamics in KBM10 serum-free medium was characterized by an accumulation of 50-70% of the cells in the G(2)/M phase of the cell cycle during the first 24 h after inoculation. Following the cell cycle arrest, the cell population was redistributed into G(1) and in particular into the S phase. Maximum rate of proliferation (micro(N, max)) was reached 24-48 h after the release from cell cycle arrest, coinciding with a minimum distribution of cells in the G(2)/M phase. The following declining micro(N) could be explained by a slow increase in the G(2)/M cell population. However, at approximately 100 h, an abrupt increase in the amount of G(2)/M cells occurred. This switch occurred at about the same time point and cell density, irrespective of medium composition and maximum cell density. An octaploid population evolved from G(2)/M arrested cells, showing the occurrence of endoreplication in this cell line. In addition, conditioned medium factor(s) were found to increase micro(N,max), decrease the time to reach micro(N,max), and decrease the synchronization of cells in G(2)/M during the lag and growth phase. A conditioned medium factor appears to be a small peptide. On basis of these results we suggest that the observed cell cycle dynamics is the result of autoregulatory events occurring at key points during the course of a culture, and that entry into mitosis is the target for regulation. Infecting the Sf9 cells with recombinant baculovirus resulted in a linear increase in volumetric productivity of beta-galactosidase up to 68-75 h of culture. Beyond this point almost no product was formed. Medium renewal at the time of infection could only partly restore the lost hypertrophy and product yield of cultures infected after the transition point. The critical time of infection correlated to the time when the mean population cell volume had attained a minimum, and this occurred 24 h before the switch into the G(2)/M phase. We suggest that the cell density dependent decrease in productivity ultimately depends on the autoregulatory events leading to G(2)/M cell cycle arrest.

show abstract

Section: Introductionmentioning

confidence: 96%

Cell Cycle Progression in Serum-Free Cultures of Sf9 Insect Cells: Modulation by Conditioned Medium Factors and Implications for Proliferation and Productivity

et al. 2000

View full text Add to dashboard Cite

show abstract

“…The fact that the virus replication cycle is lytic means that protein production is not continuous, requiring infection of new cultures frequently in fed-batch or semi-continuous bioreactor systems (van Lier et al, 1990;Tramper et al, 1993;Agathos, 1996). In contrast to the pathogenic baculoviruses, polydnaviruses have a symbiotic relationship with their insect hosts, parasitic hymenopteran wasps.…”

Section: Expression At the Optimal Timementioning

confidence: 98%

Opportunities and challenges for the baculovirus expression system

Oers

2011

Journal of Invertebrate Pathology

132

View full text Add to dashboard Cite

“…Because the virus is lytic, attention has also to be paid when deciding for optimal MOI and very often the use of a semicontinuous system or repeated batches was preferred over continuous production. During the course of cell culture, an inevitable loss of production happens, probably due to a high proportion of defective viral particles (Agathos 1996). Despite the variety of bioreactor designs that have been studied, no single system has proven to be universally superior in productivity and ease of scale-up.…”

Section: Improving Receptor Expression and Purification For Large Scamentioning

confidence: 99%

New tools for G-protein coupled receptor (GPCR) drug discovery: combination of baculoviral expression system and solid state NMR

Ratnala

2006

Biotechnol Lett

View full text Add to dashboard Cite

Biotechnology using molecular biology, biochemistry, biophysics, and computational approaches provides an alternative approach for classical pharmacological screening to look at ligand-receptor interactions and receptor specificity, which should support the design of selective drugs based on detailed structural principles. This review addresses specific approaches to study function, structure and relevance of a major pharmaceutical target, namely the G-Protein Coupled Receptors (GPCRs). The main aim of this review has been to exploit and combine GPCR over-expression in a baculoviral expression system with solid-state MAS NMR (ssNMR) approaches for the elucidation of electronic structures of the coordinating ligands/drugs and their modes of interactions with the GPCRs. This review summarizes the approaches, possible future experiments and developments using the above combination of tools for GPCR drug discovery.

show abstract

Insect cell bioreactors

Cited by 4 publications

References 62 publications

Cell Cycle Progression in Serum-Free Cultures of Sf9 Insect Cells: Modulation by Conditioned Medium Factors and Implications for Proliferation and Productivity

Cell Cycle Progression in Serum-Free Cultures of Sf9 Insect Cells: Modulation by Conditioned Medium Factors and Implications for Proliferation and Productivity

Opportunities and challenges for the baculovirus expression system

New tools for G-protein coupled receptor (GPCR) drug discovery: combination of baculoviral expression system and solid state NMR

Contact Info

Product

Resources

About