Culture of human umbilical vein endothelial cells on immobilized vascular endothelial growth factor

Abstract

Vascular endothelial growth factor (VEGF) was immobilized on substrata in photoreactive gelatin to control the adhesion and growth of vascular endothelial cells. The gelatin and VEGF were mixed in water and cast on a polystyrene dish or a silane-coated glass plate. The surface was then photoirradiated in the presence or absence of a photomask and washed. Toughness of the immobilized material was confirmed by ethanol treatment. Human umbilical vein endothelial cells (HUVECs) grew on the immobilized VEGF but not on a nontreated surface. Growth of HUVEC increased significantly with an increase in the amount of immobilized VEGF, and the effects were inhibited by treatment with anti-VEGF antibody. Thus, immobilized VEGF specifically interacted with HUVECs to permit growth in culture. Micropatterning of HUVEC cultures was also achieved using micropattern-immobilized VEGF. This patterning technique may be useful for the formation of blood vessel networks in vitro.

Persistent elevation of plasma vascular endothelial growth factor levels during the first month after minimally invasive colorectal resection

“…To evaluate the effect of VEGF in vitro, endothelial rather than tumor cell cultures are used. In endothelial cell cultures, findings have clearly shown that increasing the VEGF concentration increases the rate of endothelial cell proliferation and invasion [42,43].…”

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

“…121 Patterning of HUVECs has also been achieved with micropatterned-immobilized VEGF. 122 Through micropatterning, EC and EPC adhesion, elongation, and growth can be more precisely controlled. Optimization of micropatterns can lead to a geometrically, chemically, and mechanically functional graft that better replicates native blood vessel architecture and biological cues for endothelialization.…”

Covalent Growth Factor Immobilization Strategies for Tissue Repair and Regeneration

“…Dermal wound healing Polystyrene [18,22,23] PCL/PCL-PEG [14] PCL/gelatin [13] General mitogen Polystyrene [25] PMMA [99] Hepatocyte culture Glass [28,100] Cartilage repair Chitosan [64] Stem cell differentiation (neural) SAMs [90] Polystyrene [21] Stem cell differentiation (osteoblast) PMMA-g-PEG [91] Stem cell survival PMMA-g-PEG [59] Corneal repair PDMS [101,102] IGF-1 Myogenic differentiation Silicone [103] Dermal wound healing Polystyrene [22] Pancreatic stem cell differentiation Glass [62] LIF Maintenance of ESC pluripotency POMA [29] PET [104] Gelatin [88] NGF Stem cell differentiation (neural) PCL/PCL-PEG [17] Neurite extension Glass [61] pHEMA [54] Polypyrrole [26] PDGF-AA Stem cell differentiation (neural) Agarose [55] PDGF-BB Angiogenesis/Vascularization PEG [35] Demineralized bone [41] VEGF Angiogenesis/Vascularization PEG [19,33] Collagen [12,40,56] SAMs [27] PLGA [4] Gelatin [105] Stem cell differentiation (vascular/blood) Chitosan/collagen IV [10] Titanium [52] Agarose [39] a) Polymer abbreviations: PCL, poly(caprolactone); PDMS, poly(dimethyl siloxane); PEG, poly(ethylene glycol); PET, poly(ethylene terephthalate); pHEMA, poly(hydroxyethyl m...…”