Culture of canine synoviocytes on porcine intestinal submucosa scaffolds as a strategy for meniscal tissue engineering for treatment of meniscal injury in dogs

Warnock, Jennifer J.; Spina, Jason; Bobe, Gerd; Duesterdieck-Zellmer, Katja F.; Ott, Jesse; Baltzer, Wendy I.; Bay, Brian K.

doi:10.1016/j.tvjl.2013.10.038

Cited by 8 publications

(6 citation statements)

References 51 publications

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“…Some parts of the cartilage on the femoral and tibial articular surface taken from the knee joints of each group were separately lyophilized to obtain the dry weight. Then, the piece was digested and used for glycosaminoglycan (GAG) and collagen (COL) analyses [ 19 , 20 ]. The concentration of GAG was determined through the di-methyl-methylene blue sulfated GAG assay using a UV/VIS spectrophotometer (Optizen Pop, Mecasys, Daejeon, Korea).…”

Section: Methodsmentioning

confidence: 99%

Adipose-derived mesenchymal stem cells and platelet-rich plasma synergistically ameliorate the surgical-induced osteoarthritis in Beagle dogs

2016

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BackgroundThe purpose of this study is to investigate the clinical effects of platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cell (MSC) as the fundamental treatment of osteoarthritis (OA).MethodsTwenty four Beagle dogs were used as cranial cruciate ligament transection models. The dogs were divided into four groups (n = 6) according to the intra-articular injection materials: the control group with phosphate-buffered saline (PBS), the PRP group with PRP, the MSC group with MSCs emerged in PBS, and the MSC and PRP co-treatment (MP) group with MSCs and PRP.ResultsLameness score, focal compression strength, articular extracellular matrix (ECM) compositions, histopathology, and real-time PCR were used to evaluate the effects of PRP and MSCs on canine OA. In the order of MP, PRP, and MSC group, these all showed positive effects on the evaluated categories. The lameness scores were lower, and the focal compression strengths of the affected femoral articular surface cartilages were higher than those in the OA control group. Also, the inflammatory changes, when evaluated with Mankin scoring and histomorphologic examination, were significantly ameliorated with the treatment of PRP and/or MSCs. The glycosaminoglycan and collagen composition of extracellular matrix was more favorable in the test groups. The ECM-related genes significantly increased through the up-regulation, while the protein expressions of inflammatory cytokines were decreased through the inhibitory effects of PRP and MSCs on chondrocyte apoptosis and inflammatory cytokines.ConclusionsTaken together, this study suggests that PRP and MSCs treatments have a beneficial effect on OA via the stimulation of ECM synthesis and chondrocyte proliferation and via the inhibition of inflammatory reaction.Electronic supplementary materialThe online version of this article (doi:10.1186/s13018-016-0342-9) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Adipose-derived mesenchymal stem cells and platelet-rich plasma synergistically ameliorate the surgical-induced osteoarthritis in Beagle dogs

2016

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“…IL-6 can be produced by four main cell types of the synovial membrane [21,42,44,45]. In our study none of the factors used, either individually or in combination, changed the expression of the IL-6 gene.…”

Section: Discussionmentioning

confidence: 46%

“…Collagen type I within synovial membrane is produced by synovial fibroblasts [21]. Both IGF1 [22] and TGFb1 [23] enhance collagen type I gene expression in different cell types such as smooth muscle cells [22] or fibroblasts, and Ito cells [23].…”

Section: Discussionmentioning

confidence: 99%

Influence of IGF1, TGFβ1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells

Osiecka-Iwan

Moskalewski

Hyc

2016

Folia Histochem Cytobiol.

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The response of the studied genes represents a resultant activity of all major cell types building the synovial membrane, i.e. highly specialized synovial fibroblasts, macrophages, epithelial cells and adipocytes. The results of our study can improve understanding of synovial membrane responses to the intraarticular injections of platelet-rich plasma (PRP) used for the treatment of joint ailments, since PRP contains factors which are also present in CIF.

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“…SOX9 is a transcription factor essential for chondrogenic differentiation 62) , and aggrecan and collagen is a key component of the ECM of healthy cartilage 39) . Prevention of these ECM degradations, therefore, has been treated as a valuable anti-OA target for various treatments 38,39,63) . In the present study, significant decreases of the ECM related chondrogenic mRNA expressionscollagen type II, aggrecan and SOX9 mRNA expressions were demonstrated in the femur and tibia AC OA control as compared with sham control rats.…”

Section: Discussionmentioning

confidence: 99%

Selection of the optimal herbal composition of pomegranate concentrated powder from aqueous extracts of and to treat osteoarthritis in rats

Choi¹,

Kang

et al. 2017

J Korean Med

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We investigated whether a mixture of the main component of pomegranate concentrated powder (PCP) with appropriate proportions of Eucommiae Cortex (EC) and Achyranthis Radix (AR) could act synergistically as an effective treatment for osteoarthritis (OA). Methods: In order to evaluate the effects of PCP, EC, and AR against OA, knee thicknesses, maximum extension angle of each knee, antiinflammation effects, the transcript levels of chondrogenic genes mRNA expressions in femur and tibia articular cartilage (AC) with synovial membrane (SM) were analyzed. In addition, the histopathology and immunohistochemistry of the femur and tibia AC or SM were performed. Results: The surgically-induced OA signs in rats were significantly inhibited by 28 days of continuous treatment of PCP, EC and AR single formulas, and PCP with EC:AR mixed formulas. Especially, PCP with EC:AR 4:1, 2:1 and 1:1 mixed formula treatment constantly showed significantly more favorable inhibitory activities, as compared with those of single formula of PCP, EC and AR treated rats. Conclusion: PCP and EC:AR 4:1 mixed formula showed similar OA refinement effects through potent anti-inflammatory pathways as compared with those of diclofenac treatment, and showed additional chondrocyte proliferating effects on the both femur and tibia AC.

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Culture of canine synoviocytes on porcine intestinal submucosa scaffolds as a strategy for meniscal tissue engineering for treatment of meniscal injury in dogs

Cited by 8 publications

References 51 publications

Adipose-derived mesenchymal stem cells and platelet-rich plasma synergistically ameliorate the surgical-induced osteoarthritis in Beagle dogs

Adipose-derived mesenchymal stem cells and platelet-rich plasma synergistically ameliorate the surgical-induced osteoarthritis in Beagle dogs

Influence of IGF1, TGFβ1, bFGF and G-CSF/M-CSF on the mRNA levels of selected matrix proteins, cytokines, metalloproteinase 3 and TIMP1 in rat synovial membrane cells

Selection of the optimal herbal composition of pomegranate concentrated powder from aqueous extracts of and to treat osteoarthritis in rats

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