Culture of Bacterium Bacillus subtilis as Degradation Agent in Attempt of Sea Water Remediation Contaminated By Petroleum

Abstract

Crude oil spills pose a serious threat to the marine environment. This is due to crude oil, in large part, is composed of aromatic, aliphatic, and alicyclic hydrocarbons which are toxic, carcinogenic, and mutagenic to the marine life. However, the degradation of crude oil spills with bacteria in simulated seawater media is rarely reported. In this study, oil spill in the seawater, especially petroleum, had been successfully degraded by bacterium culture Bacillus subtilis in simulated seawater under 7 and 14 days incubation. Simulated seawater had synthesized based on ASTM D1141-98 for Standard Practice for the Preparation of Substitute Ocean Water. The petroleum recovery was analyzed using Gas chromatography-mass spectrometry. In this research, the optimum recovery value of crude oil degradation by the bacterial culture obtained by octadecadienoic acid compound gave a percentage recovery of 8.20% and 8.87% after 7 and 14 days of incubation, respectively. This result indicated that the B. subtilis culture has the ability to degrade crude oil spill in simulated sea water.

The application of biosurfactant-producing bacteria immobilized in PVA/SA/bentonite bio-composite for hydrocarbon-contaminated soil bioremediation

“…The difference in compounds degraded by bacteria was influenced by the enzymes produced by each bacterium. 15 …”

The Addition of Different Adsorbents In SA-PVA Matrix For Bacillus Subtilis Immobilization on Methylene Blue Decolorization

“…The supernatant was decanted. Then filtrate or bacteria was washed using 5 mL sterile water and mixed by vortex until homogenous [24]. On the other hand, the matrix preparation was handled by dissolved 1 % (w/v) SA into 45 mL water at 100 °C and stirred for 30 mins.…”

Biodecolorization of Methylene Blue by Using <i>Bacillus subtilis</i> Immobilized into SA-PVA-Bentonite Matrix in Mineral Salt Medium and Non-nutritious Medium

“…The biological method uses the role of microbes (bacteria, fungi and microalgae), and it is environmentally friendly [7]. Bacillus subtilis is a species known to degrade synthetic dye wastewater and adsorb synthetic dyes [8][9][10]. B. subtilis can produce lignin peroxidase (LiP), NADH DCIP reductase and laccase enzymes that can degrade complex aromatic compounds into simpler compounds [11][12].…”