The ability of Daedalea dickinsii to decolorize and transform methylene blue (MB) dye was investigated. MB was decolorized in potato dextrose agar medium after adding MB at concentrations of 50, 75, and 100 mg L. D. dickinsii decolorized MB with decolorization index values of 0.92, 0.90, and 0.88 at MB concentrations of 50, 75, and 100 mg L, respectively. The 100 mg L MB concentration was selected for biotransformation in liquid potato dextrose broth medium. D. dickinsii transformed approximately 54% of the MB after a 14-day incubation. 3-(Dimethylamino)-7-(methylamino) phenothiazine (CHNS), 3,7-bis(dimethylamino)-4aH-phenothiazin-5-one (CHNSO), and 4-(dimethylamino)-2-[m(dimethylamino) phenylsulfinyl] benzenamine (CHNSO) were detected as MB metabolic products. This is the first report of MB transformation by the brown-rot fungi D. dickinsii. These results indicate that D. dickinsii can be used to decolorize and biotransform MB dye.

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The current outbreak of Coronavirus disease 2019 (COVID-19) has become a health emergency of international concern since last December 2019. At the same time, the coronavirus pandemic also caused an economic crisis worldwide. All countries worldwide are competing to generate a qualified strategic plan for resolving this problem. Vaccine, treatment, and detection are the keys to control this pandemic. Global attempts to produce a suitable vaccine are ongoing in many laboratories. However, based on the failure of several previous vaccines, such as Ebola and dengue fever, has stimulated researchers to develop an effective and quick treatment. Effective treatment depends on the effectiveness of the detection method. Thus, a real-time, selective, and accurate detection method is urgently needed to detect if a person has been exposed to COVID-19. This paper is designed to discuss the current situation in controlling COVID-19 by several detection methods. The progress of each method is summarized and compared. Other information regarding the virus, such as the origin, transmission, and biomarkers of the virus, is also explained. The case progress of COVID-19 and its future directions also are discussed.

Bacterium Ralstonia pickettii has ability to survive and thrive in low nutrient condition as well as a capability to remediate some pollutants and using them as carbon and energy source. In this study, the ability of R. pickettii on biodegradation of crude oil under high salinity medium was investigated. R. pickettii was pre-incubated in nutrient broth (NB) medium and then, washed and transferred to artificial seawater medium. Crude oil was added to each culture and incubated for 7 and 14 days. The biodegradation of crude oil was analysed using Gas chromatography mass spectrometry (GC-MS). The result showed that R. pickettii had successfully degraded the crude oil in the high salinity artificial seawater. The incubation on 7 and 14 days did not show a significant effect on the number of the degraded compounds. The optimum recovery percent was obtained from the derivation of 2,6,10,14-tetramethyl hexadecane with the recovery percentage of 12.7% and 16.0% for 7 and 14 days respectively. This study indicates that R. picketti can be potentially used for bioremediation of crude oil under high salinity environments.