Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method

Owen, Henry; Wengerd, Donna; Miller, Alexander

doi:10.1007/bf00232516

Cited by 68 publications

(44 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This result shows the medium has relatively strong buffering capacity compared with Khanna and Srivastava (2005) experiment that shows high decrease in pH from 7 to 5.26 after 48 h. This difference may be due to lower level of carbon source that we used (20 g/L) and usage of glucose instead of fructose in initial study. Owen et al (1991) reported that fructose consumption products lower the pH of media more than other source of carbohydrates.…”

Section: Resultsmentioning

confidence: 99%

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Aramvash

Shahabi

Aghjeh

et al. 2015

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Polyhydroxyalkanoates are biodegradable polymer materials that accumulate in numerous bacteria. The polyhydroxybutyrate is the most common type of polyhydroxyalkanoates, which potentially serves as precursor for bioplastic production. The most extensively studied polyhydroxybutyrate producing bacteria is Cupriavidus necator due to its capability to accumulate large amounts of this biopolymer in simple culture medium. Accumulation of polyhydroxyalkanoates granules in the cytoplasm of C. necator significantly depended on pH, aeration, carbon sources, nitrogen sources, and minerals in the culture medium. In the present study, the effect of both nutritional and physical variables on polyhydroxybutyrate production was investigated in order to optimize these conditions. At first, on the basis of one-factor-at-a-time experiments, fructose and ammonium chloride were found to be the most suitable sources of carbon and nitrogen for biopolymer production. Then the most significant factors affecting granules accumulation were recognized as fructose, agitation speed, KH 2 PO 4 , and initial pH using the Plackett-Burman and central composite design. ANOVA analysis showed significant interaction between fructose and agitation speed. After optimization of the medium, compositions for polyhydroxybutyrate production were determined as follows: fructose 35 g/L, KH 2 PO 4 1.75 g/L, MgSO 4 Á7H 2 O 1.2 g/L, citric acid 1.7 g/L, trace element 10 mL/L, initial pH = 7, and agitation speed 175 rpm. Under this optimal culture conditions, the maximum yield of PHB was 7.48 g/L. The present strategies included in this study could be used for PHB production by this bacterium. These results are the highest values of PHB ever obtained from batch culture of C. necator reported so far.

show abstract

Section: Resultsmentioning

confidence: 99%

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Aramvash

Shahabi

Aghjeh

et al. 2015

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Contamination rates were very low (1-2%, data not shown). All media contained MS vitamins (Murashige & Skoog 1962) and were prepared to obtain a post-autoclave pH of5.8 (Owen et aL 1991).…”

Section: Methodsmentioning

confidence: 99%

Haploid plant regeneration from anther cultures of three north american cultivars of strawberry ( Fragaria x ananassa Duch.)

Owen

Miller

1996

Plant Cell Reports

View full text Add to dashboard Cite

plant regeneration from anther cultures of three north american cultivars of strawberry (Fragaria x ananassa Duch.)" (1996) Summary.A study was conducted to maxmuze plant regeneration frequencies from cultured anthers of' Chandler', 'Honeoye', and 'Redchief strawberries (Fragaria x ananassa Duch.). A comparison of auxins (IAA, NAA), cytokinins (BA, BPA, KIN) and carbohydrates (sucrose, glucose, maltose) in MS medium showed that the highest shoot regeneration across cultivars (8%) occurred when using a medium containing 2 mgtl IAA, I mgtl BA, and 0.2 M glucose. A comparison ofMS, NN, and HI inorganic medium (a new formulation based on the anther culture literature) solidified with either agar or gellan gum and containing IAA, BA, and glucose, showed the highest shoot regeneration across cultivars (19%) when using HI and gellan gum. Lastly, media containing FeEDTAyielded more shoots than media containing Fe-Metalosate, and anthers cultured on Fe-EDT A media in darkness for 30d followed by 30d in white light produced more shoots (16% average regeneration) than those cultured on Fe-EDTA media under white or yellow light (16h photoperiod) for the initial 30d (0.3% and 5% respectively). Plants were acclimated ex vitro where they flowered and set :fruit. Chromosome counts of root tip cells confirmed that haploid plants were obtained from all three cultivars.

show abstract

“…Contrary to previously obtained results (Seingre et al, 1991) (Moncousin, 1992), clearly show that the ontogenic, physiological and genotypical states have a considerable importance in the study of these phenomena. The softening effect of fructose on the gelification of culture media has already been evidenced (Owen et al, 1991), but the negative influence has not been evidenced for other plant material. We cannot yet determine whether the cancer symptoms found, which were more prominent as the amount of fructose increased, were provoked by the presence of furfural and hydroxymethyl-furfural, resulting from autoclaving (Uosukainen and Vasara, 1992); we would be more inclined to think that the "cancerisation" of Malus Jork 9 was a consequence of BAP intoxication; this could have been caused by an increased penetration of the plant hormone due to a greater surface in contact with the medium.…”

Section: Introductionmentioning

confidence: 99%

Effects of type of carbohydrate during proliferation and rooting of microcuttings of Malus Jork 9

Moncousin¹,

Ribaux²,

O'Rourke³

et al. 1992

Agronomie

View full text Add to dashboard Cite

show abstract

Culture medium pH is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method

Cited by 68 publications

References 51 publications

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator

Haploid plant regeneration from anther cultures of three north american cultivars of strawberry ( Fragaria x ananassa Duch.)

Effects of type of carbohydrate during proliferation and rooting of microcuttings of Malus Jork 9

Contact Info

Product

Resources

About