Culture-dependent and culture-independent characterization of bacterial community diversity in different types of sandy lands: the case of Minqin County, China

Abstract

Background Minqin is suffering from a serious desertification, whereas the knowledge about its bacterial community is limited. Herein, based on Nitraria tangutorum and Haloxylon ammodendron from Minqin, the bacterial community diversities in fixed sandy land, semi-fixed sandy land and shifting sandy land were investigated by combining with culture-dependent and culture-independent methods. Results Minqin stressed with high salinity and poor nutrition is an oligotrophic environment. Bacterial community in Minqin was shaped primarily by the presence of host plants, whereas the type of plant and sandy land had no marked effect on those, which displayed a better survival in the rhizospheres of N. tangutorum and H. ammodendron. The dominant groups at phyla level were Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, Planctomycetes, Chloroflexi, Acidobacteria and Candidate_division_TM7. The abundance of Firmicutes with ability of desiccation-tolerance was significantly higher in harsh environment, whereas Bacteroidetes were mainly distributed in areas with high nutrient content. The abundances of Proteobacteria and Bacteroidetes were relatively high in the rhizospheres of N. tangutorum and H. ammodendron, which had more plant-growth promoting rhizobacteria. A large number of Actinobacteria were detected, of which the most abundant genus was Streptomyces. The physicochemical factors related to the diversity and distribution of the bacterial community were comprehensively analyzed, such as pH, electrical conductivity, soil organic matter, C/N and sand, and the results indicated that Minqin was more suitable for the growth of N. tangutorum, which should be one of most important sand-fixing plants in Minqin. Conclusions The bacterial community diversities in different types of sandy lands of Minqin were comprehensively and systematically investigated by culture-dependent and culture-independent approaches, which has a great significance in maintaining/restoring biological diversity.

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“…For this culturable micro-organism Foc TR4, cultivation-based method gave a good prediction of its distribution in soil samples. This is observed by Wei et al (2021) in their work on bacterial community of rhizosphere and bulk soils of Minqin Desert (China). Owing to fail of culturing to reproduce the ecological niches and symbiotic relationships many microorganisms encounter in their natural environments which are required for their survival, the large majority of soil microbes are recalcitrant to culturing techniques (Vieira and Nahas, 2005;Nocker et al, 2007).…”

Analysis of the Antioxidant Composition of Low Molecular Weight Metabolites from the Agarolytic Bacterium Alteromonas macleodii QZ9-9: Possibilities for High-Added Value Utilization of Macroalgae

“…Marine bacteria have been regarded as valuable sources for novel bioactivity metabolites with better biocatalytic properties, e.g., high salinity, high pressure, hyperthermostability, barophilicity, alkali resistance, and low temperature, which can serve various biotechnological applications, such as pharmaceutical, food, and cosmetics [ 14 , 18 , 22 , 23 , 24 , 26 , 27 , 28 ]. Agar in the macroalgae accounted for ~60% of dry weight in its cell wall [ 29 ], and the bacteria with the capacity of metabolizing agar as their only carbon and energy source are recognized as agarolytic bacteria.…”

Multi-Costimulatory Pathways Drive the Antagonistic Pseudoalteromonas piscicida against the Dominant Pathogenic Vibrio harveyi in Mariculture: Insights from Proteomics and Metabolomics

“…An inhibitor does not need to be an antibiotic and may instead be involved in bacterium-bacterium communication by regulating metabolism or physiology such as quorum sensing, thereby driving changes in the bacterial community structure ( 10 , 11 ). Additionally, with increased awareness of the quality and safety of aquatic products, the rising concern of antibiotic-resistant pathogens has led to the emergence of alternative disease prevention strategies, such as the application of nonpathogenic bacteria with antagonistic properties as potential alternative biocontrol agents ( 7 , 12 , 13 ). Some antagonistic bacteria against V. harveyi , such as Pseudomonas spp.…”