Culture and regeneration of human neurons after brain surgery

Brewer, Gregory J.; Espinosa, José Antonio Camacho; McIlhaney, Michael P; Pencek, T.; Kesslak, J. Patrick; Cotman, Carl W.; Viel, John J.; McManus, Dennis C

doi:10.1016/s0165-0270(01)00342-9

Cited by 50 publications

(45 citation statements)

References 33 publications

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“…Therefore, the contribution of astrocytes was regarded fundamental in establishing an in vitro cellular model suitable for studying the neurophysiology of adult human nervous system. Human neuronal cells in our primary cultures were supplied with essential metabolites, -in order to minimize cell-mediated toxicity due to high metabolic demands of cultured cells-, and growth support supplements known to consistently support survival without affecting the differentiated neuronal status or stimulating proliferation of astrocytes and other brain cells (Meberg and Miller, 2003;Brewer et al, 2001;Temburni and Jacob, 2001;De Groot et al, 2000;Peng et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Neuronal cultures from embryonic and neonatal neuronal tissue from several animal models have been reported (Corner et al, 2005;Maslov et al, 2004;Caldwell et al, 2001). Human neuronal cell culture research has focused on the usage of either multipotent embryonic and neonatal stem cells that can be induced to differentiate in vitro (Hammond et al, 2002;Song et al, 2002) or post-mortem brain tissue (Verwer et al, 2002;Brewer et al, 2001;De Groot et al, 2000). Viability and dedifferentiation of adult neurons in vitro have so far been the main disadvantages deterring application of the accomplishments from various animal species to humans.…”

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confidence: 99%

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An in vitro model for investigating human autologous neuronal–astrocyte and immune cell interactions underlying neurodegenerative and immunosuppressive processes in neuropathy

Derventzi¹,

Nikolopoulou²,

Apostolou³

et al. 2014

Brain Research

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

An in vitro model for investigating human autologous neuronal–astrocyte and immune cell interactions underlying neurodegenerative and immunosuppressive processes in neuropathy

Derventzi¹,

Nikolopoulou²,

Apostolou³

et al. 2014

Brain Research

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“…These include sharp electrode and patch clamp neurophysiology, immunocytology, neuron cell signaling, development, excitotoxicity, synaptogenesis, neurotoxicology, neuronal networks, adult rat and mouse neuron culture (Brewer & Torricelli, 2007) and adult human neuron culture (Brewer et al, 2001). Possible limitations might arise for explicit studies of the effects of estrogen, cholesterol or creatine, but these can be controlled by comparisons to formulations of NbActiv4 with these compounds omitted.…”

Section: Discussionmentioning

confidence: 99%

NbActiv4 medium improvement to Neurobasal/B27 increases neuron synapse densities and network spike rates on multielectrode arrays

Brewer

Boehler

Jones

et al. 2008

Journal of Neuroscience Methods

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The most interesting property of neurons is their long-distance propagation of signals as spiking action potentials. Since 1993, Neurobasal/B27 ™ has been used as a serum-free medium optimized for hippocampal neuron survival. Neurons on microelectrode arrays (MEA) were used as an assay system to increase spontaneous spike rates in media of different compositions. We find spike rates of 0.5/second (Hz) for rat embryonic hippocampal neurons cultured in Neurobasal/B27, lower than cultures in serum-based media and offering an opportunity for improvement. NbActiv4™ was formulated by addition of creatine, cholesterol and estrogen to Neurobasal/B27 that synergistically produced an 8-fold increase in spontaneous spike activity. The increased activity with NbActiv4 correlated with a 2-fold increase in immunoreactive synaptophysin bright puncta and GluR1 total puncta. Characteristic of synaptic scaling, immunoreactive GABA Aβ puncta also increased 1.5-fold and NMDA-R1 puncta increased 1.8-fold. Neuron survival in NbActiv4 equaled that in Neurobasal/ B27, but with slightly higher astroglia. Resting respiratory demand was decreased and demand capacity was increased in NbActiv4, indicating less stress and higher efficiency. These results show that NbActiv4 is an improvement to Neurobasal/B27 for cultured networks with an increased density of synapses and transmitter receptors which produces higher spontaneous spike rates in neuron networks.

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“…Survival and regeneration of axons and dendrites were inhibited by large amounts of cellular debris produced during the dissociation procedure (Brewer et al 2001). Debris could further interfere with cell attachment.…”

Section: Discussionmentioning

confidence: 99%

Culture of major pelvic ganglion neurons from adult rat

et al. 2013

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Successful culturing of neurons from adult animals has been historically difficult for a relatively long time. In this study, we reported the development of a novel method for the isolation and the culture of major pelvic ganglion (MPG) neurons from adult rat. The cultured cells were identified by neuron morphology and staining with neuronal marker ). The results demonstrate that the new protocol we used was reliable in obtaining a relatively high yield of MPG neurons. Furthermore, it improves the speed and simplicity in neuronal isolation. The viability of neurons can be maintained for about 2 weeks, which should be sufficient for investigating physiological and pathological processes occurring in mature major pelvic ganglia. And this may provide a useful assessment to currently available techniques for the culture of adult neurons.

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Culture and regeneration of human neurons after brain surgery

Cited by 50 publications

References 33 publications

An in vitro model for investigating human autologous neuronal–astrocyte and immune cell interactions underlying neurodegenerative and immunosuppressive processes in neuropathy

An in vitro model for investigating human autologous neuronal–astrocyte and immune cell interactions underlying neurodegenerative and immunosuppressive processes in neuropathy

NbActiv4 medium improvement to Neurobasal/B27 increases neuron synapse densities and network spike rates on multielectrode arrays

Culture of major pelvic ganglion neurons from adult rat

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