An in vitro model for investigating human autologous neuronal–astrocyte and immune cell interactions underlying neurodegenerative and immunosuppressive processes in neuropathy

Derventzi, Anastasia; Nikolopoulou, Μarilena; Apostolou, Anastasia; Kataki, Agapi; Bakopoulos, Konstantinos; Androulis, Antonios; Kilidireas, Constantinos; Zografos, George; Konstadoulakis, Manousos M.

doi:10.1016/j.brainres.2014.08.058

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…Our finding that the conditioned medium of irradiated neural cells promotes the proliferation of immune cells suggests that soluble factors (e.g., cytokines and chemokines) released by neural cells mediate the communication between the central nervous and immune systems. This premise agrees with the results of others [ 39 , 40 , 41 ]. Notably, the effectiveness of the conditioned medium depended on a combination of neural cells and the types of immune cells targeted.…”

Section: Discussionsupporting

confidence: 94%

Carbon Ion Irradiated Neural Injury Induced the Peripheral Immune Effects in Vitro or in Vivo

Lei

Zhao

et al. 2015

IJMS

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Carbon ion radiation is a promising treatment for brain cancer; however, the immune system involved long-term systemic effects evoke a concern of complementary and alternative therapies in clinical treatment. To clarify radiotherapy caused fundamental changes in peripheral immune system, examinations were performed based on established models in vitro and in vivo. We found that brain-localized carbon ion radiation of neural cells induced complex changes in the peripheral blood, thymus, and spleen at one, two, and three months after its application. Atrophy, apoptosis, and abnormal T-cell distributions were observed in rats receiving a single high dose of radiation. Radiation downregulated the expression of proteins involved in T-cell development at the transcriptional level and increased the proportion of CD3+CD4−CD8+ T-cells in the thymus and the proportion of CD3+CD4+CD8− T-cells in the spleen. These data show that brain irradiation severely affects the peripheral immune system, even at relatively long times after irradiation. In addition, they provide valuable information that will implement the design of biological-based strategies that will aid brain cancer patients suffering from the long-term side effects of radiation.

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Section: Discussionsupporting

confidence: 94%

Carbon Ion Irradiated Neural Injury Induced the Peripheral Immune Effects in Vitro or in Vivo

Lei

Zhao

et al. 2015

IJMS

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Glia–neuron interactions in neurological diseases: Testing non-cell autonomy in a dish

Meyer

Kaspar

2017

Brain Research

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For the past century, research on neurological disorders has largely focused on the most prominently affected cell types – the neurons. However, with increasing knowledge of the diverse physiological functions of glial cells, their impact on these diseases has become more evident. Thus, many conditions appear to have more complex origins than initially thought. Since neurological pathologies are often sporadic with unknown etiology, animal models are difficult to create and might only reflect a small portion of patients in which a mutation in a gene has been identified. Therefore, reliable in vitro systems to studying these disorders are urgently needed. They might be a pre-requisite for improving our understanding of the disease mechanisms as well as for the development of potential new therapies. In this review, we will briefly summarize the function of different glial cell types in the healthy central nervous system (CNS) and outline their implication in the development or progression of neurological conditions. We will then describe different types of culture systems to model non-cell autonomous interactions in vitro and evaluate advantages and disadvantages.

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Early Immune Response Regulated by a Bone Marrow Mesenchymal Stem Cell Model of Multiple Trauma in Rats

et al. 2018

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An in vitro model for investigating human autologous neuronal–astrocyte and immune cell interactions underlying neurodegenerative and immunosuppressive processes in neuropathy

Cited by 3 publications

References 41 publications

Carbon Ion Irradiated Neural Injury Induced the Peripheral Immune Effects in Vitro or in Vivo

Carbon Ion Irradiated Neural Injury Induced the Peripheral Immune Effects in Vitro or in Vivo

Glia–neuron interactions in neurological diseases: Testing non-cell autonomy in a dish

Early Immune Response Regulated by a Bone Marrow Mesenchymal Stem Cell Model of Multiple Trauma in Rats

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