Cultural and Biochemical Characteristics of the Genus Chromobacterium

Sneath, P. H. A.

doi:10.1099/00221287-15-1-70

Cited by 161 publications

(76 citation statements)

References 26 publications

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“…110 and aesculin-azide agar which was prepared according to Sneath (1956). Faeces of animals contaminated with streptomycin-and neomycin-resistant strains of E. coli, Ps.…”

Section: Faeces Culturesmentioning

confidence: 99%

Colonization resistance of the digestive tract in conventional and antibiotic-treated mice

Waaij

Vries

Wees

1971

J. Hyg.

777

378

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SUMMARYThe effect of oral administration of antibiotics on the intestinal flora of conventional mice and their resistance to colonization by orally introduced Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa was studied. Colonization resistance (CR) was expressed as the log of the oral bacterial dose followed by a persistent take in 50 % of the contaminated animals. The intestinal flora was virtually eliminated by the antibiotics and this elimination was accompanied by a precipitous fall of CR. CR gradually returned to normal values during the period of repopulation of the intestinal tract by the organisms surviving the treatment. Antibiotic treatment resulted in the disappearance of Enterobacteriaceae, enterococci, staphylococci and yeasts and, under appropriate housing conditions, the animals remained free of these organisms indefinitely. Germ-free mice contaminated with the intestinal flora of an antibiotic-treated animal and their offspring housed in a germ-free isolator showed high values of CR. Their intestinal flora consisted of anaerobic bacteria only. Apparently, these anaerobes are responsible for CR in these and in conventional mice.

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“…110 and aesculin-azide agar which was prepared according to Sneath (1956). Faeces of animals contaminated with streptomycin-and neomycin-resistant strains of E. coli, Ps.…”

Section: Faeces Culturesmentioning

confidence: 99%

Colonization resistance of the digestive tract in conventional and antibiotic-treated mice

Waaij

Vries

Wees

1971

J. Hyg.

777

378

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“…Besiyeri renginin siyah-kahverengi bir renk alması pozitif olarak değerlendirilmiştir (Sneath, 1956).…”

Section: E) Eskulin (Aesculin)'in Hidroliziunclassified

Batı Akdeniz Bölgesi örtüaltı yetiştiriciliğinde sorun olan domateste Bakteriyel Benek (Pseudomonas syringae pv. tomato “Okabe” Y.D.&W) hastalık etmeni izolatlarının genetik farklılıklarının moleküler yöntemlerle tespiti

Ünlü¹,

Baysal²,

Polat³

et al. 2017

Derim

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The aim of this study was to diagnos bacterial speck of tomato (Pseudomonas syringae pv. tomato) pathogen using biochemical and molecular methods and to determine genetical diversity of Pst isolates by using ISSR and SRAP molecular markers. In the study, survey studies were carried out in West Mediterranean Region to collect bacterial pathogen of tomato (Pst). 10 Pst isolates were collected during survey studies. After isolation, the pathogen was diagnosed with biochemical and molecular diagnostic methods. Besides, genetic diversities of Pst isolates were determined using ISSR and SRAP molecular markers. As a result, bacterial speck of tomato was seen depending on the climatic conditions at the region and Pst was successfully detected by using classical and molecular methods. ISSR and SRAP markers were successively used for analyzing genetic diversity of Pst isolates and these primers could be efficiently used to separate isolates of disease agent.

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“…The mouse was selected as experimental animal because the mouse intestinal flora has been found to be interchangeable with the human intestinal flora as far as its contribution to the CR is concerned (Van der Waaij et al 1977b). (Sneath, 1956) provided information concerning the concentration of Str. faecalis.…”

Section: Introductionmentioning

confidence: 99%

The dose at which neomycin and polymyxin B can be applied for selective decontamination of the digestive tract in mice

Emmelot

Waaij

1980

J. Hyg.

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SUMMARYOral treatment of mice with various doses of neomycin or polymyxin B was performed in order to determine which dose caused substantial suppression of aerobic gram-negative rods. In addition the effect of the various doses on Streptococcus faecalis and on other factors of the colonization resistance (CR) of the digestive tract were studied. It was found that polymyxin B was effective in suppressing sensitive gram-negative bacteria following daily doses of 3-2 mg/ mouse, and that even extremely high daily doses of 9 7 mg/mouse did not affect the CR. Neomycin was effective in suppressing Enterobacteriaceae species following oral daily doses of 5-4 mg/mouse. With this dose, however, the CR was somewhat decreased which was also evidenced by the increased concentration of beta-aspartylglycine in the faeces and the increased size (weight) of the caecum in these animals. Suppression of Str. faecalis was seen from doses of 24 mg/mouse on.

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Cultural and Biochemical Characteristics of the Genus Chromobacterium

Cited by 161 publications

References 26 publications

Colonization resistance of the digestive tract in conventional and antibiotic-treated mice

Colonization resistance of the digestive tract in conventional and antibiotic-treated mice

Batı Akdeniz Bölgesi örtüaltı yetiştiriciliğinde sorun olan domateste Bakteriyel Benek (Pseudomonas syringae pv. tomato “Okabe” Y.D.&W) hastalık etmeni izolatlarının genetik farklılıklarının moleküler yöntemlerle tespiti

The dose at which neomycin and polymyxin B can be applied for selective decontamination of the digestive tract in mice

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