Batı Akdeniz Bölgesi örtüaltı yetiştiriciliğinde sorun olan domateste Bakteriyel Benek (Pseudomonas syringae pv. tomato “Okabe” Y.D.&W) hastalık etmeni izolatlarının genetik farklılıklarının moleküler yöntemlerle tespiti

Abstract

The aim of this study was to diagnos bacterial speck of tomato (Pseudomonas syringae pv. tomato) pathogen using biochemical and molecular methods and to determine genetical diversity of Pst isolates by using ISSR and SRAP molecular markers. In the study, survey studies were carried out in West Mediterranean Region to collect bacterial pathogen of tomato (Pst). 10 Pst isolates were collected during survey studies. After isolation, the pathogen was diagnosed with biochemical and molecular diagnostic methods. Besides, genetic diversities of Pst isolates were determined using ISSR and SRAP molecular markers. As a result, bacterial speck of tomato was seen depending on the climatic conditions at the region and Pst was successfully detected by using classical and molecular methods. ISSR and SRAP markers were successively used for analyzing genetic diversity of Pst isolates and these primers could be efficiently used to separate isolates of disease agent.

Farklı konukçulardan elde edilen Botrytis cinerea populasyon yapısının moleküler tanılanması

“…2.2.1. ISSR mark ers 885, 890, 887, 886 and 880 (Baysal et al, 2009;Baysal et al, 2011;Baysal et al, 2013), 808, 809, 810, 812, 824, 825, 827, 829, 834, 835, 889, 731 and112 (Polat et al, 2014;Ünlü et al, 2017) primers were used to analysis. Amplifications were carried out in reaction volumes of 15 μl containing 1μl DNA (50 nm) template DNA, 1μl dNTP (0.1 mM dNTPs), 1.5 μl MgCl 2 (2.5 mM MgCl 2 ), 0.2 μl Taq (0.6 U Taq DNA polymerase), 2 μl primer (0.3 μM primer), and 72°C for 90 s (extension), followed by a final extension step at 72°C for 10 min.…”