Cultivation Studies of Stevia rebaudiana Bertoni in Turkey

Turgut, Kenan; Özyiğit, Yaşar; Uçar, Esra; Tütüncü, Begüm

doi:10.1055/s-0033-1352454

Cited by 3 publications

(1 citation statement)

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“…China is the largest stevia producer in the world, and the United States and North America are the largest stevia consumers (Al-Taweel et al 2021). This plant, originating from Paraguay and Southwest Brazil, was first introduced in Turkey by being grown in Akdeniz University in Antalya in 2013 (Turgut et al 2013). Stevia is a natural sweetener due to the steviol glycosides it contains.…”

Section: Introductionmentioning

confidence: 99%

Induction of Polyploidy in Stevia Plants (Stevia rebaudiana Bertoni) Using Colchicine

Akbaş,

Turgut

2023

IJIASR

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The primary objective of this study was to establish a protocol for induction of polyploidy in S. rebaudiana var. Levent 93. As a plant material in vitro propagated seedlings of stevia variety Levent 93 were used. For induction of polyploidy, shoot tips with 3-4 leaves of seedlings were immersed in 0.5% concentration of colchicine solution for 1, 2 and 4 hours. Then, shoot tips treated with colchicine solution and distilled water were taken into rooting medium. Plants that developed successfully in the rooting medium were transferred to pots and acclimatized to the external environment. Flow cytometry ploidy analysis was performed on plants treated with colchicine solution after approximately 8 months. According to the ploidy analysis results, it was determined that 17 plants were diploid and 3 plants were tetraploid among 20 plants that survived after treatment with colchicine. All tetraploid plants were observed in 2 hours treatments. As a result of the study, the viability rate, plant height, number of leaves, number of branches, chlorophyll amount, ploidy levels and core DNA contents of stevia plants treated with colchicine were determined and diploid and tetraploid plants were compared. When diploid plants and tetraploid plants are compared, it is concluded that tetraploid plants have smaller averages in terms of plant height average, while they have larger averages in terms of leaf number average and number of branches.

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Section: Introductionmentioning

confidence: 99%