In this study, the possibility of using of chestnut (Castanea sativa) sawdust in Oyster mushroom (Pleurotus ostretatus and Pleurotus citrinopileatus) cultivation was investigated. Additionally; Pleurotus ostreatus cultivation on the substrates which was mixed of chestnut with black poplar (Populus nigra) and oriental spruce (Picea orientalis) was performed. Bioactive properties of these mushroom and their growth mediums were also examined. After a successful harvest, total phenolic, flavonoid, condensed tannin contents and antioxidant properties of mushrooms' methanolic extracts were determined. Same analyses were also performed for mushrooms substrates. The highest yield and biological efficiency was observed in P. ostreatus cultivated on 100% Castanea sativa substrate. The highest total phenolic content (2.529±0.010 mg GAE/g) was found in P. citrinopileatus cultivated on C. sativa sawdust and its substrate medium. Total flavonoid could not determine any mushroom. The highest total condensed tannin (3.691±0.011 CE mg/g) content was observed in P. ostreatus cultivated on C. sativa sawdust and its substrate medium; the highest ferric reducing antioxidant power (11.761±0.020 µmol FeSO 4 .7H 2 O/g) was seen in P. ostreatus mushroom cultivated on 50% P. orientalis + 50% C. sativa and 100% C. sativa substrate medium. The highest free radical scavenging activity of DPPH was in P. citrinopileatus and 100% C. sativa sawdust and 100% C. sativa substrate medium. Keywords: Antioxidant, chestnut, Pleurotus, tannin content, total phenolic content
ÇEŞİTLİ TALAŞLARDA ÜRETİLEN PLEUROTUS OSTREATUS VE PLEUROTUS CITRINOPILEATUS MANTARLARININ TOPLAM FENOLİK, FLAVONOİD VE TANEN İÇERİKLERİ VE ANTİOKSİDAN ÖZELLİKLERİ ÖzBu çal›flmada Pleurotus ostreatus ve Pleurotus citrinopileatus'un (‹stiridye mantar›/Kay›n mantar›) kestane (Castane asativa) odunu talafl›ndaki üretim olanaklar› üzerinde durulmufltur. Ayr›ca; kestane talafl›n›n; karakavak (Populus nigra) ve do¤u ladini (Picea orientalis) talafllar› ile kar›flt›r›ld›¤› ortamlarda Pleurotus ostreatus üretimi denenmifltir. Üretimlerin ard›ndan elde edilen mantarlar›n ve yetiflme ortamlar›n›n biyoaktif özellikleri araflt›r›lm›flt›r. Baflar›l› bir hasat periyodundan sonra mantarlar›n metanolik eksraktlar› üzerinden toplam fenolik, flavonoid ve kondanse tanen içerikleri ve antioksidan özellikleri belirlenmifltir. Ayn› deneyler mantar subsratlar› için de tekrar edilmifltir. En yüksek verim ve biyolojik etkinlik de¤eri %100 Castanea sativa ortam›nda geliflen P. ostreatus mantar›nda gözlenmifltir. En yüksek fenolik içerik (2.529±0.010 mg GAE/g) C. sativa talafl›nda üretilen P. citrinopileatus mantar›nda ve kendi yetiflme ortam›nda bulunmufltur. Hiç bir mantarda flavonoid içeri¤i tespit edilememifltir. En yüksek kondanse tanen içeri¤i (3.691±0.011 CE mg/g) %100 C. sativa talafl›nda üretilen P. ostreatus'da ve kendi yetiflme substrat›nda; en yüksek demir indirgeyici antioksidan aktivite (11.761±0.020 µmol FeSO 4 .7H 2 O/g) %50 P. orientalis + %50 C. sativa kar›fl›m›nda üretilen P. ostrea...