Cultivation of Papova-Like Virus From Human Brain With Progressive Multifocal Leucoencephalopathy

Padgett, Billie L.; ZuRhein, Gabriele M.; Walker, D. L.; Eckroade, RobertJ.; Dessel, BertH.

doi:10.1016/s0140-6736(71)91777-6

Cited by 1,478 publications

(889 citation statements)

References 9 publications

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“…Until recently, two human polyomaviruses BKV [Gardner et al, 1971] and JCV [Padgett et al, 1971] were known to infect human beings. Both viruses are widespread in the human population, establish a latent infection in the kidney [Chesters et al, 1983], and can be detected in the urine of healthy individuals, where infection is asymptomatic [Brown et al, 1975].…”

Section: Introductionmentioning

confidence: 99%

Identification of the novel KI Polyomavirus in paranasal and lung tissues

Babakir‐Mina¹,

Ciccozzi

Campitelli

et al. 2009

Journal of Medical Virology

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KI is a novel polyomavirus identified in the respiratory secretions of children with acute respiratory symptoms. Whether this reflects a causal role of the virus in the human respiratory disease remains to be established. To investigate the presence of KIV in the respiratory tissue, we examined 20 fresh lung cancer specimens and surrounding normal tissue along with one paranasal and one lung biopsy from two transplanted children. KIV‐VP1 gene was detected in 9/20 lung cancer patients and 2/2 transplanted patients. However, amplification of the sequence coding for the C‐terminal part of the early region of KIV performed on the 11 positive cases was successful only in two malignant lung tissues, one surrounding normal tissue, and 1/2 biopsies tested. Phylogenetic analysis performed on the early region of KIV (including the four Italian isolates), BKV and JCV revealed the presence of three distinct clades. Within the KIV clade two sub‐clades were observed. A sub‐clade A containing the four Italian strains, and a sub‐clade B comprising the Swedish and Australian isolates. Interestingly, the two Italian strains identified in normal tissue clustered together, whereas those detected in malignant tissue fell outside this cluster. In vitro studies are needed to investigate the transforming potential of KIV strains. J. Med. Virol. 81:558–561, 2009. © 2009 Wiley‐Liss, Inc.

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Section: Introductionmentioning

confidence: 99%

Identification of the novel KI Polyomavirus in paranasal and lung tissues

Babakir‐Mina¹,

Ciccozzi

Campitelli

et al. 2009

Journal of Medical Virology

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“…Viruses morphologically indistinguishable from papovavirus have been isolated from man [1,5,8,12]. One of these, BK virus, was isolated from a kidney transplant patient [1,5].…”

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confidence: 99%

Transplacental Transmission of BK Virus in Human

Taguchi

Nagaki

Saito

et al. 1975

Japanese Journal of Microbiology

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“…Polyomavirus seroprevalences appear to persist over time, but decrease slightly after early adulthood 15,16 . Similar seroprevalences could either reflect that antibody levels are generally stable on the individual level or may reflect that antibodies both come and go at similar rates.…”

Section: Antibody Stabilitymentioning

confidence: 99%

Serological Diagnosis of Human Polyomavirus Infection

Lundstig

Dillner

Advances in Experimental Medicine and Biology

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Measurement of antibody titres to the human polyomaviruses BK and JC has for many years had to rely on Haemagglutination inhibition. In recent years, viral serology based on viruslike particles (VLPs) in enzyme immunoassays (EIAs) has become widely used for a variety of viruses. We sought to establish a modern method for serological diagnosis of BK and JC viruses, by using purified yeast-expressed virus like particles (VLP) containing the VP1 major capsid proteins. Antibody titres in assays based on VLPs of BKV (strain SB) showed no correlation to the titres in similar JCV assays. BKV (SB) seropositivity increases rapidly with increasing age of the children and reaches a 98 % seroprevalence at 7-9 years of age, whereas JCV seroprevalences increase more slowly with increasing age reaching 51% positivity among children 9-11 years of age. The antibody levels are almost identical in serial samples taken up to 5 years apart, suggesting that both BKV and JCV VLP seropositivitities are usually stable over time and can be used to measure cumulative exposure to these viruses. Serology using SV40 VLPs showed strong cross-reactivity with human polyomaviruses, in particular with BKV strain AS, and establishing a specific VLP-based serology assay for SV40 required blocking with several hyperimmune sera to the human polyomaviruses. SV40-specific seropositivity also increased with increasing age of children, reaching 14% seroprevalence among children 7-9 years of age, but had limited stability over time in serial samples.

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Cultivation of Papova-Like Virus From Human Brain With Progressive Multifocal Leucoencephalopathy

Cited by 1,478 publications

References 9 publications

Identification of the novel KI Polyomavirus in paranasal and lung tissues

Identification of the novel KI Polyomavirus in paranasal and lung tissues

Transplacental Transmission of BK Virus in Human

Serological Diagnosis of Human Polyomavirus Infection

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