Cultivation of Lobelia Inflata L. Hairy Root Culture in Bioreactor

Bányai, Péter; Bálványos, I.; Kursinszki, L.; Szöke, Éva

doi:10.17660/actahortic.2003.597.36

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…Previously, the biosynthetic activity of cell suspension‐, callus‐, organized‐ and genetically transformed hairy root cultures of L . inflata has been reported . Our more recent collaborative studies were conducted with the goal of introducing this species into cultivation in Hungary.…”

Section: Introductionmentioning

confidence: 99%

“…Previously, the biosynthetic activity of cell suspension-, callus-, organized-and genetically transformed hairy root cultures of L. inflata has been reported. [8][9][10][11][12][13] Our more recent collaborative studies were conducted with the goal of introducing this species into cultivation in Hungary. The field and glass house performance, as well as the secondary metabolite production of L. inflata plants, propagated by in vitro and in vivo methods, were evaluated.…”

Section: Introductionmentioning

confidence: 99%

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HPLC‐ESI‐MS/MS of brain neurotransmitter modulator lobeline and related piperidine alkaloids in Lobelia inflata L

Kursinszki

Szöke

2015

J. Mass Spectrom.

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There is a renewed interest in lobelia alkaloids because of their activity on the central nervous system. Lobeline, the most active of them, a nicotinic receptor ligand and neurotransmitter transporter inhibitor, is a candidate pharmacotherapy for metamphetamine abuse. In the present work, high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry in positive ion mode was used for investigating the alkaloid profile in Lobelia inflata L. Chromatographic separations were achieved on a Gemini C6-phenyl reversed-phase column providing good peak shape and improved selectivity. Being mostly 2,6-disubstituted piperidines, lobelia alkaloids presented abundant [M + H](+) ions with typical fragmentation. Identification was possible from a few specific ions, especially those resulting from excision of one of the substituents. Based on fragmentation pattern of lobeline as reference compound, 52 alkaloids were identified in the aqueous methanolic extract of L. inflata in contrast to the previously known some 20. Structural variability of these alkaloids identified arises basically from their substituents which can be phenyl-2-ketoethyl- or phenyl-2-hydroxyethyl units as well as their methyl-, ethyl- or propyl- homologues attached in different combinations. Several propyl homologue lobelia alkaloids and five hydroxypiperidine derivatives were found in the plant at the first time. In addition to 8-O-esters of 2-monosubstituted piperidine alkaloids previously reported by us in L. inflata, a 3-hydroxy-3-phenylpropanoic acid ester of hydroxyallosedamine ring-substituted was also identified as a new natural product. High-performance liquid chromatography-electrospray ionization tandem mass spectrometry can be successfully applied to Lobeliacae plant samples in the routine screening for new and known bioactive constituents, quality control of the crude drug, lobelia herba, alkaloid production studies, breeding and chemotaxonomy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HPLC‐ESI‐MS/MS of brain neurotransmitter modulator lobeline and related piperidine alkaloids in Lobelia inflata L

Kursinszki

Szöke

2015

J. Mass Spectrom.

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“…Growth regulators and alkaloid precursor amino acids had characteristic effects on biomass formation and secondary metabolite production of hairy root cultures (Bálványos et al 2001(Bálványos et al , 2002. The alkaloid production was increased by cultivation in bioreactor (Bányai et al 2003). Several experiments dealt with the influence of macroelements on the growth and alkaloid production of hairy roots .…”

Section: Introductionmentioning

confidence: 99%

Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L.

Bányai

Vojnich

Máthé

et al. 2014

In Vitro Cell.Dev.Biol.-Plant

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Lobelia inflata L. is a medicinally important species that contains numerous piperidine alkaloids. The main alkaloid lobeline is used as a respiratory stimulant. Recently, there have been studies that report on its effect on the central nervous system, drug abuse, and multidrug resistance. We have studied the piperidine alkaloid formation of in vitro cultivated L. inflata on solid Murashige and Skoog medium. With the aim of increasing the anti-addictive lobeline production, we have investigated the effect of changing MgSO 4 levels of the medium. The compounds (−)-lobeline, norlobelanine, lobelanidine-(−)-8-ethyl-10-phenyl-norlobelionol, (−)-8-ethyl-10-phenyl-lobelionol, norlobeline, lobelidine, and lobelanine were identified by HPLC-tandem mass spectrometry (MS/MS). We studied the effect of changing the MgSO 4 levels on the content of lobeline and its derivatives for in vitro cultivated L. inflata. The highest lobeline content was measured in roots cultured on 185 mg L −1 MgSO 4 -containing medium; this value was 2.5 times higher (232.4 μg g −1 ) than in the control cultures. The greatest (fourfold) increase was observed in lobelidine (71.61 μg g −1 ). Addition of MgSO 4 at 740 mg L −1 resulted in the highest lobeline contents in the herba (aerial tissues; 267.8 μg g −1 ), whereas maximum lobeline production was achieved by a further increase in the MgSO 4 level (1,480 mg L −1 ), due to an intensive increase in biomass production. A slight increase was observed in the amount of lobeline derivatives in the herba grown in 740 mg L −1 MgSO 4 -containing medium. The most significant increase (25%) was recorded in the level of norlobelanine.

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Indian Tobacco (Lobelia inflata L.)

Máthé

2020

Medicinal and Aromatic Plants of the World

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Cultivation of Lobelia Inflata L. Hairy Root Culture in Bioreactor

Cited by 6 publications

References 8 publications

HPLC‐ESI‐MS/MS of brain neurotransmitter modulator lobeline and related piperidine alkaloids in Lobelia inflata L

HPLC‐ESI‐MS/MS of brain neurotransmitter modulator lobeline and related piperidine alkaloids in Lobelia inflata L

Enhancement of the anti-addictive lobeline and related alkaloid production of in vitro micropropagated Lobelia inflata L.

Indian Tobacco (Lobelia inflata L.)

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