Cultivation and Transplantation of Epidermal Keratinocytes

Terskikh, V. V.; Vasiliev, A. V.

doi:10.1016/s0074-7696(08)61565-x

Cited by 29 publications

(16 citation statements)

References 136 publications

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“…In relatively limited instances, under the influence of appropriate microenvironmental signals, cultured cells can re-create a tissue architecture resembling that of the original tissue, as occurs for autologous epidermal layers used for grafting purposes in burned patients [1]. The mechanisms controlling the three-dimensional assembly of cells to give rise to differentiated tissues and organs are a crucial issue in stem cell biology as well as regenerative medicine.…”

Section: Importance Of Correct Three-dimensional Organization Of Multmentioning

confidence: 99%

New insights into the morphogenic role of stromal cells and their relevance for regenerative medicine. lessons from the heart

Bani

Nistri

2014

J Cellular Molecular Medi

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The term stromal cells is referred to cells of direct or indirect (hematopoietic) mesenchymal origin, and encompasses different cell populations residing in the connective tissue, which share the ability to produce the macromolecular components of the extracellular matrix and to organize them in the correct spatial assembly. In physiological conditions, stromal cells are provided with the unique ability to shape a proper three-dimensional scaffold and stimulate the growth and differentiation of parenchymal precursors to give rise to tissues and organs. Thus, stromal cells have an essential function in the regulation of organ morphogenesis and regeneration. In pathological conditions, under the influence of local pro-inflammatory mediators, stromal cells can be prompted to differentiate into myofibroblasts, which rather express a fibrogenic phenotype required for prompt deposition of reparatory scar tissue. Indeed, scarring may be interpreted as an emergency healing response to injury typical of evolved animals, like mammals, conceivably directed to preserve survival at the expense of function. However, under appropriate conditions, the original ability of stromal cells to orchestrate organ regeneration, which is typical of some lower vertebrates and mammalian embryos, can be resumed. These concepts underline the importance of expanding the knowledge on the biological properties of stromal cells and their role as key regulators of the three-dimensional architecture of the organs in view of the refinement of the therapeutic protocols of regenerative medicine.

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Section: Importance Of Correct Three-dimensional Organization Of Multmentioning

confidence: 99%

New insights into the morphogenic role of stromal cells and their relevance for regenerative medicine. lessons from the heart

Bani

Nistri

2014

J Cellular Molecular Medi

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“…In tissue repair applications this risk could be reduced by choosing allogeneic cells as target for transgene expression. For example, epithelial sheets of cultured allogeneic cells have been successfully used for the treatment of several skin defects (53). Unlike autografts, grafts of allogeneic cultured cells are not permanently integrated and are thought to persist less than two weeks (54).…”

Section: Recombinant Retrovirusmentioning

confidence: 99%

RETRACTED: Gene therapy and wound healing

Eming

Krieg

Davidson

2007

Clinics in Dermatology

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Wound repair involves the sequential interaction of various cell types, extracellular matrix molecules, and soluble mediators. During the past 10 years, much new information on signals controlling wound cell behavior has emerged. This knowledge has led to a number of novel_therapeutic strategies. In particular, the local delivery of pluripotent growth factor molecules to the injured tissue has been intensively investigated over the past decade. Limited success of clinical trails indicates that a crucial aspect of the growth factor wound-healing strategy is the effective delivery of these polypeptides to the wound site. A molecular approach in which genetically modified cells synthesize and deliver the desired growth factor in regulated fashion has been used to overcome the limitations associated with the (topical) application of recombinant growth factor proteins. We have summarized the molecular and cellular basis of repair mechanisms and their failure, and we give an overview of techniques and studies applied to gene transfer in tissue repair.

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“…As outlined above an alternative approach to the treatment of chronic wounds is the topical application of cytokines and the use of sheets of cultured autologous or allogeneic epidermal cells as a biological dressing [reviewed in Terskikh and Vasiliev, 1999]. One technology that could combine the epidermal cell-based biological dressing with the sustained delivery of high levels of a specific growth factor is retrovirus-mediated gene transfer.…”

Section: In Vitro Gene Transfer Techniquesmentioning

confidence: 99%

Treatment of Chronic Wounds: State of the Art and Future Concepts

Eming

Smola

Krieg

2002

Cells Tissues Organs

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The morbidity and mortality from chronic wounds of varying etiology present a significant health care problem. Multiple local disturbances and systemic disease can impair wound healing. Recently, experiments with tissue cultures and animal models have revolutionized the understanding of wound healing and the pathophysiological processes involved. In cooperation with clinicians and industrial partners novel therapeutic concepts including the topical application of growth factors and cell therapies have been developed. Cytokines that have been tested in clinical studies include epidermal growth factor, platelet-derived growth factor and fibroblast growth factor. These studies showed that an important aspect of the growth factor wound healing paradigm is the effective delivery of these polypeptides to the wound site. Current drug delivery strategies suffer from the inherent loss of drug activity due to the combined effects of physical inhibition and biological degradation. A molecular genetic approach in which genetically modified cells synthesize and deliver the desired growth factor in a time-regulated manner is a powerful means to overcome the limitations associated with the topical application of recombinant growth factor proteins.

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Cultivation and Transplantation of Epidermal Keratinocytes

Cited by 29 publications

References 136 publications

New insights into the morphogenic role of stromal cells and their relevance for regenerative medicine. lessons from the heart

New insights into the morphogenic role of stromal cells and their relevance for regenerative medicine. lessons from the heart

RETRACTED: Gene therapy and wound healing

Treatment of Chronic Wounds: State of the Art and Future Concepts

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