RETRACTED: Gene therapy and wound healing

Abstract

Wound repair involves the sequential interaction of various cell types, extracellular matrix molecules, and soluble mediators. During the past 10 years, much new information on signals controlling wound cell behavior has emerged. This knowledge has led to a number of novel_therapeutic strategies. In particular, the local delivery of pluripotent growth factor molecules to the injured tissue has been intensively investigated over the past decade. Limited success of clinical trails indicates that a crucial aspect of the growth factor wound-healing strategy is the effective delivery of these polypeptides to the wound site. A molecular approach in which genetically modified cells synthesize and deliver the desired growth factor in regulated fashion has been used to overcome the limitations associated with the (topical) application of recombinant growth factor proteins. We have summarized the molecular and cellular basis of repair mechanisms and their failure, and we give an overview of techniques and studies applied to gene transfer in tissue repair.

Aspectos celulares da cicatrização

“…Essa matriz servirá também, como reservatório de citocinas e fatores de crescimento que serão liberados durante as fases seguintes do processo cicatricial. [7][8] As plaquetas, essenciais à formação desse tampão hemostático, também secretam múltiplos mediadores, incluindo fatores de crescimento, liberados na área lesada. Induzidas pela trombina, ainda sofrem a degranulação plaquetária e liberam vários fatores de crescimento, como o derivado de plaquetas (PDGF), o de crescimento transformante-β (TGF-β), o de crescimento epidérmico (EGF), o de crescimento transformante-a (TGF-α) e o fator de crescimento de células endoteliais (VEGF), além de glicoproteínas adesivas como a fibronectina e trombospondina, que são importantes constituintes da matriz extracelular provisória.…”

Regenerative Skin Wound Healing in Mammals: State-of-the-Art on Growth Factor and Stem Cell Based Treatments

“…In this review we are only discussing the existing cell-based and growth factor-based in vitro and in vivo studies and therapies that are at preclinical and clinical level. For readers interested on the remaining regenerative therapies, which are not included in this review, we would like to refer to previous review papers [40,42,43,44,45]. …”

Heparan Sulfate Proteoglycan Mimetics Promote Tissue Regeneration: An Overview

“…Branski, Pereira et al 2007;Eming, Krieg et al 2007). Skin is easily accessible for genetic manipulations and has cellular constituents with a high turnover that can be an effective target for the transfer of genetic material.…”

“…Excessive inflammation and abnormalities in cell-extracellular matrix interaction are considered important mechanisms responsible for the failure of chronic wounds to heal (Eming, Krieg et al 2007;Menke, Ward et al 2007;Schultz and Wysocki 2009). Fig.…”