Cultivation and modelling of encapsulated Saccharomyces cerevisiae in NaCS-PDMDAAC polyelectrolyte complexes

Mei, Liao; Yao, Shiyin

doi:10.1080/02652040210141101

Cited by 21 publications

(12 citation statements)

References 9 publications

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“…Sodium cellulose sulfate (NaCS, Mw = 633 kDa, 120 kDa, 5 kDa) with different molecular weights were prepared by heterogeneous reaction as described previously in our research [4]. Carbon dioxide (CO 2 , purity 99%) and nitrogen (N 2 , purity 99%) were purchased from Hangzhou Jingong Gas Co. Ltd. (Hangzhou, China).…”

Section: Methodsmentioning

confidence: 99%

“…Also, patients will experience significantly less pain when dosage frequency is reduced. Among these various formulations, hydrophilic matrix microparticles with smooth surface and narrow size distributions are considered to be one of the most significant release systems to be used as carriers for drug delivery and cell attachment [4,5]. One favorable characteristic of hydrophilic polymer is its water-solubility and potential pharmaceutical application since solvent residues in the micronized particles could be * Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

“…Due to its favorable biological properties, including nontoxicity, biocompatibility, biodegradation, hydrophilicity, and good film-forming performance [4,[21][22][23][24][25], NaCS has been received much attention and widely used in many fields, especially in pharmaceutical and biomedical engineering. In particular, NaCS was frequently used as a polyanion encapsulation matrix to immobilize enzymes, microorganisms and animal/plant cells or as a swellable hydrophilic matrix applied in the drug delivery system which forms a gel layer when exposed to aqueous media.…”

Section: Introductionmentioning

confidence: 99%

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Microparticle formation of sodium cellulose sulfate using supercritical fluid assisted atomization introduced by hydrodynamic cavitation mixer

Guan

Yao

et al. 2010

Chemical Engineering Journal

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microparticle formation of sodium cellulose sulfate using supercritical fluid assisted atomization introduced by hydrodynamic cavitation mixer

Guan

Yao

et al. 2010

Chemical Engineering Journal

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“…The long-term operational stability of encapsulated whole-cell enzyme was reported, where the encapsulated whole-cell h-galactosidase using Escherichia coli showed gradually increased activity after 30 batches (Oh and Park, 1998). Mei and Yao (2002) reported that the ethanol productivity of encapsulated S. cerevisiae during the repeated batch reached a maximum rate of 66.4 g/LÁh which was seven times more than that in the free-cell culture.…”

Section: Introductionmentioning

confidence: 96%

Ethanol production from glucose and dilute‐acid hydrolyzates by encapsulated S. cerevisiae

Talebnia

Niklasson

Taherzadeh

2005

Biotech & Bioengineering

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The performance of encapsulated Saccharomyces cerevisiae CBS 8066 in anaerobic cultivation of glucose, in the presence and absence of furfural as well as in dilute-acid hydrolyzates, was investigated. The cultivation of encapsulated cells in 10 sequential batches in synthetic media resulted in linear increase of biomass up to 106 g/L of capsule volume, while the ethanol productivity remained constant at 5.15 (+/-0.17) g/L x h (for batches 6-10). The cells had average ethanol and glycerol yields of 0.464 and 0.056 g/g in these 10 batches. Addition of 5 g/L furfural decreased the ethanol productivity to a value of 1.31 (+/-0.10) g/L x h with the encapsulated cells, but it was stable in this range for five consecutive batches. On the other hand, the furfural decreased the ethanol yield to 0.41-0.42 g/g and increased the yield of acetic acid drastically up to 0.068 g/g. No significant lag phase was observed in any of these experiments. The encapsulated cells were also used to cultivate two different types of dilute-acid hydrolyzates. While the free cells were not able to ferment the hydrolyzates within at least 24 h, the encapsulated yeast successfully converted glucose and mannose in both of the hydrolyzates in less than 10 h with no significant lag phase. However, since the hydrolyzates were too toxic, the encapsulated cells lost their activity gradually in sequential batches.

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“…In order to preserve the 100% viability of the yeast in aqueous suspension, alginate microspheres were prepared by using an extrusion method followed by a drying at mild temperature. Microencapsulation by the extrusion process is a method of choice to protect probiotics from degradation during the gastrointestinal transit [25][26][27][28][29][30][31][32][33][34][35] and sodium alginate at an optimised concentration of 1.8% (w/v), 36) is the most used polymer. It presents the benefits of being non-toxic, biodegradable and biocompatible.…”

Section: Discussionmentioning

confidence: 99%

Formulations for Protecting the Probiotic Saccharomyces boulardii from Degradation in Acidic Condition

Graff

Chaumeil

Boy

et al. 2008

Biological & Pharmaceutical Bulletin

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Saccharomyces boulardii is a nonpathogenic yeast with proven health benefits, some of them depending on its viability. However, the living yeast is sensitive to environmental conditions and its viability is less than 1% in the faeces after oral administration. Therefore, we assessed the survival conditions of S. boulardii in aqueous suspension and in its freeze-dried form and we formulated microspheres with the former and tablets with the latter in order to preserve the viability of the probiotic. While the viability of the yeast in aqueous suspension could be maintained for one year at ؊20°C and ؉5°C, increasing the temperature led to almost total mortality within 14 d at ؉40°C and 4 d at ؉60°C. The viability of the freeze-dried yeast was preserved for one year at ؉25°C without moisture. With 75% relative humidity, the mortality was significant at 28 d at ؉25°C and almost total within 1 d at ؉60°C. In vitro, whereas less than 1% of non-encapsulated or non-tabletted S. boulardii survived after 120 min at pH 1.1, both formulations in microspheres and direct compression enabled to protect the yeast from degradation in HCl and to release it viable at pH 6.8. However, despite a similar release profile from both dosage forms, the compression led to a significant decrease in the viability of the freeze-dried yeast. In conclusion, although both formulations are efficient in protecting S. boulardii in acidic condition, microspheres provide a higher entrapment efficiency and a faster release of the viable probiotic in intestinal condition than matrix tablets.Key words microsphere; tablet; Saccharomyces boulardii; viability conditions; probiotic Biol. Pharm. Bull. 31(2) 266-272 (2008) © 2008 Pharmaceutical Society of Japan * To whom correspondence should be addressed. e-mail: sandrine.graff@univ-paris5.ft Assessment of the Survival of S. boulardii under Different Handling/Storage Conditions Samples of freeze-dried yeast (batch 4) were stored at ϩ25 or ϩ60°C either under aerobic conditions with 75% humidity or with no relative humidity, respectively (using saturated NaCl solution to maintain a constant humidity within the enclosure, or silicate gel to maintain the enclosure without humidity) or anaerobic conditions (nitrogen) for one year. A control sample was stored in ambient conditions for one year (temperature between ϩ15 and ϩ35°C and relative humidity from 35 to 90%). At 0,1,4,7,14,28, 90, 180 d and one year, the freezedried yeast was sampled to evaluate its viability.Samples of the aqueous suspension (batch 1) were stored at Ϫ20, ϩ5, ϩ25, ϩ40 and ϩ60°C under aerobic or anaerobic conditions for one year. At the times cited above, the aqueous suspension was sampled to assess the yeast viability. All the experiments were carried out in triplicate.Determination of Viable Yeasts The viability of the yeast has been defined as the proportion of the cells that are capable of multiplication after incubation in standard microbiological conditions. It was evaluated after serial 10-fold dilutions by a plate culture method u...

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Cultivation and modelling of encapsulated Saccharomyces cerevisiae in NaCS-PDMDAAC polyelectrolyte complexes

Cited by 21 publications

References 9 publications

Microparticle formation of sodium cellulose sulfate using supercritical fluid assisted atomization introduced by hydrodynamic cavitation mixer

Microparticle formation of sodium cellulose sulfate using supercritical fluid assisted atomization introduced by hydrodynamic cavitation mixer

Ethanol production from glucose and dilute‐acid hydrolyzates by encapsulated S. cerevisiae

Formulations for Protecting the Probiotic Saccharomyces boulardii from Degradation in Acidic Condition

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