Cultivating Lentinula edodes on Substrate Containing Composted Sawdust Affects the Expression of Carbohydrate and Aromatic Amino Acid Metabolism-Related Genes

Huang, Xiying; Zhang, Runji; Yang, Qi; Li, Xinzhu; Xiang, Quanju; Zhao, Ke; Ma, Menggen; Yu, Xiumei; Chen, Qiang; Zeng, Xiaofeng; Zhang, Lujun; Penttinen, Petri; Gu, Yunfu

doi:10.1128/msystems.00827-21

Cited by 6 publications

(3 citation statements)

References 58 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Ganoderma lucidum, carbon metabolism pathways, such as glycolysis reactions and the TCA cycle, were also regulated by different nitrogen levels [ 29 ]. In Lentinula edodes, the starch and sucrose metabolism pathways were significantly enriched in a higher nitrogen content substrate [ 30 ]. In this study, most DEGs involved in the starch and sucrose metabolism pathway were significantly upregulated by higher nitrogen levels (A2 or A3).…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Nitrogen Levels on Lignocellulolytic Enzyme Production and Gene Expression under Straw-State Cultivation in Stropharia rugosoannulata

Zhang

Zhuo

Wang

et al. 2023

IJMS

View full text Add to dashboard Cite

Stropharia rugosoannulata has been used in environmental engineering to degrade straw in China. The nitrogen and carbon metabolisms are the most important factors affecting mushroom growth, and the aim of this study was to understand the effects of different nitrogen levels on carbon metabolism in S. rugosoannulata using transcriptome analysis. The mycelia were highly branched and elongated rapidly in A3 (1.37% nitrogen). GO and KEGG enrichment analyses revealed that the differentially expressed genes (DEGs) were mainly involved in starch and sucrose metabolism; nitrogen metabolism; glycine, serine and threonine metabolism; the MAPK signaling pathway; hydrolase activity on glycosyl bonds; and hemicellulose metabolic processes. The activities of nitrogen metabolic enzymes were highest in A1 (0.39% nitrogen) during the three nitrogen levels (A1, A2 and A3). However, the activities of cellulose enzymes were highest in A3, while the hemicellulase xylanase activity was highest in A1. The DEGs associated with CAZymes, starch and sucrose metabolism and the MAPK signaling pathway were also most highly expressed in A3. These results suggested that increased nitrogen levels can upregulate carbon metabolism in S. rugosoannulata. This study could increase knowledge of the lignocellulose bioconversion pathways and improve biodegradation efficiency in Basidiomycetes.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Different Nitrogen Levels on Lignocellulolytic Enzyme Production and Gene Expression under Straw-State Cultivation in Stropharia rugosoannulata

Zhang

Zhuo

Wang

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…However, the cereal ba likely denser in oxidisable organics (14.4 g kg −1 ash), enabling L. edodes to perform sive mineralization and increase the ash content in the residual wheat straw by Although the wheat straw had greater mineralization than sawdust (Figure 2), it w nutritionally sufficient to support competitive production relative to sawdust with tion. However, it included larger amounts of cellulose (Figure 3B) and could provi bohydrates as sources of metabolisable energy [34] to support the appreciable prod of shiitake. Studies on producing shiitake on cereal by-products are preliminary, y provide knowledge to progress the formulation of non-wood substrates.…”

Section: Sensitivity Of the Substrate To Biotransformationmentioning

confidence: 99%

A Comparative Analysis of Biodegradation and Bioconversion of Lentinula edodes and Other Exotic Mushrooms

et al. 2023

View full text Add to dashboard Cite

Mushrooms are capable of bioconverting organic residues into food. Understanding the relationship between high-quality yields and substrate biomass from these residues is critical for mushroom farms when choosing new strains. The objective of this exploratory study was, therefore, to analyze whether exotic mushrooms, namely, Pleurotus eryngii, Flammulina velutipes, and Agrocybe aegerita, could biologically convert the substrate into edible mushrooms as effectively as Lentinula edodes (baseline). Five experiments were carried out. Biological efficiency, biodegradability coefficient, mass balance and chemical characterization of the substrate were evaluated. Strategically hydrating the sawdust enabled L. edodes to achieve the greatest biodegradability and biological efficiency of 0.5 and 94.2 kg dt−1, respectively. The values for L. edodes on wheat straw without hydration were 0.2 and 68.8 kg dt−1, respectively. From 1000 kg of fresh substrate, P. eryngii produced 150.1 kg of edible mushrooms, making it technically competitive with L. edodes on wheat straw (195.9 kg). Hence, P. eryngii was the most reliable option for scaling among the exotic mushrooms. The analytical insights from our study provide further knowledge to advance the field’s prominence in high-throughput mushroom-producing systems, particularly for exotic mushrooms.

show abstract

“…In recent years, transcriptome profiling using RNA-seq has rapidly developed, and there is increased research relevant to fungi [ 13 , 14 ]. Here, a transcriptome analysis of F. fomentarius -degraded angiosperm and gymnosperm deadwood was carried out.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptome Analysis Explores the Mechanism of Angiosperm and Gymnosperm Deadwood Degradation by Fomes fomentarius

Wei,

Xue,

Shi

et al. 2024

JoF

View full text Add to dashboard Cite

In forest ecosystems, most of the soil organic matter is derived from trees, as deadwood lignocellulose and wood-decaying basidiomycetes are the most important decomposers of lignin and cellulose. Fomes fomentarius is one of the most common white-rot fungi colonizing angiosperm trees and is often found in birch deadwood but seldom in pine deadwood. To reveal the mechanism through which F. fomentarius selects angiosperms as its preferred host trees, birch and pinewood sticks were selected for culturing for two months. The weight loss, cellulose and lignin degradation rates, activities of degrading enzymes, and transcriptome analyses of two degradation models were compared and analyzed. The results showed that F. fomentarius-degraded birchwood with higher efficiency than pinewood. A GO enrichment analysis found that more upregulated genes related to the top 30 terms showed a molecular function related to degradation, and most genes belonged to the CAZymes family in F. fomentarius-degraded birchwood. However, pinewood degradation did not show these phenomena. A KEGG pathway analysis also indicated that, for the same pathway, more upregulated genes were involved in birchwood degradation caused by F. fomentarius than in pinewood degradation.

show abstract

Cultivating Lentinula edodes on Substrate Containing Composted Sawdust Affects the Expression of Carbohydrate and Aromatic Amino Acid Metabolism-Related Genes

Cited by 6 publications

References 58 publications

Effects of Different Nitrogen Levels on Lignocellulolytic Enzyme Production and Gene Expression under Straw-State Cultivation in Stropharia rugosoannulata

Effects of Different Nitrogen Levels on Lignocellulolytic Enzyme Production and Gene Expression under Straw-State Cultivation in Stropharia rugosoannulata

A Comparative Analysis of Biodegradation and Bioconversion of Lentinula edodes and Other Exotic Mushrooms

Comparative Transcriptome Analysis Explores the Mechanism of Angiosperm and Gymnosperm Deadwood Degradation by Fomes fomentarius

Contact Info

Product

Resources

About