Cultivating conditions influence lipase production by the edible Basidiomycete Antrodia cinnamomea in submerged culture

Lin, En‐Shyh; Wang, Chee-Chan; Sung, Shu-Chiao

doi:10.1016/j.enzmictec.2005.10.002

Cited by 49 publications

(32 citation statements)

References 21 publications

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“…The reported results indicated that medium IV was the best medium for lipase production ((62.2±0.7) U/g), while medium I ((57.1±0.6) U/g) and medium III ((52.3±0.5) U/g) gave moderate lipase yield (data not shown). The lowest enzyme yield ((44.5±0.3) U/g) was observed with medium II, which is in agreement with the observations of Lin et al (23). Sarkar et al (24) stated that the production of lipase is improved when using complex medium instead of the simple one, while Rodriguez et al (25) reported that lipase formation by Rhizopus hornothallicus was highly affected by medium constituents besides pH, temperature and inoculum size.…”

Section: Effect Of Medium Composition On Lipase Productionsupporting

confidence: 81%

“…Yellow colour can be interpreted as the consequence of pH changes in the media caused by increased level of fatty acids due to their release from triglycerides by the action of lipase. Many investigators reported the validity of the previously mentioned method for the primary screening of lipase from different filamentous fungal strains (23)(24)(25). However, for further confirmation spectrophotometric quantitative method was performed.…”

Section: Qualitative Analysis Of Lipase Production By Isolated Fungalmentioning

confidence: 99%

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Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

El-Ghonemy

El-Gamal

Tantawy

et al. 2017

Food Technol. Biotechnol.

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SummaryThirty isolated fungal strains were screened for lipase production using Phenol Red plates, containing tributyrin as lipidic substrate, and a novel fungus identified genetically as Curvularia sp. DHE 5 was found as the most prominent strain. Various agro-industrial substrates were evaluated as inert supports for lipase production in solid-state fermentation. The highest yield of lipase ((83.4±2.2) U/g on dry mass basis) was reported with wheat bran medium after seven days of fermentation at pH=7.0, temperature of 30 °C, 70 % moisture content, inoculum size of 1.27·10 7 spore/mL and 2 % olive oil as an inducer. Supplementation of the medium with 0.05 % KCl as an ion source further increased lipase production to (88.9±1.2) U/g on dry mass basis. The enzyme was partially purified through ammonium sulphate fractionation (40 %) followed by dialysis, and its optimum pH and temperature were reported at 8.0 and 50 °C, respectively, with remarkable pH and thermal stability.

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Section: Effect Of Medium Composition On Lipase Productionsupporting

confidence: 81%

Section: Qualitative Analysis Of Lipase Production By Isolated Fungalmentioning

confidence: 99%

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

El-Ghonemy

El-Gamal

Tantawy

et al. 2017

Food Technol. Biotechnol.

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“…4 Numerous carbon sources, such as Tween 80, triolein, yeast extract, olive oil, soybean oil, silk oil, sesame oil, coconut oil, and corn oil, have been used as substrates to produce lipases in cultures of bacteria. 5 -11 However, very few investigations have been carried out using glycerol as substrate 12,13 and none using raw glycerol, which is the by-product of the transesterification of vegetable oils with alcohols in the reaction to produce biodiesel. 14 Media optimization for the production of enzymes is an important step for commercial applications and involves the study of a number of physicochemical parameters, such as medium composition, carbon and nitrogen sources selection, pH, temperature, and inoculum size.…”

Section: Introductionmentioning

confidence: 99%

Production of organic solvent tolerant lipase by Staphylococcus caseolyticus EX17 using raw glycerol as substrate

Volpato

Rodrigues

Heck

et al. 2008

J of Chemical Tech & Biotech

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BACKGROUND: In this work we used Plackett-Burman statistical design and central composite design in order to optimize culture conditions for lipase production by Staphylococcus caseolyticus strain EX17 growing on raw glycerol, which was obtained as a by-product of the enzymatic synthesis of biodiesel. The stability of lipase was verified over several organic solvents, such as methanol, ethanol and n-hexane.

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“…Therefore, the presence of these two fatty acids and sodium ions may have contributed to make the sunflower soapstock the best lipase inducer. The increase in enzyme activity is related to the proportion of unsaturated fatty acids greater than that of saturated fatty acids [20], and the ions Ca +2 , Mg +2 , Fe +2 , Na +2 or K +2 contribute to the production of lipase [21].…”

Section: Lipase Production Using Different Inducersmentioning

confidence: 99%

Characterization of Different Oil Soapstocks and Their Application in the Lipase Production by Aspergillus niger under Solid State Fermentation

Santos¹,

Muruci²,

Santos³

et al. 2014

JFNR

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Lipases are enzymes that catalyze the hydrolysis of the ester linkage in a triacylglycerol oil-water interface, while in non-aqueous environments they catalyze reverse reactions (esterification, transesterification and interesterification). They can be produced by a solid state fermentation (SSF) process adding value to agro-industrial residues as alkaline soapstocks that can be used as a lipid substrate for lipase production. The objective of this study was to characterize the soapstocks of canola, sunflower and corn and use these byproducts for fungal lipase production using the mutant strain 11T53A14 of Aspergillus niger. The work was carried out following 2 4-1 and 2 3 experimental designs in the presence and absence of inducers, respectively, aiming to evaluate the influence of some variables in the lipase production. The production by SSF was carried out in aerated columns incubated at 32°C. Among the soapstocks evaluated, the sunflower soapstock was the best inducer for enzyme production, with 201 U/g dm (gram of dry medium) using 10 8 spores/g m (gram of medium) inoculated on wheat bran humidified with 80 mL of liquid (ammonium sulfate solution) that corresponds to a final concentration of 0.5% nitrogen, and 3% inducer. The best lipase activity obtained without soapstock was 182 U/g dm under the same processing conditions as the experiment using soapstock. Although the results with the highest lipase activity with sunflower soapstock and without inducer are close, the Tukey test showed a significant difference of these results, which proves the importance of lipid substrate to improve the lipase activity.

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Cultivating conditions influence lipase production by the edible Basidiomycete Antrodia cinnamomea in submerged culture

Cited by 49 publications

References 21 publications

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

Production of organic solvent tolerant lipase by Staphylococcus caseolyticus EX17 using raw glycerol as substrate

Characterization of Different Oil Soapstocks and Their Application in the Lipase Production by <i>Aspergillus </i><i>n</i><i>iger</i> under Solid State Fermentation

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