Production of organic solvent tolerant lipase by <i>Staphylococcus caseolyticus</i> EX17 using raw glycerol as substrate

Volpato, Giandra; Rodrigues, Rafael C.; Heck, Júlio Xandro; Ayub, Marco Antônio Záchia

doi:10.1002/jctb.1875

Cited by 41 publications

(38 citation statements)

References 29 publications

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“…High levels of lipase production were reported from various microorganisms in the presence of olive oil as carbon source (Ahmed et al 2010;Balan et al 2013;Burkert et al 2004;Gupta et al 2007;Mehta et al 2012;Zaliha et al 2006). Most published experimental data have shown that lipid carbon sources especially natural oils stimulate lipase production, whereas carbon sources that are easily broken down and used by bacteria, have an inhibitory effect and contradict the results obtained by many studies (Burkert et al 2004;Gupta et al 2007;Volpato et al 2008;Zaliha et al 2006). In this study, lipase production enhancement was observed by addition of glucose at low concentration (0.5 %) and 1 % concentration of disaccharides, which could be due to enhanced growth of the bacterium and probably excretion of lipase was directly related to biomass production.…”

Section: Preliminary Optimization Using "One-variable-at-a-time"mentioning

confidence: 85%

Medium-based optimization of an organic solvent-tolerant extracellular lipase from the isolated halophilic Alkalibacillus salilacus

Samaei-Nouroozi

Rezaei²,

Khoshnevis³

et al. 2015

Extremophiles

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A haloalkaliphilic solvent-tolerant lipase was produced from Alkalibacillus salilacus within 48 h of growth in liquid medium. An overall 4.9-fold enhanced production was achieved over unoptimized media after medium optimization by statistical approaches. Plackett-Burman screening suggested lipase production maximally influenced by olive oil, KH2PO4, NaCl, and glucose; and response surface methodology predicted the appropriate levels of each parameter. Produced lipase was highly active and stable over broad ranges of temperature (15-65 °C), pH (4.0-11.0), and NaCl concentration (0-30 %) showing excellent thermostable, pH-stable, and halophilic properties. The enzyme was optimally active at pH 8.0 and 40 °C. Majority of cations, except some like Co(2+) and Al(3+) were positive signals for lipase activity. In addition, the presence of chemical agents and organic solvents with different log P ow was well tolerated by the enzyme. Finally, efficacy of lipase-mediated esterification of various alcohols with oleic acid in organic solvents was studied.

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Section: Preliminary Optimization Using "One-variable-at-a-time"mentioning

confidence: 85%

Medium-based optimization of an organic solvent-tolerant extracellular lipase from the isolated halophilic Alkalibacillus salilacus

Samaei-Nouroozi

Rezaei²,

Khoshnevis³

et al. 2015

Extremophiles

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“…Studies by Rech (2010) using Staphylococcus warneri and glycerol as a carbon source showed excellent results regarding enzymatic activity. Volpato et al (2008) also obtained satisfactory lipase production results with Staphylococcus caseolyticus EX17 growing on crude glycerol obtained from biodiesel synthesis. Colla et al (2010) made a comparative study of lipase production by submerged fermentation and solidstate fermentation using Aspergillus sp.…”

Section: Lipase Productionmentioning

confidence: 85%

LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Costa

Hermann

García-Román

et al. 2017

Braz. J. Chem. Eng.

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-Filamentous fungi can easily degrade agro-industrial wastes in solid-state fermentation processes, synthesizing many important commercial biocompounds, such as lipolytic enzymes. The aim of this study was to evaluate the effect of the composition of the solid culture medium on the production of lipolytic enzymes by the fungus Aspergillus niger. Rice, wheat and soybean bran were mixed to prepare the culture medium, which was supplemented with glucose, glycerol or soybean oil. Four mixture experimental designs were used to find the best medium for enzyme production. According to our results, the highest lipolytic activity values were achieved with a mixture of rice bran and glycerol (19.844 U·g -1 ) or with rice bran only (13.267 U·g -1 ). Thus, the lipolytic enzyme could be produced without any additional carbon source apart from rice bran, although glycerol addition induced a higher production.

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“…NBRC3259 could produce glyceric acid (49.5 g/L) and 28.2 g/L dihydroxyacetone from 174 g/L of CG [125]. Solvent tolerant lipase from CG was found to be produced by Staphylococcus caseolyticus EX17 [126]. Ustilago maydis was able to convert CG to glycolipid-type biosurfactants [127].…”

Section: Other Bioproductsmentioning

confidence: 99%

Biorefinery for Glycerol Rich Biodiesel Industry Waste

2016

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The biodiesel industry has the potential to meet the fuel requirements in the future. A few inherent lacunae of this bioprocess are the effluent, which is 10 % of the actual product, and the fact that it is 85 % glycerol along with a few impurities. Biological treatments of wastes have been known as a dependable and economical direction of overseeing them and bring some value added products as well. A novel eco-biotechnological strategy employs metabolically diverse bacteria, which ensures higher reproducibility and economics. In this article, we have opined, which organisms and what bioproducts should be the focus, while exploiting glycerol as feed.

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Production of organic solvent tolerant lipase by Staphylococcus caseolyticus EX17 using raw glycerol as substrate

Cited by 41 publications

References 29 publications

Medium-based optimization of an organic solvent-tolerant extracellular lipase from the isolated halophilic Alkalibacillus salilacus

Medium-based optimization of an organic solvent-tolerant extracellular lipase from the isolated halophilic Alkalibacillus salilacus

LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Biorefinery for Glycerol Rich Biodiesel Industry Waste

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