Cultivating Chinese Medicinal Plants in Germany: A Pilot Project

Bomme, U.; Bauer, Rudolf; Friedl, Fritz; Heuberger, Heidi; Heubl, Günther; Torres-Londoño, Paula; Hummelsberger, J.

doi:10.1089/acm.2006.6276

Cited by 6 publications

(3 citation statements)

References 3 publications

(3 reference statements)

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“…Accordingly, the increased demand for S. miltiorrhiza and its active compounds exceeds the existing industrial and agricultural supplies. Salvia miltiorrhiza has been successfully introduced and cultivated in non-origin countries, such as Germany [ 3 ], but the effects of these different cultivation conditions on the quality of S. miltiorrhiza have, so far, not been characterised. According to the traditional Chinese medicinal prescription, decoction is the main method for consuming S. miltiorrhiza root compounds.…”

Section: Introductionmentioning

confidence: 99%

The Plant Growth-Promoting Fungus (PGPF) Alternaria sp. A13 Markedly Enhances Salvia miltiorrhiza Root Growth and Active Ingredient Accumulation under Greenhouse and Field Conditions

Zhou

Tang

Guo

2018

IJMS

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Plant growth-promoting fungi (PGPF) have attracted considerable interest as bio-fertilisers due to their multiple beneficial effects on plant quantity and quality and their positive relationship with the ecological environment. Advancements in the development of PGPF for crops and economic plant cultivation applications have been achieved, but such improvements for the use of PGPF with popular medicinal herbs, such as Salvia miltiorrhiza, are rare. In this study, we collected S. miltiorrhiza specimens inhabiting wild, semi-wild, farmland and pot-cultured areas in the Henan province of China and isolated endophytes from the roots, shoots and leaves of these samples. Twenty-eight strains of the dominant genus Alternaria were identified and selected as candidate PGPF. Under greenhouse conditions, Alternaria sp. A13 simultaneously enhanced the dry root biomass and secondary metabolite accumulation of S. miltiorrhiza as the optimal PGPF of the 28 candidate isolates. To further assess the interaction between S. miltiorrhiza and Alternaria sp. A13, the effects on seedlings growth, active ingredient accumulation, and the activity of key enzymes for effective biosynthetic pathways were investigated over a period of six months under field conditions. Compared to uninoculated seedlings, S. miltiorrhiza seedlings colonised by Alternaria sp. A13 showed significant increment of 140% in fresh weight, 138% in dry weight, and enhancement in the contents of total phenolic acid, lithospermic acids A and B (LAA and LAB, respectively) of 210%, 128% and 213%, respectively. Examination of the related enzyme activities showed that the elicitation effect of A13 on LAB accumulation correlated with cinnamic acid 4-hydroxylase (C4H) activity in the phenylpropanoid pathway under field conditions. Our results confirmed that Alternaria sp. A13 not only contributes to the stimulation of S. miltiorrhiza root growth, but also boosts the secondary metabolism, thus demonstrating its application potential as a bio-fertiliser for S. miltiorrhiza cultivation, especially in areas outside of its native growth regions.

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Section: Introductionmentioning

confidence: 99%

The Plant Growth-Promoting Fungus (PGPF) Alternaria sp. A13 Markedly Enhances Salvia miltiorrhiza Root Growth and Active Ingredient Accumulation under Greenhouse and Field Conditions

Zhou

Tang

Guo

2018

IJMS

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“…The fresh leaves of young plants are popular for use in a wild vegetable recipe in southeast China [4], whereas its mature plants are commonly used as a traditional medicinal herb in some Asian and Europe countries, such as China [6], Korea [7], Japan [8], Turkey [9], the Czech Republic [10], Poland [11] and Germany [12]. P. vulgaris is rich in phenolic acids [13].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of Potassium Chloride Nutrition for Proper Growth, Physiological Development and Bioactive Component Production in Prunella vulgaris L

Chen

Zhu

et al. 2013

PLoS ONE

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Prunella vulgaris L. is an important medicinal plant with a variety of pharmacological activities, but limited information is available about its response to potassium chloride (KCl) supplementation. P. vulgaris seedlings were cultured in media with four different KCl levels (0, 1.00, 6.00 and 40.00 mM). Characteristics relating to the growth, foliar potassium, water and chlorophyll content, photosynthesis, transpiration, nitrogen metabolism, bioactive constituent concentrations and yield were determined after three months. The appropriate KCl concentration was 6.00 mM to result in the highest values for dry weight, shoot height, spica and root weight, spica length and number in P. vulgaris. The optimum KCl concentration resulted in a maximum net photosynthetic rate (Pn) that could be associated with the highest chlorophyll content and fully open stomata conductance. A supply of surplus KCl resulted in a higher concentration of foliar potassium and negatively correlated with the biomass. Plants that were treated with the appropriate KCl level showed a greater capacity for nitrate assimilation. The Pn was significantly and positively correlated with nitrate reductase (NR) and glutamine synthetase (GS) activities and was positively correlated with leaf-soluble protein and free amino acid (FAA) contents. Both KCl starvation (0 mM) and high KCl (40.00 mM) led to water loss through a high transpiration rate and low water absorption, respectively, and resulted in increased concentrations of ursolic acid (UA), oleanolic acid (OA) and flavonoids, with the exception of rosmarinic acid (RA). Moreover, the optimum concentration of KCl significantly increased the yields of RA, UA, OA and flavonoids. Our findings suggested that significantly higher plant biomass; chlorophyll content; Pn; stronger nitrogen anabolism; lower RA, UA, OA and flavonoid accumulation; and greater RA, UA, OA and flavonoid yields in P. vulgaris could be expected in the presence of the appropriate KCl concentration (6.00 mM).

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“…At the same time, a number of activities were undertaken to inform users at various levels of the availability of locally produced herbal drugs for CHM and the advantages of the their documented and controlled production. This included publications in scientific and applied journals [12][13][14][15], presentations to farmers, wholesalers, pharmacists, physicians and industrial companies, as well as two workshops in Freising and Graz gathering all users for discussion [16]. Specimens of the field-cultivated herbal drugs were distributed on these occasions and posted to wholesalers and pharmacists by the farmer in charge of sales.…”

Section: Transfer To Farmersmentioning

confidence: 99%