Cultivar identification in upland cotton using RAPD markers

Abstract

Using the random amplified polymorphic DNA (RAPD) method, the identification and the genetic description of 28 upland cotton (Gossypium hirsutum L.) cultivars currently cultivated in Greece was attempted. Based on the results of a preliminary experiment using 50 ten-base arbitrary primers, 24 were selected for the main experiment. DNA bands totaling 181 were observed, 118 (65.2%) of which were polymorphic. On the average, 7.5 DNA bands were amplified per primer, 4.9 of which were polymorphic. The unique identification of all cultivars studied was made possible using 27 specific polymorphic bands (markers) corresponding to 16 primers and a specially constructed key. The genetic similarity of the cultivars was estimated using Jaccard's similarity coefficient, which ranged from 0.614 to 0.922, indicating a relatively narrow genetic base. Cluster analysis by the Unweighted Pair Group Method of Arithmetic means (UPGMA) showed that 21 of the cultivars could be placed into 3 major groups. A similar clustering of the cultivars was obtained using principal coordinate analysis.

Pedigree‐ vs. DNA Marker‐Based Genetic Similarity Estimates in Cotton

“…These COP values from Bowman et al (1997) ranged from 0 to 0.75, their median being 0.051 due to skewness toward low COP values. The RFLP‐GS values had a normal‐shaped distribution, ranging from 0.914 to 1 with a median of 0.975, which reflects the low genetic diversity reported by Brubaker and Wendel (1994) and the narrow ranges of genetic distance and similarity reported by Pillay and Myers (1999), Iqbal et al (2001), Linos et al (2002), and Gutiérrez et al (2002) The distributions of COP and RFLP‐GS values were similar to those observed by Barrett et al (1998) in wheat using AFLP markers.…”

Salt Tolerance and Screening for Genetic Changes in Rice Mutants after Gamma Irradiation Using RAPD and Microsatellite (RAMP) Markers

“…The results in rice from this study suggest that RAPD-PCR was able to identify genetic differences between more distantly related rice cultivars [5], and this has also been reported for other plants [27,28]. These results must be considered and evaluated with some caution since the nature of the RAPD-PCR method means that normally it over estimates genetic differences.…”

Genetic Variations and Cultivar Purity within the G. <i>hirsutum</i> Cultivars via RAPD Marker

“…Nature of genetic material could be responsible for different reports on the degree of perceived variations in cotton [1] [2]. Linos et al [3] reported that variations in the DNA sequences are identified by molecular markers which cannot detect the changes in the products of transcribed genes like biochemical and morphological markers. Moreover, several cultivars in a species showed a range of agroclimatic conditions required for the production and growth of cotton.…”

“…These characteristics should be genetically stable within the cultivars so they will reappear when the cultivars is grown again [3] [4]. Performance differences among cultivars are usually more difficult to identify than morphological features because they are more likely to be affected by the environment or more complex inheritance.…”