Cultivar differentiation of Citri Reticulatae Pericarpium by a combination of hierarchical three-step filtering metabolomics analysis, DNA barcoding and electronic nose

Li, Shang-Zhen; Zeng, Su-Ling; Wu, Yan; Zheng, Guodong; Chu, Chu; Yin, Qiang; Chen, Baizhong; Li, Ping; Lu, Xu; Liu, E‐Hu

doi:10.1016/j.aca.2019.01.004

Cited by 41 publications

(16 citation statements)

References 19 publications

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“…Of these compounds, 19 were identified as potential markers that can be used to differentiate between CRBP and CRCP. Li et al [38] used UPLC-QTOFMS to demonstrate that different citrus cultivars can be distinguished based on 9 species-specific chemical markers including 6 flavanone glycosides and 3 polymethoxyflavones. In another study, they identified 15 chemical markers of citrus varieties using GC-MS-based metabolomics [39].…”

Section: Plos Onementioning

confidence: 99%

Characterization of two closely related citrus cultivars using UPLC-ESI-MS/MS-based widely targeted metabolomics

Wang

Chen

et al. 2021

PLoS ONE

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Citrus cultivars are widely spread worldwide, and some of them only differ by specific mutations along the genome. It is difficult to distinguish them by traditional morphological identification. To accurately identify such similar cultivars, the subtle differences between them must be detected. In this study, UPLC-ESI-MS/MS-based widely targeted metabolomics analysis was conducted to study the chemical differences between two closely related citrus cultivars, Citrus reticulata ‘DHP’ and C. reticulata ‘BZH’. Totally 352 metabolites including 11 terpenoids, 35 alkaloids, 80 phenolic acids, 25 coumarins, 7 lignans, 184 flavonoids and 10 other compounds were detected and identified; Among them, 15 metabolites are unique to DHP and 16 metabolites are unique to BZH. Hierarchical cluster analysis (HCA), principal component analysis (PCA), and orthogonal signal correction and partial least squares-discriminant analysis (OPLS-DA) can be used to clearly discriminate between DHP and BZH. 93 metabolites including 36 down-regulated and 57 up-regulated are significantly different in DHP and BZH. They are mainly involved in the biosynthesis of flavonoids, flavones, flavonols, and isoflavonoids. In addition, the relative content levels of flavonoids, alkaloids, and terpenoids are much higher in the peel of DHP than that of BZH, the presence of which may correlate with the quality difference of the peels. The results reported herein indicate that metabolite analysis based on UPLC-ESI-MS/MS is an effective means of identifying cultivars with different genotypes, especially those that cannot be distinguished based on traditional identification methods.

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Section: Plos Onementioning

confidence: 99%

Characterization of two closely related citrus cultivars using UPLC-ESI-MS/MS-based widely targeted metabolomics

Wang

Chen

et al. 2021

PLoS ONE

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“…Genomic DNA was diluted to 15 ng· μ l −1 and then was amplified by performing polymerase chain reaction (PCR) using 4 pair of universal primers of DNA barcodings listed in Table 2 [ 18 ]. PCR was performed under the following conditions: initial denaturation at 98°C for 2 min, followed by 30 cycles with 98°C denaturation for 10 s, annealing at the melting temperatures (TM) listed in Table 2 for 10 s, and extension at 72°C for 10 s. The final extension step was performed for 5 min at 72°C.…”

Section: Methodsmentioning

confidence: 99%

“…Nevertheless, molecular marker about different cultivars of CRP or different varieties of Citrus reticulata “Chachi” was less studied. Previous studies have reported that the ITS2 region was selected for discrimination of the four CRP cultivars; however, this study did not take the intraspecific variation of Citrus reticulata “Chachi” into consideration [ 18 ]. Between different plants of Citrus reticulata “Chachi,” they showed some difference in the tree age and the variety including big-leaf species small-leaf species.…”

Section: Introductionmentioning

confidence: 99%

Intraspecific DNA Barcoding and Variation Analysis for Citri Reticulatae Pericarpium of Citrus reticulata “Chachi”

Liu

Wang

Chen

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Citri Reticulatae Pericarpium, the desiccative mature peel of Citrus reticulata Blanco or its cultivated varieties, is a national geographical indicated product that has the concomitant function of both medicine and foodstuff. The primary source of Citri Reticulatae Pericarpium is Citrus reticulata “Chachi,” called “Guang chenpi,” while it differs in variety, propagation, grafting rootstock, and tree age, and the hereditary stability of its biological information between intraspecific plants is worthy of our attention. Homologous analysis result of 4 DNA barcodings in the ribosome or the chloroplast showed that the homology of them (ITS2, rbcl, matK, and psbA-trnH) of 22 samples was 100.00%, 99.97%, 99.99%, and 99.81%, respectively, which indicated that 4 DNA barcodes maintained a high degree of genetic stability in Citrus reticulata “Chachi.” Also, ITS2 was considered to identify Citrus reticulata “Chachi” from other varieties because it presented not only low variability within a certain taxon but also a high level of interspecies variability. Simultaneously, variant site detection of Citrus reticulata “Chachi” was analyzed by comparing with the reference Citrus reticulata genome, and 2652697 SNP sites and 533906 InDel sites were detected from whole-genome resequencing data of 22 samples, providing the data resources and theoretical foundation for the future study about the relevant molecular makers of “Guang chenpi.”

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“…Integration of genetics and metabolomics is easily the most common approach to botanical product identification, as outlined in Table 1. There are instances when metabolomics and genetics in combination cannot differentiate between species, so additional analytic approaches are employed, such as electronic noise (141), microscopy (142, 143), high-resolution melting analysis (144), Raman spectroscopy (145), or multiple metabolomic approaches (146). Figure 12 demonstrates that integrating multifaceted -omics approaches can be achieved in a single study to increase the power of distinction and authentication of herbal products (141).…”

Section: Multi-omics Integration For Botanical Controlmentioning

confidence: 99%

Chemometric-Guided Approaches for Profiling and Authenticating Botanical Materials

Abraham

Kellogg

2021

Front. Nutr.

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Botanical supplements with broad traditional and medicinal uses represent an area of growing importance for American health management; 25% of U.S. adults use dietary supplements daily and collectively spent over $9. 5 billion in 2019 in herbal and botanical supplements alone. To understand how natural products benefit human health and determine potential safety concerns, careful in vitro, in vivo, and clinical studies are required. However, botanicals are innately complex systems, with complicated compositions that defy many standard analytical approaches and fluctuate based upon a plethora of factors, including genetics, growth conditions, and harvesting/processing procedures. Robust studies rely upon accurate identification of the plant material, and botanicals' increasing economic and health importance demand reproducible sourcing, as well as assessment of contamination or adulteration. These quality control needs for botanical products remain a significant problem plaguing researchers in academia as well as the supplement industry, thus posing a risk to consumers and possibly rendering clinical data irreproducible and/or irrelevant. Chemometric approaches that analyze the small molecule composition of materials provide a reliable and high-throughput avenue for botanical authentication. This review emphasizes the need for consistent material and provides insight into the roles of various modern chemometric analyses in evaluating and authenticating botanicals, focusing on advanced methodologies, including targeted and untargeted metabolite analysis, as well as the role of multivariate statistical modeling and machine learning in phytochemical characterization. Furthermore, we will discuss how chemometric approaches can be integrated with orthogonal techniques to provide a more robust approach to authentication, and provide directions for future research.

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Cultivar differentiation of Citri Reticulatae Pericarpium by a combination of hierarchical three-step filtering metabolomics analysis, DNA barcoding and electronic nose

Cited by 41 publications

References 19 publications

Characterization of two closely related citrus cultivars using UPLC-ESI-MS/MS-based widely targeted metabolomics

Characterization of two closely related citrus cultivars using UPLC-ESI-MS/MS-based widely targeted metabolomics

Intraspecific DNA Barcoding and Variation Analysis for Citri Reticulatae Pericarpium of Citrus reticulata “Chachi”

Chemometric-Guided Approaches for Profiling and Authenticating Botanical Materials

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