Characterization of two closely related citrus cultivars using UPLC-ESI-MS/MS-based widely targeted metabolomics

Wang, Fu; Chen, Lin; Chen, Shiwei; Chen, Hongping; Li, Youping

doi:10.1371/journal.pone.0254759

Cited by 13 publications

(10 citation statements)

References 35 publications

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“…However, no specific data is reported regarding the content of RGL bioactive compounds. Similar studies have identified bioactive compounds from Mandarin Oranges belonging to the same species as RGL (Wang et al 2021). However, the results of the study showed that the typical compounds in each type of mandarin orange could be different even though they came from the same species (Wang et al 2021).…”

Section: Introductionsupporting

confidence: 53%

“…Similar studies have identified bioactive compounds from Mandarin Oranges belonging to the same species as RGL (Wang et al 2021). However, the results of the study showed that the typical compounds in each type of mandarin orange could be different even though they came from the same species (Wang et al 2021). In addition, most of the reported data regarding the compounds present in the RGL citrus species are the result of HPLC, so it has not been possible to analyze large amounts of bioactive compounds simultaneously (Abeysinghe et al 2007;Mei et al 2021).…”

Section: Introductionsupporting

confidence: 53%

“…Further studies are needed to identify other compounds that may be identified in a wider spectrum of metabolomics studies. The alkaloid group of compounds isolated from Mandarin C. reticulata is also reported to have anti-asthmatic activity (Wang et al 2021). In addition, the alkaloid group of compounds has also been reported to have cytotoxic activity on five human tumor cell lines (Ye et al 2021).…”

Section: Alkaloid Contents In Rgl Orangesmentioning

confidence: 99%

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Short Communication: Analysis of detected metabolites compounds from the crude extract of Rimau Gerga Lebong oranges fruit (Citrus reticulata ‘RGL’) using LC-QTOF-MS/MS

et al. 2022

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Abstract.Yunita E, Kurniati T, Rosa FL, Melati P, LestariN. 2022. Short Communication: Analysis of detected metabolites compounds from the crude extract of Rimau Gerga Lebong orangesfruit (Citrus reticulata ‘RGL’) using LC-QTOF-MS/MS. Biodiversitas 23: 3778-3783. Rimau Gerga Lebong (RGL) oranges are the leading commodity in Bengkulu Province. However, the content of bioactive compounds in RGL oranges has not been widely reported. This study aims to identify the flavonoid, alkaloids, and tannins from the ethanol extract of RGL oranges. The results of further identification will be used as a reference in further research on molecular docking in various biological activities. Chemical compounds were identified using qualitative tandem liquid chromatography quadrupole time of flight mass spectrometry (LC-QTOF-MS/MS). The measurement of vitamin C levels was carried out using High-Performance Liquid Chromatography (HPLC). The results showed 25 identified compounds, consisting of 6 compounds belonging to the alkaloid group, 17 from the flavonoid group, and 2 from the tannin group. The alkaloid group compounds were 2?, 3?, 6exo-trihydroxy nortopane, 2?, 3?-dihydroxy nortopane, isosalsolin,3?-dihydrocadambine, chelidimerine, and oleracein D. The compounds from identified flavonoid group were chebuloside, neokurarinol, rutinoside, flavonone, glucoside, licochalcone, viscumneoside, and pinnatifinoside. The identified tannins are corilagin and moracin. These compounds that have been identified have biological activity potential as antioxidants, anti-asthma, anti-dementia, and anticancer. The vitamin C content of the ethanol extract of RGL oranges was 0.02±0.0001%. This vitamin C supports the antioxidant activity of RGL orange.

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Section: Introductionsupporting

confidence: 53%

Section: Introductionsupporting

confidence: 53%

Section: Alkaloid Contents In Rgl Orangesmentioning

confidence: 99%

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Short Communication: Analysis of detected metabolites compounds from the crude extract of Rimau Gerga Lebong oranges fruit (Citrus reticulata ‘RGL’) using LC-QTOF-MS/MS

et al. 2022

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“…Guo et al detected 134 metabolites grouped into 11 classes in blueberry [ 32 ]. Chen et al identified 407 metabolites in strawberry [ 33 ], while 352 metabolites were detected and identified in citrus [ 34 ]. Ma et al identified 464 metabolites in berry skin [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics Combined with Transcriptomics Analysis Revealed the Amino Acids, Phenolic Acids, and Flavonol Derivatives Biosynthesis Network in Developing Rosa roxburghii Fruit

Jiang

Liu

et al. 2022

Foods

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Rosa roxburghii Tratt. is a specific fruit with high nutritional value and antioxidative activities. However, the key metabolites and their biosynthesis are still unknown. Herein, a main cultivated variety, ‘Guinong 5’ (Rr5), was chosen to analyze the metabolomics of the three developmental stages of R. roxburghii fruit by liquid chromatography–tandem mass spectrometry (LC-MS/MS). A total of 533 metabolites were identified, of which 339 were significantly altered. Total phenols, flavonoids, and amino acids were significantly correlated to at least one in vitro antioxidant activity. The conjoint Kyoto Encyclopedia of Genes and Genomes (KEGG) co-enrichment analysis of metabolome and transcriptome was focused on amino acid, phenylpropanoid, and flavonoid biosynthesis pathways. The amino acid, phenolic acid, and flavonol biosynthesis networks were constructed with 32 structural genes, 48 RrMYBs, and 23 metabolites. Of these, six RrMYBs correlated to 9–15 metabolites in the network were selected to detect the gene expression in six different R. roxburghii genotypes fruits. Subsequently, 21 key metabolites were identified in the in vitro antioxidant activities in the fruits at various developmental stages or in fruits of different R. roxburghii genotypes. We found that four key RrMYBs were related to the significantly varied amino acids, phenolic acids, and flavonol derivatives in the network during fruit development and the key metabolites in the in vitro antioxidative activities in the fruits of six R. roxburghii genotypes. This finding provided novel insights into the flavonoid, polyphenol, and amino acid synthesis in R. roxburghii.

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“…In this study, we adjusted the extraction, characterization, and quantification of metabolites based on Wang et al [35], as briefly stated below.…”

Section: Fresh Amla Fruit Extraction Characterization and Quantificat...mentioning

confidence: 99%

Fresh Phyllanthus emblica (Amla) Fruit Supplementation Enhances Milk Fatty Acid Profiles and the Antioxidant Capacities of Milk and Blood in Dairy Cows

et al. 2022

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The objective of this study was to investigate the effect of a diet supplemented with fresh amla fruit as a natural feed additive on blood metabolic parameters, milk antioxidant capacity, and milk fatty acid (FA) proportions in lactating dairy cows. Eight ruminally cannulated mid-lactation dairy cows were used in a repeated crossover design. The first group of four cows received total mixed ration (TMR) feed without fresh amla fruit (control group). The remaining four cows sequentially supplemented fresh amla fruit (FAF) at three levels (200, 400, then 600 g/d) (treatment group) at 14-day intervals. In second period, control and treatment groups were exchanged. The first ten days were adjusted to diet adaptation for each sub-period, and the last four days for sampling milk and blood. A total of 514 metabolites were detected from FAF using UPLC-ESI-MS/MS. The five main metabolites in FAF were phenolic acids (22%), flavonoids (20%), lipids (20%), amino acids and derivatives (9%), and tannins (7%). Amla fruit supplementation reduced total saturated fatty acid and the omega-6/omega-3 ratio at 200 or 400 g/d FAF dose compared to controls. In addition, amla fruit increased unsaturated FA, such as C20:5 (Eicosapentaenoic acid, EPA) and C22:6 (docosahexaenoic acid, DHA), and branched-chain FA in a dose-dependent manner at 200 or 400 g/d compared to controls. In addition, amla fruit increased the antioxidant capacity biomarkers in the blood, such as superoxide dismutase (SOD) and albumin; this confirms that amla fruit is an excellent antioxidant, inhibiting reactive oxygen species’ (ROS) metabolism, and can thereby protect cells from oxidative stress. Moreover, the most remarkable improvement of ferric reducing-antioxidant power (FRAP) and total antioxidant capacity (TAC) in milk was recorded at 400 g/d FAF doses compared to controls. Therefore, fresh amla fruit doses for lactating cows at 400 g/d on an as-fed basis can be used as an alternative additive feed in dairy cow diets to improve antioxidant capacity, protein efficiency, butter quality, and to produce more desirable milk fatty acid profiles for human consumption.

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Characterization of two closely related citrus cultivars using UPLC-ESI-MS/MS-based widely targeted metabolomics

Cited by 13 publications

References 35 publications

Short Communication: Analysis of detected metabolites compounds from the crude extract of Rimau Gerga Lebong oranges fruit (Citrus reticulata ‘RGL’) using LC-QTOF-MS/MS

Short Communication: Analysis of detected metabolites compounds from the crude extract of Rimau Gerga Lebong oranges fruit (Citrus reticulata ‘RGL’) using LC-QTOF-MS/MS

Metabolomics Combined with Transcriptomics Analysis Revealed the Amino Acids, Phenolic Acids, and Flavonol Derivatives Biosynthesis Network in Developing Rosa roxburghii Fruit

Fresh Phyllanthus emblica (Amla) Fruit Supplementation Enhances Milk Fatty Acid Profiles and the Antioxidant Capacities of Milk and Blood in Dairy Cows

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