Culicidae (Diptera) selection of humans, chickens and rabbits in three different environments in the province of Chaco, Argentina

Stein, Marina; Zalazar, Laura; Willener, Juana A.; Almeida, Francisco Ludueña; Almirón, Walter Ricardo

doi:10.1590/s0074-02762013000500005

Cited by 18 publications

(18 citation statements)

References 43 publications

(67 reference statements)

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“…Similar to our ndings, Mitchell et al (1987) identi ed Mansonia titillans frequently feeding on domestic species including Phasianidae birds and Bovidae [30]. Preference of mosquitoes for vertebrates in nature can be modulated by many variables, including but not limited to relative abundance of vertebrate hosts, reproductive cycles, and host defensive behavior [31]. These ecological factors are di cult to monitor, limiting the understanding of innate behaviors of the vector [32] [33] [34] [35].…”

Section: Discussionsupporting

confidence: 65%

Mosquito-vertebrate Interaction Networks in the Magdalena Medio Region of Colombia

Hoyos¹,

Carrasquilla²,

Leon³

et al. 2021

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Studies on the feeding behavior of hematophagous insects, particularly those of medical importance, are relevant for tracking possible pathogen transmission routes and identifying biases in the choice of vertebrates. We evaluated host selection of blood-feeding mosquitoes in a disturbed forest in the Magdalena Medio valley in Colombia from March 2017 to April 2018, after the introduction of Zika virus to the Americas from the 2015-2016 outbreak. We estimated vertebrate diversity and collected blood-engorged female mosquitoes. Mosquito genomic DNA/RNA was extracted for vertebrate host identification and pathogen detection. We performed conventional PCR and sequencing, using universal primers targeting vertebrate regions of the eukaryotic mitochondrial genome to determine bloodmeal host. Additionally, we tested for the presence of flaviviruses in all mosquito samples with RT-PCR. Based on the identity and quantity of detected bloodmeals, we performed mosquito-vertebrate interaction network analysis and estimated topology metrics. In total, we collected 292 engorged female mosquitoes representing 20 different species. Bloodmeal analyses identified 26 vertebrate species, the majority of which were mammals (N=16; 61.5%). No flavivirus infection was confirmed. Although feeding patterns varied, network analyses showed a high degree of specialization by mosquitoes and revealed ecological and phylogenetic relationships among the host community. We conclude that host selection or preference by mosquitoes is species specific.

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Section: Discussionsupporting

confidence: 65%

Mosquito-vertebrate Interaction Networks in the Magdalena Medio Region of Colombia

Hoyos¹,

Carrasquilla²,

Leon³

et al. 2021

Preprint

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“…interfor could transmit SLEV from birds to mammals and thus fulfill a role of "bridge vector" *14, 34, 37+ as Cx. interfor was recorded in human baited barley traps [37] and Cx. interfor and Cx.…”

Section: Discussionmentioning

confidence: 99%

Culex interfor and Culex saltanensis (Diptera: Culicidae) are susceptible and competent to transmit St. Louis encephalitis virus (Flavivirus: Flaviviridae) in central Argentina

Beranek

Quaglia

Peralta

et al. 2020

Transactions of the Royal Society of Tropical Medicine and Hygiene

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Background St. Louis encephalitis virus (SLEV) is endemic and autochthonous on the American continent. Culex pipiens quinquefasciatus is a vector of SLEV; however, Culex interfor and Culex saltanensis have also been found to be naturally infected with SLEV. The aim of this study was to determine the vector competence of C. interfor and C. saltanensis for SLEV from Argentina compared with C. p. quinquefasciatus. Culex genus is Cx.? Methods Female of the Culex species were orally infected by feeding on viraemic chicks that had been inoculated with SLEV. Abdomens, legs and saliva blood-fed mosquitoes were analysed by viral plaque assay. Results Mosquitoes were susceptible to orally acquired infection, dissemination and transmission of SLEV in the saliva. Conclusions Our results demonstrate that C. saltanensis and C. interfor are susceptible to SLEV and competent for its transmission.

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“…The ornithophilic habit was confirmed, with birds accounting for 77.8% of the identified blood meals, whereas mammals were detected in the remaining cases (only in females collected in urban areas). Given that it is considered as an opportunistic species [61], host selection between these two vertebrate classes has already been observed [60,63], which underscores the influence of the environment on the selection of blood meal sources [59]. Among mammals, the equal proportion of canine and human hosts (Table 5) did not evidence a clear preference in the selection within that class of vertebrate [64].…”

Section: Discussionmentioning

confidence: 99%

“…Species of the subgenus Culex , such as Cx . mollis , are considered as primarily ornithophilic [5], with different species showing variation in their host use [60]. On the other hand, although some species of Melanoconion are primarily specialists [16], others, such as Cx .…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of blood meals in mosquitoes (Diptera, Culicidae) in urban and forested habitats in southern Brazil

et al. 2019

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The study of host associations of mosquitoes (Diptera, Culicidae) provides valuable information to assist in our understanding of a variety of related issues, from their life-history to the entomological surveillance of pathogens. In this study, we identified and characterized mosquito blood meals from both urban and forested areas in the city of Paranaguá, state of Paraná, Brazil, by analyzing the amplification of host DNA ingested by mosquitoes under different storage conditions and digestion levels. Host DNA preservation was evaluated in fresh blood meals according to storage duration (30 to 180 days) and temperature (-20°C / -80°C) and, in digested blood, according the degree of digestion classified on the Sella scale. Molecular analysis of blood meals was based on DNA extraction and amplification of a fragment of the mitochondrial COI gene. We determined that, up to180 days of storage, the evaluated temperatures did not influence the preservation of fresh blood meals DNA, whereas the amplification success was increasingly reduced over the course of the digestion process. The species Anopheles cruzii , Aedes fluviatilis , Aedes scapularis , Psorophora ferox , Culex quinquefasciatus , Culex mollis , and Culex intrincatus , together with specimens representing four subgenera and one genus of Culicidae [ Ae . ( Ochlerotatus ), Cx . ( Culex ), Cx . ( Melanoconion ), Cx . ( Microculex ), and Limatus , respectively] had their blood meals identified. Their diverse host use was evidenced by the identification of 19 species of vertebrate host, namely two amphibians, three mammals and 14 birds. Birds were the most commonly identified host in blood meals. These results not only show the diversity of mosquito hosts, but also underscore the challenges involved in monitoring arboviruses of public health importance, given potential combinations of host use for each mosquito species.

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Culicidae (Diptera) selection of humans, chickens and rabbits in three different environments in the province of Chaco, Argentina

Cited by 18 publications

References 43 publications

Mosquito-vertebrate Interaction Networks in the Magdalena Medio Region of Colombia

Mosquito-vertebrate Interaction Networks in the Magdalena Medio Region of Colombia

Culex interfor and Culex saltanensis (Diptera: Culicidae) are susceptible and competent to transmit St. Louis encephalitis virus (Flavivirus: Flaviviridae) in central Argentina

Molecular identification of blood meals in mosquitoes (Diptera, Culicidae) in urban and forested habitats in southern Brazil

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