Cucurbitacin D isolated from Trichosanthes kirilowii induces apoptosis in human hepatocellular carcinoma cells in vitro

Takahashi, Norito; Yoshida, Yasuhiro; Sugiura, Teiichi; Matsuno, Koji; Fujino, Akihiro; Yamashita, Uki

doi:10.1016/j.intimp.2009.01.006

Cited by 45 publications

(31 citation statements)

References 16 publications

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“…Trichosanthins have proved to be effective in inhibiting cancer growth in vitro. Cucurbitacin D isolated from Trichosanthes kirilowii could be a valuable candidate for anti-hepatocarcinoma drug (Takahashi et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…A previous study demonstrated that the ethyl acetate extract of tian hua fen had a significant protective effect on HeLa cells infected with coxsackie virus B3 (Li et al, 2004). Cucurbitacin D, isolated from Trichosanthes kirilowii, could be a valuable candidate as an antitumor drug (Takahashi et al, 2009). Trichosanthin (TCS) is an active component extracted from the root tubers of traditional Chinese medical herb tian hua fen of the Cucurbitaceae family (Zhang et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells

Lin

Kao

et al. 2010

Pharmaceutical Biology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells

Lin

Kao

et al. 2010

Pharmaceutical Biology

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“…1-50 μg/mL Eichenmüller et al [80] Failed to induce apoptosis against HepG2 cells ↑survivin; ↑Bcl-2 0.1-50 μg/mL Eichenmüller et al [80] Cucurbitacin B Decreased the viability of HepG2 cells ↑apoptosis; ↓Bcl-2; ↓pSTAT3 10 nmol/L-10 μmol/L Zhang et al [83] Exhibited growth inhibitory effects on Bel-7402 cells ↑apoptosis; ⊥S; ↓cyclin D1; ↓cdc2; ↓c-Raf; ↑ERK 1/2 0.01-1000 μmol/L Chan et al [84] Cucurbitacin D Suppressed the growth of Hep3b cells ↑apoptosis; ↑caspase-3; ↑pJNK 0-10 μmol/L Takahashi et al [85] Cucurbitacin D, I Displayed cytotoxicity against Bel-7402 cells…”

Section: +mentioning

confidence: 99%

“…Additional studies indicated cucurbitacin B mediated the inhibition of c-Raf activation without any alteration of the STAT3 phosphorylation. Cucurbitacin D, isolated from Trichosanthes kirilowii, was shown to suppress the growth of Hep3b cells by apoptosis through caspase-3 and phosphorylation of JNK protein [85] . Meng et al [86] isolated cucurbitacins D and I from Elaeocarpus hainanensis and reported strong cytoxic effects of these compounds against Bel-7402 cells.…”

Section: Lupeolmentioning

confidence: 99%

Terpenoids as potential chemopreventive and therapeutic agents in liver cancer

Thoppil

Bishayee²

2011

WJH

278

143

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Despite significant advances in medicine, liver cancer, predominantly hepatocellular carcinoma remains a major cause of death in the United States as well as the rest of the world. As limited treatment options are currently available to patients with liver cancer, novel preventive control and effective therapeutic approaches are considered to be reasonable and decisive measures to combat this disease. Several naturally occurring dietary and non-dietary phytochemicals have shown enormous potential in the prevention and treatment of several cancers, especially those of the gastrointestinal tract. Terpenoids, the largest group of phytochemicals, traditionally used for medicinal purposes in India and China, are currently being explored as anticancer agents in clinical trials. Terpenoids (also called "isoprenoids") are secondary metabolites occurring in most organisms, particularly plants. More than 40 000 individual terpenoids are known to exist in nature with new compounds being discovered every year. A large number of terpenoids exhibit cytotoxicity against a variety of tumor cells and cancer preventive as well as anticancer efficacy in preclinical animal models. This review critically examines the potential role of naturally occurring terpenoids, from diverse origins, in the chemoprevention and treatment of liver tumors. Both in vitro and in vivo effects of these agents and related cellular and molecular mechanisms are highlighted. Potential challenges and future directions involved in the advancement of these promising natural compounds in the chemoprevention and therapy of human liver cancer are also discussed.

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“…30 In addition, cucurbitacin D is known to induce apoptosis and cell cycle arrest in ovarian, cervical, lung, and hepatocellular carcinoma. [38][39][40][41] Therefore, we investigated whether doxorubicin and cucurbitacin D affected the viability of MCF7, MDA-MB-231, and SKBR3 cells. We found that cucurbitacin D and doxorubicin significantly suppressed cell growth in MCF7, MDA-MB-231, and SKBR3 cells.…”

Section: Discussionmentioning

confidence: 99%

Cucurbitacin D exhibits its anti-cancer effect in human breast cancer cells by inhibiting Stat3 and Akt signaling

Hong

Kim

et al. 2018

Eur J Inflamm

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Cucurbitacins are triterpenoids commonly found in Cucurbitaceae and Cruciferae and have long been used in traditional medicine. Cucurbitacins demonstrate anti-inflammatory and anti-cancer activities. We investigated whether cucurbitacin D affects viability in breast cancer cells and its mechanism of action. An MTT assay was used to measure the viability of breast cancer cells. Western blot analysis was used to measure the expression of various modulators, such as p-p53, p-Stat3, p-Akt, and p-NF-κB. Doxorubicin and cucurbitacin D affected the viability of MCF7, MDA-MB-231, and SKBR3 cells. Cucurbitacin D and doxorubicin increased p-p53 expression in MCF7, SKBR3, and MDA-MB-231 cells. Cucurbitacin D suppressed p-Akt, p-NF-κB, and p-Stat3 expression in MCF7, MDA-MB-231, and SKBR3 cells. Doxorubicin alone did not decrease p-Akt and p-Stat3 levels. Cucurbitacin D decreased p-NF-κB and p-Stat3 levels. Doxorubicin in combination with cucurbitacin D increased p-p53 levels and suppressed Akt, NF-κB, Stat3, and Bcl-2 expression more than cucurbitacin D alone. Our results clearly demonstrate that cucurbitacin D could be a useful compound for treating human breast cancer.

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Cucurbitacin D isolated from Trichosanthes kirilowii induces apoptosis in human hepatocellular carcinoma cells in vitro

Cited by 45 publications

References 16 publications

The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells

The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells

Terpenoids as potential chemopreventive and therapeutic agents in liver cancer

Cucurbitacin D exhibits its anti-cancer effect in human breast cancer cells by inhibiting Stat3 and Akt signaling

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Cucurbitacin D isolated from Trichosanthes kirilowii induces apoptosis in human hepatocellular carcinoma cells in vitro

Cited by 45 publications

References 16 publications

The mechanisms of action of Tianhua™on antitumor activity in lung cancer cells

The mechanisms of action of Tianhua™on antitumor activity in lung cancer cells

Terpenoids as potential chemopreventive and therapeutic agents in liver cancer

Cucurbitacin D exhibits its anti-cancer effect in human breast cancer cells by inhibiting Stat3 and Akt signaling

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The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells

The mechanisms of action of Tianhua^™on antitumor activity in lung cancer cells