Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan‐1 Pancreatic Cancer Cell Line

Kim, Myeong-Sun; Lee, Kangwook; Ku, Jin Mo; Choi, Yu‐Jeong; Mok, Kyungyul; Kim, Doo Ri; Cheon, Chunhoo; Ko, Seong‐Gyu

doi:10.1155/2020/6571674

Cited by 13 publications

(13 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quinalizarin, an anthraquinone component isolated from Rubiaceae, has been demonstrated to link ROS generation to MAPK, STAT3, and mitochondrial dynamics and inheritance during cell division, as well as the development and disease NF-κB signaling pathways, leading the MCF7 breast cancer cell line and A549 lung cancer cell line to cell-cycle arrest and caspase-dependent apoptosis [ 102 , 103 ]. Cucurbitacin (CuD), a common phytochemical derived from Trichosanthes kirilowii, was used in Capan-1 pancreatic cancer cell line, demonstrating that the drug-induced ROS production induced G2/M cell-cycle arrest and mediated the p38/MAPK pathway, promoting cell death ( Figure 2 and Figure 3 ) [ 104 ].…”

Section: Hints For Anticancer Therapy: Exploitation Of Mitochondrial Rosmentioning

confidence: 99%

Mitochondrial Dynamics, ROS, and Cell Signaling: A Blended Overview

Brillo

Chieregato

Leanza

et al. 2021

Life

101

View full text Add to dashboard Cite

Mitochondria are key intracellular organelles involved not only in the metabolic state of the cell, but also in several cellular functions, such as proliferation, Calcium signaling, and lipid trafficking. Indeed, these organelles are characterized by continuous events of fission and fusion which contribute to the dynamic plasticity of their network, also strongly influenced by mitochondrial contacts with other subcellular organelles. Nevertheless, mitochondria release a major amount of reactive oxygen species (ROS) inside eukaryotic cells, which are reported to mediate a plethora of both physiological and pathological cellular functions, such as growth and proliferation, regulation of autophagy, apoptosis, and metastasis. Therefore, targeting mitochondrial ROS could be a promising strategy to overcome and hinder the development of diseases such as cancer, where malignant cells, possessing a higher amount of ROS with respect to healthy ones, could be specifically targeted by therapeutic treatments. In this review, we collected the ultimate findings on the blended interplay among mitochondrial shaping, mitochondrial ROS, and several signaling pathways, in order to contribute to the dissection of intracellular molecular mechanisms involved in the pathophysiology of eukaryotic cells, possibly improving future therapeutic approaches.

show abstract

Section: Hints For Anticancer Therapy: Exploitation Of Mitochondrial Rosmentioning

confidence: 99%

Mitochondrial Dynamics, ROS, and Cell Signaling: A Blended Overview

Brillo

Chieregato

Leanza

et al. 2021

Life

101

View full text Add to dashboard Cite

show abstract

“…Among them, cucurbitacin B (approximately 250 µg/g) and CucD (approximately 50 µg/g) were present in the largest quantity [46]. In our study, we focused on CucD because of the cytotoxicity of cucurbitacin B and CucD, and anti-cancer effects in various cancer models have been reported in literature [65][66][67][68]. Despite the observed toxicity of both cucurbitacin B and CucD, the cell viability of endothelial cells of the pulmonary artery (CPAE) was measured after Tk extract inoculation to evaluate the safety of Tk, and Tk did not show any significant toxicity to CPAE (Supplementary Figure S1).…”

Section: Discussionmentioning

confidence: 96%

Analgesic Effect of SH003 and Trichosanthes kirilowii Maximowicz in Paclitaxel-Induced Neuropathic Pain in Mice

Lee

Kim

et al. 2022

CIMB

Self Cite

View full text Add to dashboard Cite

Pacliatxel is a taxol-based chemotherapeutic drug that is widely used to treat cancer. However, it can also induce peripheral neuropathy, which limits its use. Although several drugs are prescribed to attenuate neuropathies, no optimal treatment is available. Thus, in our study, we analyzed whether SH003 and its sub-components could alleviate paclitaxel-induced neuropathic pain. Multiple paclitaxel injections (cumulative dose 8 mg/kg, i.p.) induced cold and mechanical allodynia from day 10 to day 21 after the first injection in mice. Oral administration of SH003, an herbal mixture extract of Astragalus membranaceus, Angelica gigas, and Trichosantheskirilowii Maximowicz (Tk), dose-dependently attenuated both allodynia. However, when administered separately only Tk decreased both allodynia. The effect of Tk was shown to be mediated by the spinal noradrenergic system as intrathecal pretreatment with α1- and α2-adrenergic-receptor antagonists (prazosin and idazoxan), but not 5-HT1/2, and 5-HT3-receptor antagonists (methysergide and MDL-72222) blocked the effect of Tk. The spinal noradrenaline levels were also upregulated. Among the phytochemicals of Tk, cucurbitacin D was shown to play a major role, as 0.025 mg/kg (i.p.) of cucurbitacin D alleviated allodynia similar to 500 mg/kg of SH003. These results suggest that Tk should be considered when treating paclitaxel-induced neuropathic pain.

show abstract

“…It also significantly decreased expression of Cyclin D1, Survivin, XIAP, Bcl2 and Mcl-1 [42]. In the pancreatic cancer cell line Capan-1, CuD induced cell-cycle arrest and death via the ROS/p38 pathway [43]. Cucurbitacin I-induced cell death in ovarian cancer (SKOV3) included apoptosis, as evidenced by upregulated caspase 3 and BAX and a decrease in Bcl2 [21].…”

Section: Apoptotic and Cell-cycle Arrestmentioning

confidence: 98%

Cucurbitacins as Potent Chemo-Preventive Agents: Mechanistic Insight and Recent Trends

et al. 2022

View full text Add to dashboard Cite

Cucurbitacins constitute a group of cucumber-derived dietary lipids, highly oxidized tetracyclic triterpenoids, with potential medical uses. These compounds are known to interact with a variety of recognized cellular targets to impede the growth of cancer cells. Accumulating evidence has suggested that inhibition of tumor cell growth via induction of apoptosis, cell-cycle arrest, anti-metastasis and anti-angiogenesis are major promising chemo-preventive actions of cucurbitacins. Cucurbitacins may be a potential choice for investigations of synergism with other drugs to reverse cancer cells’ treatment resistance. The detailed molecular mechanisms underlying these effects include interactions between cucurbitacins and numerous cellular targets (Bcl-2/Bax, caspases, STAT3, cyclins, NF-κB, COX-2, MMP-9, VEGF/R, etc.) as well as control of a variety of intracellular signal transduction pathways. The current study is focused on the efforts undertaken to find possible molecular targets for cucurbitacins in suppressing diverse malignant processes. The review is distinctive since it presents all potential molecular targets of cucurbitacins in cancer on one common podium.

show abstract

Cucurbitacin D Induces G2/M Phase Arrest and Apoptosis via the ROS/p38 Pathway in Capan‐1 Pancreatic Cancer Cell Line

Cited by 13 publications

References 42 publications

Mitochondrial Dynamics, ROS, and Cell Signaling: A Blended Overview

Mitochondrial Dynamics, ROS, and Cell Signaling: A Blended Overview

Analgesic Effect of SH003 and Trichosanthes kirilowii Maximowicz in Paclitaxel-Induced Neuropathic Pain in Mice

Cucurbitacins as Potent Chemo-Preventive Agents: Mechanistic Insight and Recent Trends

Contact Info

Product

Resources

About