Cucurbit chlorotic yellows virus p22 is a suppressor of local RNA silencing

Orfanidou, C. G.; Mathioudakis, Matthaios M.; Κατσαρού, Κωνσταντίνα; Livieratos, Ioannis C.; Katis, N. I.; Maliogka, Varvara I.

doi:10.1007/s00705-019-04391-x

Cited by 6 publications

(9 citation statements)

References 49 publications

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“…CCYV RNA 1-encoded p22 is a reported weak suppressor of RNA silencing and in coinfiltration experiments in the tissues surrounding the GFP-and p22-infiltrated area, a fine dark zone appeared, indicating there to be no interference with the short-range cell-to-cell movement of RNA silencing signal (Orfanidou et al, 2019). Moreover, CCYV p22 is able to suppress local silencing directly induced by the production of dsRNA, suggesting that it inhibits RNA silencing in parallel with, or downstream of, the production of dsRNA.…”

Section: Discussionmentioning

confidence: 95%

“…In recent years, it has become apparent that a number of proteins encoded by 3'-proximal ORFs of crinivirus RNA 1 are able to suppress RNA silencing (Kreuze et al, 2005;Cañizares et al, 2008;Cuellar et al, 2009;Kataya et al, 2009;Kubota & Ng, 2016;Mashiko et al, 2019;Orfanidou et al, 2019;Chen et al, 2019) or/and to bind RNA (Wang et al, 2010;Weinheimer et al, 2014;Landeo-Ríos et al, 2016). For these proteins, typically expressed from the earliest stages of infection (Klaassen et al, 1996;Salem et al, 2009;Orilio et al, 2014), there is increasing evidence that significant divergence in their manner(s) of action exists.…”

Section: Discussionmentioning

confidence: 99%

“…1) includes 0-3 similarly-sized ORFs encoding proteins that share low levels (25-40%) of amino acid homology. A number of these proteins, including CCYV p22, have been reported to be viral suppressors of RNA silencing (VSRs): Lettuce chlorosis virus p23, Sweet potato chlorotic stunt virus (SPCSV) RNase3 and p22, Tomato chlorosis virus (ToCV) p22, CYSDV p25 and Tomato infectious chlorosis virus p27 (Kreuze et al, 2005;Cañizares et al, 2008;Cuellar et al, 2009;Kataya et al, 2009;Kubota & Ng, 2016;Mashiko et al, 2019;Orfanidou et al, 2019;Chen et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Cucurbit chlorotic yellows virus p22 suppressor of RNA silencing binds single-, double-stranded long and short interfering RNA molecules in vitro

et al. 2020

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cucurbit chlorotic yellows virus p22 suppressor of RNA silencing binds single-, double-stranded long and short interfering RNA molecules in vitro

et al. 2020

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“…In addition to the known RSSs, other viral gene products that might participate in the activity have been selected. For instance, the rather variable P1 of potyviruses (Valli et al, 2007;Shan et al, 2015) that has been considered a modulator of RSS in several viruses (Fernández et al, 2013;Pasin et al, 2014) and the P22 of CYSDV, the gene product downstream of the P25 region in the RNA1, located in a position where other criniviruses encode proteins involved in this function (Kreuze et al, 2005;Cañizares et al, 2008;Weinheimer et al, 2015;Kubota and Ng, 2016;Chen et al, 2019;Orfanidou et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus

et al. 2021

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Mixed viral infections in plants involving a potyvirus and other unrelated virus often result in synergistic effects, with significant increases in accumulation of the non-potyvirus partner, as in the case of melon plants infected by the potyvirus Watermelon mosaic virus (WMV) and the crinivirus Cucurbit yellow stunting disorder virus (CYSDV). To further explore the synergistic interaction between these two viruses, the activity of RNA silencing suppressors (RSSs) was addressed in transiently co-expressed combinations of heterologous viral products in Nicotiana benthamiana leaves. While the strong RSS activity of WMV Helper Component Proteinase (HCPro) was unaltered, including no evident additive effects observed when co-expressed with the weaker CYSDV P25, an unexpected negative effect of WMV P1 was found on the RSS activity of P25. Analysis of protein expression during the assays showed that the amount of P25 was not reduced when co-expressed with P1. The detrimental action of P1 on the activity of P25 was dose-dependent, and the subcellular localization of fluorescently labeled variants of P1 and P25 when transiently co-expressed showed coincidences both in nucleus and cytoplasm. Also, immunoprecipitation experiments showed interaction of tagged versions of the two proteins. This novel interaction, not previously described in other combinations of potyviruses and criniviruses, might play a role in modulating the complexities of the response to multiple viral infections in susceptible plants.

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“…As RNA1 3′ ORFs are quite variable among criniviruses, both P6 and P22 show no significant similarity to corresponding proteins of other criniviruses ( Okuda et al, 2010 ). In addition to CCYV P22, P22 proteins of tomato chlorosis virus (ToCV) and sweet potato chlorotic stunt virus (SPCSV), P23 of lettuce chlorosis virus (LCV), and P25 of cucurbit yellow stunting disorder virus (CYSDV) have also been identified as RNA silencing suppressors ( Kreuze et al, 2005 ; Canizares et al, 2008 ; Kataya et al, 2009 ; Kubota and Ng, 2016 ; Orfanidou et al, 2019 ; Salavert et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Cucumber Ribosomal Protein CsRPS21 Interacts With P22 Protein of Cucurbit Chlorotic Yellows Virus

et al. 2021

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Cucurbit chlorotic yellows virus (CCYV) is a cucurbit-infecting crinivirus. RNA silencing can be initiated as a plant defense against viruses. Viruses encode various RNA silencing suppressors to counteract antiviral silencing. P22 protein encoded by RNA1 of CCYV is a silencing suppressor, but its mechanism of action remains unclear. In this study, the cucumber ribosomal-like protein CsRPS21 was found to interact with P22 protein in vitro and in vivo. A conserved CsRPS21 domain was indispensable for its nuclear localization and interaction with P22. Transient expression of CsRPS21 in Nicotiana benthamiana leaves interfered with P22 accumulation and inhibited P22 silencing suppressor activity. CsRPS21 expression in N. benthamiana protoplasts inhibited CCYV accumulation. Increasing numbers of ribosomal proteins are being found to be involved in viral infections of plants. We identified a P22-interacting ribosomal protein, CsRPS21, and uncovered its role in early viral replication and silencing suppressor activity. Our study increases knowledge of the function of ribosomal proteins during viral infection.

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Cucurbit chlorotic yellows virus p22 is a suppressor of local RNA silencing

Cited by 6 publications

References 49 publications

Cucurbit chlorotic yellows virus p22 suppressor of RNA silencing binds single-, double-stranded long and short interfering RNA molecules in vitro

Cucurbit chlorotic yellows virus p22 suppressor of RNA silencing binds single-, double-stranded long and short interfering RNA molecules in vitro

The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus

Cucumber Ribosomal Protein CsRPS21 Interacts With P22 Protein of Cucurbit Chlorotic Yellows Virus

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