Cucumber Ribosomal Protein CsRPS21 Interacts With P22 Protein of Cucurbit Chlorotic Yellows Virus

Yang, Xue; Ying, Wei; Shi, Yong; Han, Xiaoyu; Chen, Siyu; Yang, Lingling; Li, Honglian; Sun, Bing‐Jian; Shi, Yan

doi:10.3389/fmicb.2021.654697

Cited by 3 publications

(1 citation statement)

References 43 publications

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“…A cDNA library of Eureka lemon was constructed as previously described (Yang et al, 2021 ). Full‐length CYVCV CP (KP313240.1) was amplified with the primers BD‐CP F/R (Table S1 ) and digested with EcoRI and BamHI.…”

Section: Methodsmentioning

confidence: 99%

The interaction between the lemon ribosomal protein ClRPS9‐2 and citrus yellow vein clearing virus coat protein affects viral infection and gene silencing suppressor activity

Zeng

Ping

Zheng

et al. 2023

Molecular Plant Pathology

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Citrus yellow vein clearing virus (CYVCV) is an emerging virus that causes serious economic damage to the lemon industry worldwide. The coat protein (CP) of CYVCV is a strong RNA silencing suppressor and is associated with the severity of symptoms in citrus, yet the interaction between CP and host factors remains unknown. In this study, the 40S ribosomal subunit protein S9‐2 (ClRPS9‐2) was identified as a CP‐binding partner using the yeast two‐hybrid system from a lemon (cv. Eureka) cDNA library, and the interaction between CP and ClRPS9‐2 was demonstrated by in vivo methods. The results suggest that the N‐terminal 8–108 amino acid sequence of ClRPS9‐2 is crucial for its interaction with CP and may be associated with the nuclear localization of ClRPS9‐2. The accumulation and silencing suppressor activity of CP were reduced by transient expression of ClRPS9‐2 in Nicotiana benthamiana. Reverse transcription‐quantitative PCR analysis showed that the content of CYVCV in ClRPS9‐2 transgenic Eureka lemon plants was approximately 50% of that in CYVCV‐infected wild‐type plants 1 month after inoculation, and mild yellowing and vein clearing symptoms were observed in the transgenic plants. These findings demonstrate that ClRPS9‐2 plays a role in host defensive reactions, and the enhanced resistance of transgenic plants to CYVCV may be associated with the up‐regulation of salicylic acid‐related and R genes.

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Section: Methodsmentioning

confidence: 99%

The interaction between the lemon ribosomal protein ClRPS9‐2 and citrus yellow vein clearing virus coat protein affects viral infection and gene silencing suppressor activity

Zeng

Ping

Zheng

et al. 2023

Molecular Plant Pathology

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Synthesis, Anti-TMV Activities, and Action Mechanisms of a Novel Cytidine Peptide Compound

Yu,

Liu,

Wang

et al. 2024

J. Agric. Food Chem.

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Cytidine has a broad range of applications in the pharmaceutical field as an intermediate of antitumor or antiviral agent. Here, a series of new cytidine peptide compounds were synthesized using cytidine and Boc group-protected amino acids and analyzed for their antiviral activities against tobacco mosaic virus (TMV). Among these compounds, the structure of an effective antiviral cytidine peptide SN11 was characterized by 1H NMR, 13C NMR, and high-resolution mass spectrometer. The compound SN11 has a molecular formula of C15H22N6O8 and is named 2-amino-N-(2- ((1- (3,4-dihydroxy-5-(hydroxymethyl) tetrahydrofuran-2-yl) -2-oxo-1,2-dihydropyrimidin-4-yl) amino) -2-oxyethyl) amino). The protection, inactivation, and curation activities of SN11 at a concentration of 500 μg/mL against TMV in Nicotiana glutinosa were 82.6%, 84.2%, and 72.8%, respectively. SN11 also effectively suppressed the systemic transportation of a recombinant TMV carrying GFP reporter gene (p35S-30B:GFP) in Nicotiana benthamiana by reducing viral accumulation to 71.3% in the upper uninoculated leaves and inhibited the systemic infection of TMV in Nicotiana tabacum plants. Furthermore, the results of RNA-seq showed that compound SN11 induced differential expression of genes involved in the biogenesis and function of ribosome, plant hormone signal transduction, plant pathogen interaction, and chromatin. These results validate the antiviral mechanisms of the cytidine peptide compound and provide a theoretical basis for their potential application in the management of plant virus diseases.

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Tropical Fruit Virus Resistance in the Era of Next-Generation Plant Breeding

Vieira,

Cabral,

Favaratto

et al. 2024

SynBio

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Plant viral diseases constitute a major contributor to agricultural production losses, significantly impacting the economies of exporting countries by more than USD 30 billion annually. Understanding and researching the biology and genomics of viruses is crucial for developing virus-resistant genetically edited or genetically modified plants. Genetic modifications can be targeted to specific regions within genes of target plants which are important or essential for the virus to establish a systemic infection, thus fostering resistance or enabling plants to effectively respond to invading agents while preserving their yield. This review provides an overview of viral incidence and diversity in tropical fruit crops and aims to examine the current state of the knowledge on recent research efforts aimed at reducing or eliminating the damage caused by viral diseases, with emphasis on genetically edited products that have reached the market in recent years.

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Cucumber Ribosomal Protein CsRPS21 Interacts With P22 Protein of Cucurbit Chlorotic Yellows Virus

Cited by 3 publications

References 43 publications

The interaction between the lemon ribosomal protein ClRPS9‐2 and citrus yellow vein clearing virus coat protein affects viral infection and gene silencing suppressor activity

The interaction between the lemon ribosomal protein ClRPS9‐2 and citrus yellow vein clearing virus coat protein affects viral infection and gene silencing suppressor activity

Synthesis, Anti-TMV Activities, and Action Mechanisms of a Novel Cytidine Peptide Compound

Tropical Fruit Virus Resistance in the Era of Next-Generation Plant Breeding

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