Cucumber Necrosis Virus Is a Member of the Tombusvirus Group

Abstract

SUMMARYThe electrophoretic patterns of dsRNA species isolated from cucumber plants infected with cucumber necrosis virus (CNV) and from Nicotiana clevelandii plants infected with tomato bushy stunt virus (TBSV) were almost identical and differed greatly from the electrophoretic patterns of dsRNA species isolated from tobacco plants infected with tobacco necrosis virus (TNV). Hybridization with cloned cDNA of CNV showed that there was extensive homology between the nucleic acids of CNV and TBSV but not between those of CNV and TNV. Gel diffusion tests and ELISAs showed no serological relationship between CNV and five tombusviruses. TBSVinfected N. clevelandii plants showed some cross-protection against infection by CNV but not against infection by carnation ringspot virus. CNV therefore resembles TBSV more than TNV and should be classified as a tombusvirus.

Translation of cucumber necrosis virus RNA in vitro

“…Cucumber necrosis virus (CNV), a member of the tombusvirus group (Rochon & Tremaine, 1988), is an isometric plant virus with a 30 nm particle composed of 180 copies of a single capsid protein with an approximate Mr of 41K (Tremaine, 1972). The CNV genome is monopartite and consists of single-stranded, positivesense RNA of approximately 4.7 kb (Tremaine, 1972;.…”

Encapsidation of Host RNAs by Cucumber Necrosis Virus Coat Protein during both Agroinfiltration and Infection

“…Cucumber necrosis virus (CNV) is a member of the Tombusvirus genus in the family Tombusviridae (27)(28)(29). As such, the genome is positive-sense, single-stranded RNA encapsidated by a Tϭ3 icosahedral shell.…”

Sequence and structure of defective interfering RNAs associated with cucumber necrosis virus infections

“…Cucumber necrosis tombusvirus (CNV) (Rochon & Tremaine, 1988) is an icosahedral virus with a monopartite genome of positive-sense ssRNA approximately 4.7 kb in length (Rochon & Tremaine, 1989). The only known natural systemic host for CNV is the cucumber; however, the experimental host range of CNV is wide and diversified (McKeen, 1959).…”