Translation of cucumber necrosis virus RNA in vitro

Johnston, James D.; Rochon, D’Ann

doi:10.1099/0022-1317-71-10-2233

Cited by 24 publications

(10 citation statements)

References 36 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These hybridization analyses illustrate that AMCV genome expression involves the production of two 3' coterminal sg RNAs of about 2.2 kb (sgl) and 0.9 kb (sg2). Analogous results were obtained for three other tombusviruses: CyRSV , TBSV (Hillman et al, 1989) and CNV (Johnston & Rochon, 1990). Furthermore, AMCV genomic RNA is encapsidated in the virus particles at a higher frequency than sg RNAs.…”

Section: Analysis Of Viral Rnassupporting

confidence: 64%

Nucleotide sequence, genomic organization and synthesis of infectious transcripts from a full-length clone of artichoke mottle crinkle virus

Tavazza

Lucioli

Calogero

et al. 1994

Journal of General Virology

View full text Add to dashboard Cite

The complete nucleotide sequence of the genome of artichoke mottle crinkle Virus (AMCV), a member of the tombusvirus group, has been determined. The genome is 4790 nucleotides (nt) in length. A full-length cDNA of the AMCV genome has been cloned in pUC9 downstream of the T7 RNA polymerase promoter. Transcripts were infective when inoculated onto Nicotiana clevelandii and N. benthamiana plants. The AMCV genome contains five open reading frames (ORFs). The first ORF from the 5' terminus (ORF1) encodes a protein with a predicted Mr of 33K. ORF2 extends through the amber termination codon of ORF1 to yield a polypeptide of predicted M r 92K and which is the putative RNAdependent RNA polymerase. ORF3 codes for the coat protein (41K). Two nested ORFs in different reading frames (ORFs 4 and 5) code for a 22K and a 19K polypeptide respectively. Sequence homologies suggest that the 22K protein could be involved in cell-to-cell movement of virus. ORFs 3, 4 and 5 are translated from two 3' coterminal subgenomic (sg) RNAs, the 5' termini of which have been mapped. The two sg RNAs are 2155 (sgl) and 934 (sg2) nt in length. ORF3 is expressed from sgl RNA whereas ORFs 4 and 5 are potentially expressed from sg2 RNA. Time course experiments with Cynara scolymus protoplasts indicate that during AMCV infection both positive and negative strands of genomic and sg RNAs are produced and that sg2 RNA is produced before and at a higher level than sgl RNA.

show abstract

Section: Analysis Of Viral Rnassupporting

confidence: 64%

Nucleotide sequence, genomic organization and synthesis of infectious transcripts from a full-length clone of artichoke mottle crinkle virus

Tavazza

Lucioli

Calogero

et al. 1994

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The p92 protein, which arises from readthrough of the amber terminator for the p33 open reading frame, contains the canonical Gly-Asp-Asp tripeptide and surrounding hydrophobic amino acids and thus is presumed to be at least part of the virus-encoded RNA-dependent RNA polymerase (18). Amino acid sequence comparisons and immunoprecipitation analyses have demonstrated that p41 is the coat protein (18,19). The functions of p33, p21, and p20 are not known.…”

mentioning

confidence: 99%

Rapid de novo generation of defective interfering RNA by cucumber necrosis virus mutants that do not express the 20-kDa nonstructural protein.

Rochon

1991

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

It is generally believed that serial passage at high multiplicity of infection (moi) is required for the generation of defective interfering (DI) particles. High levels of DI RNAs are found associated with persistent infections initiated with laboratory cultures of cucumber necrosis virus (CNV). Two synthetic CNV transcripts that were derived through site-directed mutagenesis of a highly infectious CNV cDNA clone and that do not express the CNV 20-kDa nonstructural protein were found to generate high levels of symptomattenuating DI RNAs de novo without serial high-moi passage in transcript-inoculated plants. Such de novo generation of DI RNAs did not occur in infections initiated with wild-type transcript until at least eight serial high-moi passages. The observation that a CNV nonstructural protein mutant rapidly generates DI RNA de novo may provide insight into mechanisms that underly DI particle formation in RNA viruses in general.Defective interfering (DI) particles are found in association with most animal viruses (1, 2) and appear in cell culture as a consequence of several serial passages of virus inoculum at high multiplicity of infection (moi). DI RNAs are deletion mutants of the helper virus, usually replicate at the expense ofthe helper virus in doubly infected cells, and require helper virus for their multiplication in host cells (for reviews see refs. 3-5). It has been suggested that DI particles may act as modulators of disease symptoms in animals; however, this has not been firmly established (6). In contrast to the ubiquity of DI particles in animal viruses, DI particles have only been well documented in two related groups of plant viruses, the tombusviruses and the carmoviruses (7-11). Recently, DI particles have also been found to be associated with two other plant virus groups, the geminiviruses (12) and the potexviruses (13). In the cherry strain of tomato bushy stunt virus (TBSV-Ch), a tombusvirus, DI RNAs were found to be colinear deletion mutants of genomic RNA that replicated to high levels in plants at the expense of virus accumulation. TBSV-Ch DI particles require the presence of the helper virus genome and are correlated with a dramatic attenuation of the severe necrosis typical of the helper virus alone in solanaceous hosts (7). In addition, TBSV-Ch DI RNA interferes with the replication of TBSV genomic RNA in protoplasts (14). More recently, it has been shown that TBSV-Ch DIs arise spontaneously upon serial high-moi passage in planta and that the de novo appearance ofDI RNAs is closely associated with attenuation of symptoms induced by the virus (15). De novo generation of DI RNA by synthetic RNA inocula derived from full-length cDNA clones of the carmovirus turnip crinkle virus (9) and a tombusvirus cymbidium ringspot virus (CyRSV) (16) has also been demonstrated.Cucumber necrosis virus (CNV), a biologically distinct tombusvirus (17), is an isometric virus containing a monopartite, positive-polarity RNA genome. The sequence of the CNV genome predicts the synthesis of five di...

show abstract

“…The CNV CP is translated from a subgenomic RNA (sgRNA1) during infection, as are p21 and p20 (sgRNA2). p33 is translated from full-length genomic RNA, and p92 is a readthrough product of the leaky amber termination codon of the p33 open reading frame (ORF) (29)(30)(31)(32)(33)). …”

mentioning

confidence: 99%

Encapsidation of Host RNAs by Cucumber Necrosis Virus Coat Protein during both Agroinfiltration and Infection

Ghoshal

Theilmann

Reade

et al. 2015

J Virol

View full text Add to dashboard Cite

show abstract

Translation of cucumber necrosis virus RNA in vitro

Cited by 24 publications

References 36 publications

Nucleotide sequence, genomic organization and synthesis of infectious transcripts from a full-length clone of artichoke mottle crinkle virus

Nucleotide sequence, genomic organization and synthesis of infectious transcripts from a full-length clone of artichoke mottle crinkle virus

Rapid de novo generation of defective interfering RNA by cucumber necrosis virus mutants that do not express the 20-kDa nonstructural protein.

Encapsidation of Host RNAs by Cucumber Necrosis Virus Coat Protein during both Agroinfiltration and Infection

Contact Info

Product

Resources

About