Cucumber mosaic virus-associated RNA 5 XI. Comparison of 14 CARNA 5 variants relates ability to induce tomato necrosis to a conserved nucleotide sequence

Kaperi, J.M.; Tousignant, M. E.; Steen, Mårten

doi:10.1016/0042-6822(88)90268-1

Cited by 49 publications

(22 citation statements)

References 33 publications

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“…However other necrogenic satellite RNAs do not have an ORF which extends through nucleotides 318 to 325 (Kaper et al, 1988). Analysis of their sequences shows that they are all identical around the positions implicated in necrogenesis (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The necrogenic domain identified above is within a short open reading frame (ORF liB; Kaper et al, 1988) (Fig. 5), but a frameshift mutation in ORF IIB had no detectable effect.…”

Section: Are Polypeptides Encoded By the Y Satellite Rna Necessary Fomentioning

confidence: 99%

“…Alignment of several satellite RNA sequences between position 259 (AsulI) and the 3' end. The sequences of the satellite RNAs have been previously described by Devic et al (1989) for Y and Ra, Jacquemond & Lauquin (1988) for I17N and R, Richards et al (1978) for n, Kaper et al (1988) :~ Viral symptoms were not produced on systemically infected leaves. § The progeny was approximately 2~ of the level of non-mutated satellite RNA in the control plant.…”

Section: Benign Bmentioning

confidence: 99%

“…The presence of the satellite RNA in a viral inoculum often has an effect on the symptoms induced by the helper virus (Francki, 1985). Cucumber mosaic virus (CMV) satellite RNAs have been extensively studied and, up to now, more than 25 isolates originating from widely separated geographical areas have been characterized and sequenced either directly from RNA or from cloned eDNA (Richards et al, 1978;Collmer et al, 1983 ;Gordon & Symons, 1983;Avila-Rincon et al, 1986;Kaper et al, 1986Kaper et al, , 1988Garcia-Arenal et al, 1987;Hidaka et al, 1988;Jacquemond & Lauquin, 1988;Devic et al, 1989;Masuta & Takanami, 1989). These isolates can be classified in two groups (benign or necrogenic) according to their ability to produce symptoms on tomato.…”

Section: Introductionmentioning

confidence: 99%

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Cucumber mosaic virus satellite RNA (strain Y): analysis of sequences which affect systemic necrosis on tomato

Devic

Jaegle²,

Baulcombe

1990

Journal of General Virology

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The location of a sequence within the Y satellite RNA of cucumber mosaic virus (CMV) that confers the ability to induce necrosis on tomato plants has been analysed using chimeric satellite RNAs. These recombinant RNA molecules contained parts of the Y (necrogenic) and Ra (benign) satellite RNAs and were inoculated into tomato plants together with CMV helper virus. From the composition of the recombinant satellite RNAs that induced necrosis it was concluded that, of the nucleotides which differ between Y and Ra satellite RNAs, those affecting necrosis are on the 3' side of nucleotide 259. The composition of satellite RNAs that failed to induce necrosis implies that at least some of the necrogenic positions are on the 3' side of nucleotide 311. The symptoms induced by mutated forms of Y and Ra satellite RNAs showed that nucleotide spacing between positions 322 and 323 and sequence identity at one or more of nucleotides 318, 323 or 325 affects the necrogenic potential of Y satellite RNA. The effect of a frameshifting mutation in Y satellite RNA and the location of the necrogenic sites relative to open reading frames in other satellite RNAs suggested that necrosis is not caused by polypeptides encoded in satellite RNA.

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Section: Discussionmentioning

confidence: 99%

“…The necrogenic domain identified above is within a short open reading frame (ORF liB; Kaper et al, 1988) (Fig. 5), but a frameshift mutation in ORF IIB had no detectable effect.…”

Section: Are Polypeptides Encoded By the Y Satellite Rna Necessary Fomentioning

confidence: 99%

Section: Benign Bmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cucumber mosaic virus satellite RNA (strain Y): analysis of sequences which affect systemic necrosis on tomato

Devic

Jaegle²,

Baulcombe

1990

Journal of General Virology

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“…In addition CMV encapsidates a satellite RNA with the designation CARNA 5 (CMV-associated RNA 5), which comprises a group of sequencerelated variants 334-386 nucleotides (nt) long [2,3]. Although CARNA 5 is essentially sequence-unrelated to the CMV genome, it depends on the farter's presence for replication [4,5].…”

Section: Introductionmentioning

confidence: 99%

Replication of cucumber mosaic virus satellite RNA in vitro by an RNA‐dependent RNA polymerase from virus‐infected tobacco

Kaper

Jaspars

1991

FEBS Letters

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An RNA-dependent RNA polymerase purified from tobacco infected with cucumber mosaic virus catalyzes the synthesis of (-) and (+) strands of the viral satellite RNA, CARNA 5. but fails to replicate the satellite R.NA of peanut stunt virus (PSV). The enzyme replicates the genomie RNAs of the three principal cucumoviruses CMV. PSV and tomato aspermy virus (TAV) with varying efficiencies. The specificity with which CMV RdRp replicates differen', sequence-unrelated RNA templates suggests that the site of their recognition requires secondary or higher level structural organization.

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Structural Analysis of a Necrogenic Strain of Cucumber Mosaic Cucumovirus Satellite RNAin Planta

Rodríguez-Alvarado

Roossinck

1997

Virology

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Structural studies of plant viral RNA molecules have been based on in vitro chemical and enzymatic modification. That approach, along with mutational analysis, has proven valuable in predicting structural models for some plant viruses such as tobacco mosaic tobamovirus and brome mosaic bromovirus. However, in planta conditions may be dramatically different from those found in vitro. In this study we analyzed the structure of cucumber mosaic cucumovirus satellite RNA (sat RNA) strain D4 in vivo and compared it to the structures found in vitro and in purified virions. Following a methodology developed to determine the structure of 18S rRNA within intact plant tissues, different patterns of adenosine and cytosine modification were found for D4-sat RNA molecules in vivo, in vitro, and in virions. This chemical probing procedure identifies adenosine and cytosine residues located in unpaired regions of the RNA molecules. Methylation data, a genetic algorithm in the STAR RNA folding program, and sequence alignment comparisons of 78 satellite CMV RNA sequences were used to identify several helical regions located at the 5' and 3' ends of the RNA molecule. Data from previous mutational and sequence comparison studies between satellite RNA strains inducing necrosis in tomato plants and those strains not inducing necrosis allowed us to identify one helix and two tetraloop regions correlating with the necrogenicity syndrome.

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Cucumber mosaic virus-associated RNA 5 XI. Comparison of 14 CARNA 5 variants relates ability to induce tomato necrosis to a conserved nucleotide sequence

Cited by 49 publications

References 33 publications

Cucumber mosaic virus satellite RNA (strain Y): analysis of sequences which affect systemic necrosis on tomato

Cucumber mosaic virus satellite RNA (strain Y): analysis of sequences which affect systemic necrosis on tomato

Replication of cucumber mosaic virus satellite RNA in vitro by an RNA‐dependent RNA polymerase from virus‐infected tobacco

Structural Analysis of a Necrogenic Strain of Cucumber Mosaic Cucumovirus Satellite RNAin Planta

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Cucumber mosaic virus-associated RNA 5 XI. Comparison of 14 CARNA 5 variants relates ability to induce tomato necrosis to a conserved nucleotide sequence

Cited by 49 publications

References 33 publications

Cucumber mosaic virus satellite RNA (strain Y): analysis of sequences which affect systemic necrosis on tomato

Cucumber mosaic virus satellite RNA (strain Y): analysis of sequences which affect systemic necrosis on tomato

Replication of cucumber mosaic virus satellite RNA in vitro by an RNA&#x2010;dependent RNA polymerase from virus&#x2010;infected tobacco

Structural Analysis of a Necrogenic Strain of Cucumber Mosaic Cucumovirus Satellite RNAin Planta

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Replication of cucumber mosaic virus satellite RNA in vitro by an RNA‐dependent RNA polymerase from virus‐infected tobacco