Cucumber

Pląder, Wojciech; Burza, W.; Malepszy, S.

doi:10.1007/978-3-540-36752-9_10

Cited by 11 publications

(8 citation statements)

References 68 publications

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“…Cucumber production is subject to infections by a wide range of pathogens including leaf fungal diseases, Fusarium wilt symptoms, nematode infestation, anthracnose, scab, leaf blotch, and virus diseases. However, it is difficult to improve the tolerance of cucumber to pathogen attack by conventional breeding owing to its narrow genetic base, with a genetic variability of only 3–8 % (Plader et al 2007). Although grafting of cucumber plants onto cucurbitaceous rootstocks is a common way to avoid soilborne diseases and nematodes (Oda 2008), this technique is labor-intensive.…”

Section: Introductionmentioning

confidence: 99%

Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks

Nanasato

Konagaya

Okuzaki

et al. 2012

Plant Biotechnol Rep

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An improved method for genetic transformation of cucumber (Cucumis sativus L. cv. Shinhokusei No. 1) was developed. Vacuum infiltration of cotyledonary explants with Agrobacterium suspension enhanced the efficiency of Agrobacterium infection in the proximal regions of explants. Co-cultivation on filter paper wicks suppressed necrosis of explants, leading to increased regeneration efficiency. Putative transgenic plants were screened by kanamycin resistance and green fluorescent protein (GFP) fluorescence, and integration of the transgene into the cucumber genome was confirmed by genomic polymerase chain reaction (PCR) and Southern blotting. These transgenic plants grew normally and T1 seeds were obtained from 7 lines. Finally, stable integration and transmission of the transgene in T1 generations were confirmed by GFP fluorescence and genomic PCR. The average transgenic efficiency for producing cucumbers with our method was 11.9 ± 3.5 %, which is among the highest values reported until date using kanamycin as a selective agent.Electronic supplementary materialThe online version of this article (doi:10.1007/s11816-012-0260-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks

Nanasato

Konagaya

Okuzaki

et al. 2012

Plant Biotechnol Rep

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“…

IntroductionCucumber, a plant belonging to the family Cucurbitaceae, is among the top ten vegetables produced globally (Plader et al, 2007). This species is believed to have been domesticated in India for 3000 years and in eastern Iran and China for probably 2000 years (Aydemir, 2009).

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confidence: 99%

Induction and development of anther-derived gametic embryos in cucumber (Cucumis sativus L.) by optimizing the macronutrient and agar concentrations in culture medium

Abdollahi

Najafi²,

Sarikhani³

et al. 2016

Turk J Biol

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Anther culture response of a cucumber cultivar (Beta Alpha) and three Iranian landraces of cucumber (Basmenj, Isfahani, and Korki) on MS culture media with an altered macronutrient strength (half, full, 1.5-fold, and double-strength) or various concentrations of agar (0, 3.5, 7, and 14 g L -1 ) was evaluated in two separate experiments. The effect of different concentrations of agar on the development of callus-derived embryos was also assessed. The androgenic potentials of various cucumbers were significantly (P < 0.01) influenced by the different macronutrient strengths and agar concentrations, although anther culture responses could vary according to the cucumber type. A doubled strength of macronutrients in culture medium resulted in the highest percentage of callogenesis (100%) in Isfahani cucumber, while use of full strength of macronutrients produced the highest percentage of embryogenic calli (83.33%) in the mentioned landrace compared to other tested forms. The maximum number of gametic embryos (1.26 and 1.23 embryos/anther) differentiated from Isfahani anther cultures on full-and half-strength MS medium, respectively. In the second experiment, the highest percentages of callogenesis were obtained in MS culture media solidified with 14 g L -1 and 7 g L -1 agar for Basmenj, with 3.5 g L -1 agar or liquid medium for Isfahani, with 7 g L -1 agar for Beta Alpha, and with 14 g L -1 agar for Korki. Culture media solidified with 7 g L -1 agar produced the highest percentage of embryogenic calli compared to other treatments. Anther culture of Korki cucumber on culture medium with 14 g L -1 agar produced the maximum number of embryos per anther compared to other cucumbers. Improved production of mature somatic embryos (torpedo and cotyledonary embryos) was achieved with higher agar concentrations (7 and 14 g L -1 ) for the cucumber cultivar and all landraces.

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“…The genome comprises 89 protein-coding genes, 8 rRNA genes and 37 tRNA genes. Some of these genes contain introns [ 29 ]. Our aim was to obtain a microarray probe set allowing for both expression studies of known plastid genes or conserved ORFs, as well as discovery of new RNAs transcribed from non-protein coding regions of the cucumber chloroplast genome.…”

Section: Resultsmentioning

confidence: 99%

A tiling microarray for global analysis of chloroplast genome expression in cucumber and other plants

et al. 2011

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Plastids are small organelles equipped with their own genomes (plastomes). Although these organelles are involved in numerous plant metabolic pathways, current knowledge about the transcriptional activity of plastomes is limited. To solve this problem, we constructed a plastid tiling microarray (PlasTi-microarray) consisting of 1629 oligonucleotide probes. The oligonucleotides were designed based on the cucumber chloroplast genomic sequence and targeted both strands of the plastome in a non-contiguous arrangement. Up to 4 specific probes were designed for each gene/exon, and the intergenic regions were covered regularly, with 70-nt intervals. We also developed a protocol for direct chemical labeling and hybridization of as little as 2 micrograms of chloroplast RNA. We used this protocol for profiling the expression of the cucumber chloroplast plastome on the PlasTi-microarray. Owing to the high sequence similarity of plant plastomes, the newly constructed microarray can be used to study plants other than cucumber. Comparative hybridization of chloroplast transcriptomes from cucumber, Arabidopsis, tomato and spinach showed that the PlasTi-microarray is highly versatile.

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Cucumber

Cited by 11 publications

References 68 publications

Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks

Improvement of Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.) by combination of vacuum infiltration and co-cultivation on filter paper wicks

Induction and development of anther-derived gametic embryos in cucumber (Cucumis sativus L.) by optimizing the macronutrient and agar concentrations in culture medium

A tiling microarray for global analysis of chloroplast genome expression in cucumber and other plants

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