Partial cubes are graphs isometrically embeddable into hypercubes. In this paper it is proved that every cubic, vertex-transitive partial cube is isomorphic to one of the following graphs: K 2 C 2n , for some n ≥ 2, the generalized Petersen graph G(10, 3), the cubic permutahedron, the truncated cuboctahedron, or the truncated icosidodecahedron. This classification is a generalization of results of Brešar et al. from 2004 on cubic mirror graphs, it includes all cubic, distance-regular partial cubes (Weichsel, 1992), and presents a contribution to the classification of all cubic partial cubes.