Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars

Kayıhan, Ceyhun; Eyidoğan, Füsun; Afsar, N.; Öktem, Hüseyin Avni; Yucel, Meral

doi:10.1007/s10535-012-0143-x

Cited by 32 publications

(23 citation statements)

References 32 publications

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“…Significant positive correlation (R = 0.61, p B 0.05) between the activity of this enzyme and ME effectiveness confirmed the important role played by this enzyme in ME induction. Increased SOD activity is usually the first response to ROS (particularly O ÁÀ 2 ) accumulation (Alscher et al 2002;Kayihan et al 2012) and has been reported in plants exposed to various environmental stresses, where it was often correlated with increased stress tolerance (reviewed by Sharma et al 2012). In all studied DH lines of triticale, both copper/zinc and manganese isoforms of SOD were detected and accumulated under LT pre-treatment, which suggests that ROS were produced in almost all cellular compartments including mitochondria, chloroplasts, peroxisomes, cytosol, and apoplast.…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Żur

Dubas

Krzewska

et al. 2014

Plant Cell Tiss Organ Cult

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To verify the hypothesis that cell redox status regulates the process of microspore embryogenesis (ME), reactive oxygen species (ROS) generation and the activity of enzymatic and non-enzymatic antioxidants were analyzed in eight doubled haploid lines of triticale with significant differences in embryogenic potential. The analyses were performed in anthers excised from freshly cut tillers (control) and from low temperature (LT) pre-treated tillers (3 weeks at 4°C) in which ME has been initiated. Significant associations between ME effectiveness and the variables studied were found. In control cultures, high superoxide dismutase (SOD) activity appeared crucial for microspore viability. On the other hand, positive though non-linear correlation between ME effectiveness and H 2 O 2 generation, and negative correlation with catalase (CAT) activity suggest that some threshold level of H 2 O 2 is important for successful ME initiation. LT tillers pretreatment significantly increased H 2 O 2 accumulation, which had a negative effect on ME effectiveness. However, even high level of H 2 O 2 did not endanger cell viability as long as the cells exhibited high activity of ROS-decomposing enzymes (SOD, CAT and POX). The ability to sustain antioxidative enzyme activity under cold stress in the dark was another important requirement for high effectiveness of ME, allowing for the generation of the signal initiating microspore reprogramming and simultaneously protecting the cells from the toxic effects of ROS production. The role of antioxidative enzymes cannot be replaced even by high activity of non-enzymatic antioxidants. In conclusion, genetically controlled but environmentally modified cell tolerance to oxidative stress seems to play an important role in triticale ME.

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Section: Discussionmentioning

confidence: 99%

“…The lack of FeSOD was not surprising, as despite extensive research, the absence of this isozym was often reported e.g. in rice, maize (Alscher et al 2002) or barley cultivars (Kayihan et al 2012). Similar to our study, new isoforms of SOD were not detected in cold acclimated wheat seedlings, either (Scebba et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Żur

Dubas

Krzewska

et al. 2014

Plant Cell Tiss Organ Cult

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“…In addition, under excess light, different reactive oxygen species (ROS) are produced, such as the singlet state oxygen ( 1 O2) and H2O2 (Apel and Hirt 2004, Takahashi and Murata 2006, Wang et al 2012, Campbell et al 2013. To alleviate this oxidative damage, plants use complex defense systems including the antioxidant enzymes, such as superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), and guaiacol peroxidase (GPX,EC 1.11.17), and enzymes in the ascorbate-glutathione (AsA-GSH) cycle, such as ascorbate peroxidase (APX, EC 1.11.1.11), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1), and glutathione reductase (GR, EC 1.6.4.2); these antioxidant enzymes participate in scavenging of ROS in plants (Asada 2000, Guo et al 2006, Kayihan et al 2012 .…”

Section: Introductionmentioning

confidence: 99%

Influence of shading on photosynthesis and antioxidative activities of enzymes in apple trees

Liu¹,

Zhang²,

Wang³

et al. 2019

Photosynt.

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This study investigated the effects of shading on photosynthesis in 3-year-old potted apple trees. The trees were grown either in full sunlight or in a shade environment with about 50% of sunlight filtered through black plastic nets. Under the shade conditions, the net photosynthetic rate (PN), the stomatal conductance, the transpiration rate, and the intercellular CO2 concentration decreased before midday, which was due to low light energy but the PN of the shaded trees increased relative to the control trees during the afternoon. In addition, the concentrations of chlorophyll (Chl) a, b, and total Chl increased in the shaded trees. In shaded apple trees, the photochemical efficiency increased. There was a lower content of reactive oxygen species and activities of antioxidant enzymes. The results showed that moderate shading can improve the total photosynthetic efficiency, which is mainly attributed to alleviation of photodamage and low accumulation of reactive oxygen species.

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“…Elevated cold tolerance can be accompanied by the increase in expression of specific genes encoding antioxidant enzymes (Baek and Skinner, 2003). Kayihan et al (2012) investigated the effects of cold acclimation and freezing on Cu/ Zn superoxide dismutase activity and respective gene expression in barley (Hordeum vulgare) cultivars and found that leaf Cu/ZnSOD expression levels were unchanged during cold acclimation but increased evidently at -3°C freezing stress. Seedling leaves of manioc (Jatropha curcas) subjected to chilling shock exhibited a higher level of SOD, APX, CAT, and glutathione reductase than the control (Ao et al, 2013).…”

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confidence: 99%

Antioxidant Responses and Gene Expression in Bermudagrass under Cold Stress

Fan¹,

Ren²,

Zhu³

et al. 2014

J. Amer. Soc. Hort. Sci.

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Cold stress is a key factor limiting resource use in bermudagrass (Cynodon dactylon). Under cold stress, bermudagrass growth is severely inhibited and the leaves undergo chlorosis. Therefore, rigorous investigation on the physiological and molecular mechanisms of cold stress in this turf species is urgent. The objective of this study was to investigate the physiological and molecular alteration in wild bermudagrass under cold stress, particularly the changes of transpiration rate, soluble sugar content, enzyme activities, and expression of antioxidant genes. Wild bermudagrass (C. dactylon) was planted in plastic pots (each 10 cm tall and 8 cm in diameter) filled with matrix (brown coal soil:sand 1:1) and treated with 4 °C in a growth chamber. The results displayed a dramatic decline in the growth and transpiration rates of the wild bermudagrass under 4 °C temperature. Simultaneously, cold severely destabilized the cell membrane as indicated by increased malondialdehyde content and electrolyte leakage value. Superoxide dismutase and peroxidase activities were higher in the cold regime than the control. The expression of antioxidant genes including MnSOD, Cu/ZnSOD, POD, and APX was vividly up-regulated after cold stress. In summary, our results contributed to the understanding of the role of the antioxidant system in bermudagrass’ response to cold.

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Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars

Cited by 32 publications

References 32 publications

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Antioxidant activity and ROS tolerance in triticale (×Triticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis

Influence of shading on photosynthesis and antioxidative activities of enzymes in apple trees

Antioxidant Responses and Gene Expression in Bermudagrass under Cold Stress

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