Cu,Zn- and Mn-Superoxide Dismutase Concentration in Human Colostrum and Mature Milk

Kiyosawa, Isao; Matuyama, Jun; Nyui, Shoko; Yoshida, Kayo

doi:10.1271/bbb.57.676

Cited by 11 publications

(12 citation statements)

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“…The concentration of SOD in bovine milk is approximately 100 times lower than in bovine blood (Hoolbrook and Hicks 1978). In human milk it is 2.0–2.3 times higher than in bovine milk (Kiyosawa et al. 1993).…”

Section: Enzymatic Antioxidants In Colostrummentioning

confidence: 99%

Antioxidants in Bovine Colostrum

Przybylska¹,

Albera²,

Kankofer³

2007

Reprod Domestic Animals

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Quality, content and properties of colostrum are crucial for the neonate and its further development. Due to essential differences between intrauterine and extrauterine environment, the neonate is exposed to oxidative stress conditions. Colostrum apart from nutrient and immunological components should contain antioxidative systems necessary for the protection against reactive oxygen species. This review describes available data on enzymatic and non-enzymatic antioxidants in colostrum. Due to the fact that the literature concerning bovine colostrum is scanty, the information based on bovine mature milk determinations as well as other species is provided. Bovine colostrum is used not only by calves, but also for the production of hyperimmunized colostrum, medicines or feed supplements. Quality of colostrum influences quality of mature milk. This is another reason, except from health of neonate, why antioxidative properties of bovine colostrum are of special importance and require further detailed elucidation.

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Section: Enzymatic Antioxidants In Colostrummentioning

confidence: 99%

Antioxidants in Bovine Colostrum

Przybylska¹,

Albera²,

Kankofer³

2007

Reprod Domestic Animals

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“…The enzyme in milk serum has the same specific activity, molecular weight and electrophoretic properties as bovine erythrocyte Cu/Zn-SOD (Hill, 1975;Asada, 1976;Korycka-Dahl et al 1979). In human milk the activity of Mn-SOD has also been identified (Kiyosawa et al 1993).…”

Section: Antioxidative Enzymes In Milkmentioning

confidence: 99%

Antioxidative factors in milk

2000

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Lipid auto-oxidation in milk is affected by a complex interplay of pro-and antioxidants. Several of these compounds are also important nutrients in the human diet and may have other physiological effects in the gastrointestinal tract and other tissues. Among antioxidative enzymes superoxide dismutase catalyses the dismutation of superoxide anion to hydrogen peroxide. The degradation of hydrogen peroxide can be catalysed by catalase and the selenoprotein glutathione peroxidase. The latter enzyme can also degrade lipid peroxides. Lactoferrin may have an important role by binding pro-oxidative iron ions. The occurrence of different forms of these antioxidative proteins in milk and available data on their functional role are reviewed. More remains to be learnt of individual compounds and as an example the potential role of seleno compounds in milk is virtually unknown. Antioxidative vitamins in milk can provide an important contribution to the daily dietary intake. Moreover vitamin E and carotenoids act as fat-soluble antioxidants, e.g. in the milk fat globule membrane, which is regarded as a major site of auto-oxidation. Vitamin C is an important water-soluble antioxidant and interacts in a complex manner with iron and fat-soluble antioxidants. The concentrations of these compounds in milk are affected by cow feeding rations and milk storage conditions. Since milk contains a number of antioxidants many reactions are possible and the specific function of each antioxidant cannot easily be defined. There are indications that other compounds may have antioxidative function and measurement of total antioxidative capacity should be a useful tool in evaluating their relative roles.

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“…Although Holbrook and Hicks (15) described a procedure for the isolation of SOD from milk, the purpose of their study was one of characterization rather than efficient exploitation of the technique for preparative purposes. To date, most work on preparative recovery of SOD has used blood as the source material, primarily owing to the several‐hundred‐fold higher concentration of the enzyme in this feedstock (10, 13, 16).…”

Section: Introductionmentioning

confidence: 99%

Demonstration of a Strategy for Product Purification by High-Gradient Magnetic Fishing: Recovery of Superoxide Dismutase from Unconditioned Whey

Meyer

Hansen

Gomes

et al. 2008

Biotechnol Progress

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A systematic approach for the design of a bioproduct recovery process employing magnetic supports and the technique of high-gradient magnetic fishing (HGMF) is described. The approach is illustrated for the separation of superoxide dismutase (SOD), an antioxidant protein present in low concentrations (ca. 0.15-0.6 mg L(-1)) in whey. The first part of the process design consisted of ligand screening in which metal chelate supports charged with copper(II) ions were found to be the most suitable. The second stage involved systematic and sequential optimization of conditions for the following steps: product adsorption, support washing, and product elution. Next, the capacity of a novel high-gradient magnetic separator (designed for biotechnological applications) for trapping and holding magnetic supports was determined. Finally, all of the above elements were assembled to deliver a HGMF process for the isolation of SOD from crude sweet whey, which consisted of (i) binding SOD using Cu2+ -charged magnetic metal chelator particles in a batch reactor with whey; (ii) recovery of the "SOD-loaded" supports by high-gradient magnetic separation (HGMS); (iii) washing out loosely bound and entrained proteins and solids; (iv) elution of the target protein; and (v) recovery of the eluted supports from the HGMF rig. Efficient recovery of SOD was demonstrated at approximately 50-fold increased scale (cf magnetic rack studies) in three separate HGMF experiments, and in the best of these (run 3) an SOD yield of >85% and purification factor of approximately 21 were obtained.

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Cu,Zn- and Mn-Superoxide Dismutase Concentration in Human Colostrum and Mature Milk

Cited by 11 publications

References 0 publications

Antioxidants in Bovine Colostrum

Antioxidants in Bovine Colostrum

Antioxidative factors in milk

Demonstration of a Strategy for Product Purification by High-Gradient Magnetic Fishing: Recovery of Superoxide Dismutase from Unconditioned Whey

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