CTX-M-27–Producing <i>Escherichia coli</i> of Sequence Type 131 and Clade C1-M27, France

Birgy, André; Bidet, Philippe; Lévy, Corinne; Sobral, Elsa; Cohen, Robert; Bonacorsi, Stéphane

doi:10.3201/eid2305.161865

Cited by 39 publications

(25 citation statements)

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“…Interestingly, our recent study showed that the C1-M27 clade accounted for 38.7% of Japanese ESBL-producing ST131 isolates and was the most prevalent ST131 clade among these isolates (24). It should be noted that this clade is also present among clinical ST131 isolates from Thailand, Australia, Canada, and the United States (14), and a recent study also showed the prevalence of the C1-M27 clade in the fecal carriage of children in France (25), indicating contribution of this clade to the global spread of ST131. Although our ESBLEC collection may not represent the actual clonal compositions of ESBLEC in wastewater, the prevalence of this clade among the collection is a concern.…”

Section: Resultsmentioning

confidence: 86%

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Gomi

Matsuda

Matsumura

et al. 2017

Antimicrob Agents Chemother

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Contamination of environmental waters by extended-spectrum-␤-lactamase (ESBL)-producing Escherichia coli (ESBLEC) is of great concern. Wastewater treatment plants (WWTPs) and hospitals release large amounts of ESBLEC into the environment. In the present study, we isolated ESBLEC strains from wastewater collected from a WWTP and a hospital in Japan and performed whole-genome sequencing to characterize these strains. Genomic analysis of 54 strains (32 from the WWTP and 22 from hospital wastewater) revealed the occurrence of clinically important clonal groups with extraintestinal pathogenic E. coli status in the WWTP and hospital wastewater. Fine-scale phylogenetic analysis was performed to further characterize 15 sequence type 131 (ST131) complex strains (11 from the WWTP and 4 from hospital wastewater). These ST131 complex strains were comprised of the following different subgroups: clade A (n ϭ 2), C1-M27 (n ϭ 8), and C1 (non-C1-M27) (n ϭ 1) for strains from the WWTP and clade A (n ϭ 2), C1-M27 (n ϭ 1), and C1 (non-C1-M27) (n ϭ 1) for strains from hospital wastewater. The results indicate that ESBLEC strains belonging to clinically important lineages, including the C1-M27 clade, may disseminate into the environment through wastewater, highlighting the need to monitor for antibiotic resistance in wastewater.

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Section: Resultsmentioning

confidence: 86%

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Gomi

Matsuda

Matsumura

et al. 2017

Antimicrob Agents Chemother

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“…C1/H30R and C2/H30-Rx, come from clade B/H22 that is classically cephalosporin and fluoroquinolone susceptible (8,9). Recently, Matsumura et al have described the emergence of a distinct cluster (C1-M27) within the C1 subclade (10), belonging to previously reported strains in Asia, in North America, and in Europe (2,4,11,12). These isolates carry the bla CTX-M-27 gene and possess a similar region to a prophage-like genomic island of E. coli PCN033 (10).…”

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confidence: 97%

“…Thus, in the French population, we have recently shown the increasing carriage of CTX-M-27-producing E. coli in infants from no CTX-M-27 in 2010 (n ϭ 399) and 2011 (n ϭ 258) to 2.55% (11/431) in 2015. Within the ST131 clonal group, this corresponds to an increase from no CTX-M-27 in 2010% to 65% in 2015 (4,11).…”

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confidence: 99%

Independent Host Factors and Bacterial Genetic Determinants of the Emergence and Dominance of Escherichia coli Sequence Type 131 CTX-M-27 in a Community Pediatric Cohort Study

Birgy

Lévy

Nicolas–Chanoine

et al. 2019

Antimicrob Agents Chemother

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The recent emergence and diffusion in the community of Escherichia coli isolates belonging to the multidrug-resistant and CTX-M-27-producing sequence type 131 (ST131) C1-M27 cluster makes this cluster potentially as epidemic as the worldwide E. coli ST131 subclade C2 composed of multidrug-resistant isolates producing CTX-M-15. Thirty-five extended-spectrum beta-lactamase (ESBL)-producing ST131 isolates were identified in a cohort of 1,885 French children over a 5-year period. They were sequenced to characterize the ST131 E. coli isolates producing CTX-M-27 recently emerging in France. ST131 isolates producing CTX-M-27 (n = 17), and particularly those belonging to the C1-M27 cluster (n = 14), carried many resistance-encoding genes and predominantly an F1:A2:B20 plasmid type. In multivariate analysis, having been hospitalized since birth (odds ratio [OR], 10.9; 95% confidence interval [CI], 2.4 to 48.8; P = 0.002) and being cared for in a day care center (OR, 9.4; 95% CI, 1.5 to 59.0; P = 0.017) were independent risk factors for ST131 CTX-M-27 fecal carriage compared with ESBL-producing non-ST131 isolates. No independent risk factor was found when comparing CTX-M-15 (n = 11)- and CTX-M-1/14 (n = 7)-producing ST131 isolates with ESBL-producing non-ST131 isolates or with non-ESBL-producing isolates. Several factors may contribute to the increase in fecal carriage of CTX-M-27-producing E. coli isolates, namely, resistance to multiple antibiotics, capacity of the CTX-M-27 enzyme to hydrolyze both cefotaxime and ceftazidime, carriage of a peculiar F-type plasmid, and/or capacity to colonize children who have been hospitalized since birth or who attend day care centers.

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“…An increase in C1/H30R ST131 isolates was initially reported among clinical isolates in Japan; most of those were identified as members of the recently defined clade C1-M27 (90% of C1/H30R) ( 3 ). More recently, a dramatic rise from 0% to 65% in the incidence of ST131 C1/H30R bla CTX-M-27 isolates in the fecal carriage of children in France during 2010–2015 was reported ( 4 ). In addition, ST131 isolates harboring bla CTX-M-27 have been reported sporadically from other countries ( 3 ).…”

mentioning

confidence: 99%

“…All ST131 isolates with bla CTX-M-27 were of serogroup O25b and harbored a fimH 30 allele, except for 1 isolate that was of serogroup O16 and carried a fim H41 allele. Recently, the M27PP1 prophage-like region was defined as a specific marker for C1-M27 clade ( 3 , 4 ). This region was present in 23 of 24 bla CTX-M-27 –harboring isolates.…”

mentioning

confidence: 99%

bla_CTX-M-27–EncodingEscherichia coliSequence Type 131 Lineage C1-M27 Clone in Clinical Isolates, Germany

Ghosh¹,

Doijad²,

Falgenhauer³

et al. 2017

Emerg. Infect. Dis.

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CTX-M-27–Producing Escherichia coli of Sequence Type 131 and Clade C1-M27, France

Cited by 39 publications

References 2 publications

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Independent Host Factors and Bacterial Genetic Determinants of the Emergence and Dominance of Escherichia coli Sequence Type 131 CTX-M-27 in a Community Pediatric Cohort Study

bla_CTX-M-27–EncodingEscherichia coliSequence Type 131 Lineage C1-M27 Clone in Clinical Isolates, Germany

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CTX-M-27–Producing Escherichia coli of Sequence Type 131 and Clade C1-M27, France

Cited by 39 publications

References 2 publications

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Independent Host Factors and Bacterial Genetic Determinants of the Emergence and Dominance of Escherichia coli Sequence Type 131 CTX-M-27 in a Community Pediatric Cohort Study

blaCTX-M-27–EncodingEscherichia coliSequence Type 131 Lineage C1-M27 Clone in Clinical Isolates, Germany

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bla_CTX-M-27–EncodingEscherichia coliSequence Type 131 Lineage C1-M27 Clone in Clinical Isolates, Germany