CTX-M-15-Producing
            <i>Shigella sonnei</i>
            Strain from a Czech Patient Who Traveled in Asia

Hrabák, Jaroslav; Empel, Joanna; Gniadkowski, Marek; Halbhuber, Z.; Rébl, Karel; Urbášková, P

doi:10.1128/jcm.00427-08

Cited by 18 publications

(8 citation statements)

References 15 publications

(13 reference statements)

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“…CTX-M genes in the Shigellae have been previously reported in Argentina, (CTX-M-2) [51], Korea (CTX-M-14) [52] and from a traveler returning from India (CTX-M-15) [53]. More recently, Nagano et al described a novel CTX-M-64 hybrid from a shigellosis patient infected with S. sonnei after returning to Japan from China [54].…”

Section: Discussionmentioning

confidence: 93%

The Sudden Dominance of blaCTX–M Harbouring Plasmids in Shigella spp. Circulating in Southern Vietnam

et al. 2010

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BackgroundPlasmid mediated antimicrobial resistance in the Enterobacteriaceae is a global problem. The rise of CTX-M class extended spectrum beta lactamases (ESBLs) has been well documented in industrialized countries. Vietnam is representative of a typical transitional middle income country where the spectrum of infectious diseases combined with the spread of drug resistance is shifting and bringing new healthcare challenges.MethodologyWe collected hospital admission data from the pediatric population attending the hospital for tropical diseases in Ho Chi Minh City with Shigella infections. Organisms were cultured from all enrolled patients and subjected to antimicrobial susceptibility testing. Those that were ESBL positive were subjected to further investigation. These investigations included PCR amplification for common ESBL genes, plasmid investigation, conjugation, microarray hybridization and DNA sequencing of a bla CTX–M encoding plasmid.Principal FindingsWe show that two different bla CTX-M genes are circulating in this bacterial population in this location. Sequence of one of the ESBL plasmids shows that rather than the gene being integrated into a preexisting MDR plasmid, the bla CTX-M gene is located on relatively simple conjugative plasmid. The sequenced plasmid (pEG356) carried the bla CTX-M-24 gene on an ISEcp1 element and demonstrated considerable sequence homology with other IncFI plasmids.SignificanceThe rapid dissemination, spread of antimicrobial resistance and changing population of Shigella spp. concurrent with economic growth are pertinent to many other countries undergoing similar development. Third generation cephalosporins are commonly used empiric antibiotics in Ho Chi Minh City. We recommend that these agents should not be considered for therapy of dysentery in this setting.

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Section: Discussionmentioning

confidence: 93%

The Sudden Dominance of blaCTX–M Harbouring Plasmids in Shigella spp. Circulating in Southern Vietnam

et al. 2010

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“…(11), quinolone-resistant Shigella spp. (21), trimethoprim-sulfamethoxazole-resistant E. coli (6,32), SHV-12-producing Salmonella enterica serovar Typhi (1), CTX-M-15-producing Shigella sonnei (14), and CTX-M-15-producing E. coli (9).…”

Section: Resultsmentioning

confidence: 99%

Molecular Characteristics of Travel-Related Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates from the Calgary Health Region

Pitout

Campbell

Church

et al. 2009

Antimicrob Agents Chemother

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Extended-spectrum-␤-lactamase (ESBL)-producing Escherichia coli has recently emerged as a major risk factor for community-acquired, travel-related infections in the Calgary Health Region. Molecular characterization was done on isolates associated with infections in returning travelers using isoelectric focusing, PCR, and sequencing for bla CTX-M s, bla TEM s, bla SHV s , bla OXA s, and plasmid-mediated quinolone resistance determinants. Genetic relatedness was determined with pulsed-field gel electrophoresis using XbaI and multilocus sequence typing (MLST). A total of 105 residents were identified; 6/105 (6%) presented with hospital-acquired infections, 9/105 (9%) with health care-associated community-onset infections, and 90/105 (86%) with community-acquired infections. Seventy-seven of 105 (73%) of the ESBL-producing E. coli isolates were positive for bla CTX-M genes; 55 (58%) produced CTX-M-15, 13 (14%) CTX-M-14, six (6%) CTX-M-24, one (1%) CTX-M-2, one (1%) CTX-M-3, and one (1%) CTX-M-27, while 10 (10%) produced TEM-52, three (3%) TEM-26, 11 (11%) SHV-2, and four (4%) produced SHV-12. Thirty-one (30%) of the ESBL-producing E. coli isolates were positive for aac(6)-Ib-cr, and one (1%) was positive for qnrS. The majority of the ESBL-producing isolates (n ‫؍‬ 95 [90%]) were recovered from urine samples, and 83 (87%) were resistant to ciprofloxacin. The isolation of CTX-M-15 producers belonging to clone ST131 was associated with travel to the Indian subcontinent (India, Pakistan), Africa, the Middle East, and Europe, while clonally unrelated strains of CTX-M-14 and -24 were associated with travel to Asia. Our study suggested that clone ST131 coproducing CTX-M-15, OXA-1, TEM-1, and AAC(6)-Ib-cr and clonally unrelated CTX-M-14 producers have emerged as important causes of community-acquired, travel-related infections.

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“…These observations suggest that these plasmids are evolving through replicon sequence divergence, mosaicism, and replicon cointegration and resolution processes (80). IncF plasmids carrying the bla CTX-M-15 gene are not exclusive to clone ST131, since they were identified in other E. coli sequence types (ST405, ST354, ST28, and ST695), in a Shigella sonnei strain isolated from a Czech patient, in Salmonella enterica serovar Enteritidis in the United Kingdom, and in a Klebsiella pneumoniae strain in Spain (25,30,50). The bla CTX-M-15 gene expression is driven by the ISEcp1 insertion sequence, which is also implicated by its mobilization from the Kluyvera genome (93).…”

Section: Plasmids Carrying Esbls In Enterobacteriaceae Of Human and Amentioning

confidence: 99%

Resistance Plasmid Families in Enterobacteriaceae

Carattoli

2009

Antimicrob Agents Chemother

1,091

860

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CTX-M-15-Producing Shigella sonnei Strain from a Czech Patient Who Traveled in Asia

Cited by 18 publications

References 15 publications

The Sudden Dominance of blaCTX–M Harbouring Plasmids in Shigella spp. Circulating in Southern Vietnam

The Sudden Dominance of blaCTX–M Harbouring Plasmids in Shigella spp. Circulating in Southern Vietnam

Molecular Characteristics of Travel-Related Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates from the Calgary Health Region

Resistance Plasmid Families in Enterobacteriaceae

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