CTX-M-14 β-lactamase-producing Citrobacter freundii isolated in Venezuela

Abstract

A clinical isolate of C. freundii with reduced susceptibility to extended-spectrum β-lactams from a woman with cystocele associated with recurrent urinary tract infection was analyzed. Susceptibility tests, double disk synergy tests (DDST) and enzymatic activity by the agar iodometric method suggested the presence of ESBLs. Conjugation experiments revealed the presence of a large conjugative plasmid (pLM07/20) with an exclusive FrepB replicon type (IncF/FIB). PCR analysis and sequencing confirmed the presence of the blaCTX-M-14 gene in the pLM07/20 from C. freundii.LM07/10. Although this is the first report of CTX-M-14 in Venezuela, we alert the medical community that future increase of these β-lactamases in our city could be due to dissemination of plasmids into bacterial populations.

Multidrug resistant Gram-negative bacilli from neonatal septicaemia at a tertiary care centre in North India: A phenotypic and genotypic study

“…The three C. freundii isolates were all MDR and carried CTX-M gene, a fi nding reported from other countries as well in recent years. [10] In conclusion, we have highlighted changes in the bacterial aetiology of neonatal septicaemia, with non-fermenters emerging as important pathogens. Overall, prevalence of -lactamases was low in our setting but closely linked to MDR status.…”

First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring bla CTX-M-14 in South America

“…The detection of bla CTX-M-14 in South America is a recent phenomenon (10,19,21): so far, there have been no reports from our continent about this ESBL in Salmonella spp., although Salmonella Enteritidis isolates of human origin bearing blaCTX-M-14 have already been reported, mainly in Spain, China, Japan, and Korea (11,14,17,27).…”

ISCR1 asociado con genes blaCTX-M-1 y blaCTX-M-2 en plásmidos IncN e IncFIIA aislados en Klebsiella pneumoniae de origen nosocomial en Mérida, Venezuela

“…El tamaño de los plásmidos se determinó por comparación visual con otros de peso molecular conocidos: pLM07/20 (150kb) y pKAM542 (87kb) (10,15).…”

“…Recientemente, Millán, et al (10), reportaron la presencia de un plásmido conjugable del grupo IncF/FIB en Citrobacter freundii productor de BLEE de tipo CTX-M-14. Sin embargo, en Venezuela los estudios relacionados con el contexto genético de los genes codificantes para BLEE, especialmente los referentes a CTX-M, son escasos.…”