The objective of present study determines the role of work characteristics on employee performance in banking sector of Hyderabad, Pakistan. The primary data is collected from top five banks as suggested Stated Bank of Pakistan. The middle level employees are requested to fill the voluntary filled the self-administrative printed questionnaire during working hours of bank. Total 220 printed questionnaires were distributed among only 190 found reliable for data analysis while data cleaning process and for the analysis Smart PLS 3 is used. Findings of this study revealed that the three independent variables including skills variety, task significance and autonomy have positive and significant impact on dependent variable employee performance in banking sector of Hyderabad, Pakistan. In addition, two independent variables such as task identity and feedback have positive and insignificant impact on dependent variable employee performance in banking sector of Hyderabad, Pakistan. However, the skills variety is found to more positive and significant impact on employee performance because higher beta value with respect to other independent variables. Based on authors knowledge this is the first study which conducted in this regard of work characteristics and employee performance in banking sector of Hyderabad, Pakistan. Moreover, banking sector Hyderabad, Pakistan should improve the feedback system in their respective banks as suggested by this study.