CSE1L silence inhibits the growth and metastasis in gastric cancer by repressing GPNMB via positively regulating transcription factor MITF

Li, Yijun; Yuan, Shanshan; Liu, Jiaming; Wang, Yu; Zhang, Yanting; Chen, Xiaolu; Si, Wangli

doi:10.1002/jcp.29107

Cited by 26 publications

(27 citation statements)

References 35 publications

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“…45 Li found that CSE1L acted as an oncogene in gastric cancer (GC), and knockdown of CSE1L suppressed GC cell proliferation and metastasis via inhibition of glycoprotein nonmetastatic melanoma protein B (GPNMB) expression. 46 In this study, analysis of GEO datasets (GSE110224 and GSE37364) and IHC analysis showed that CSE1L was upregulated in CRC. Spearman correlation analysis indicated that CSE1L was positively correlated with circ_0060745 and that CSE1L was inversely correlated with miR-4736.…”

Section: Discussionmentioning

confidence: 64%

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

Wang

Ren

et al. 2020

OTT

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Purpose: Recent studies have shown that noncoding RNAs (ncRNAs) play essential roles in the development of a number of cancers. Circular RNAs (circRNAs) have been shown to contribute to the progression of colorectal cancer (CRC). Methods: In this study, the expression levels of circular RNA 0060745 (circ_0060745), and microRNA 4736 (miR-4736) were measured using qRT-PCR. Kaplan-Meier survival analysis and receiver operating characteristic (ROC) analysis were used to evaluate the diagnostic value of circ_0060745. Transwell assay and cell counting kit-8 (CCK8) assay were used to determine the metastatic and proliferative capacity of CRC cells. The expression of chromosome segregation one like (CSE1L) was measured using Western blotting and immunohistochemistry (IHC). In addition, RNA pull-down assay and luciferase assay were performed to verify the targeted binding between miR-473,6 and circ_0060745, and between as miR-4736 and CSE1L. Results: We showed that circ_0060745 was upregulated in CRC, and was associated with unfavorable clinicopathological characteristics. We also showed that circ_0060745 acted as an oncogene and promoted CRC cell proliferation and metastasis. Circ_0060745 was primarily located in the cytoplasm. Furthermore, miR-4736 was downregulated in CRC, was a downstream target of circ_0060745, and mediated proliferation and metastasis. We showed that circ_0060745 sequestered miR-4736, which resulted in CRC cell proliferation and metastasis. Finally, we showed that CSE1L, a downstream target of miR-4736, was upregulated in CRC and mediated suppression of proliferation and metastasis in CRC. Conclusion: The results of this study showed that circ_0060745 promoted CRC cell proliferation and metastasis via modulation of miR-4736/CSE1L signaling. The Circ_0060745/miR-4736/CSE1L axis might be a novel target for the treatment of CRC.

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Section: Discussionmentioning

confidence: 64%

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

Wang

Ren

et al. 2020

OTT

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“…To date, although a number of predictive molecular markers (e.g., 1p/19q co-deletion and IDH1/2 mutations) have been identified as prognostic markers for glioma ( 33 – 35 ), more are urgently required, partly due to the great complexity of the molecular traits of glioma ( 36 ). GPNMB, a transmembrane glycoprotein, has been demonstrated to be involved in tumor progression ( 11 , 12 , 21 , 22 ), whereas its mechanistic effects on glioma progression and its prognostic role have not been thoroughly investigated. Therefore, the aim of the present study was to elucidate the prognostic role of GPNMB in glioma through data mining of publicly accessible datasets through validation by immunohistochemical staining of a tissue microarray.…”

Section: Discussionmentioning

confidence: 99%

“…GPNMB is not only expressed in normal tissues (e.g. skin, bone, urinary system and CNS tissues), but also abnormally expressed in pathological tissues such as glaucoma, colitis, liver injury and a variety of carcinoma tissues ( 9 ), such as breast ( 10 ), gastric ( 11 ) or pancreatic cancer ( 12 ). GPNMB is located on the cell membrane and intracellular organelles, such as melanosomes and lysosomes, and can also be secreted into the extracellular compartment ( 13 – 15 ).…”

Section: Introductionmentioning

confidence: 99%

“…In normal tissues, GPNMB modulates various physiological processes, such as melanosome maturation ( 14 ), intercellular adhesion between keratinocytes and melanocytes ( 16 ), osteoclast and osteoblast differentiation ( 15 , 17 , 18 ), and the regulation of immune responses ( 19 , 20 ). Regarding the role of GPNMB in cancer, a variety of studies have demonstrated pro-tumorigenic roles of GPNMB in breast ( 10 , 21 ), gastric ( 11 ), lung ( 22 ) and pancreatic cancer ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

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High expression of GPNMB indicates an unfavorable prognosis in glioma: Combination of data from the GEO and CGGA databases and validation in tissue microarray

Xiao

Zhang

et al. 2020

Oncol Lett

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Glycoprotein non-metastatic melanoma protein B (GPNMB), a transmembrane glycoprotein, has been reported to be involved in tumor progression, but its prognostic value for glioma and the mechanistic effects on glioma progression have not been clearly explored. The present study aimed to investigate the prognostic role of GPNMB in glioma and the potential mechanisms of how GPNMB mediates glioma progression. Differentially expressed genes between the four highest and four lowest GPNMB expression samples in the GSE53733 dataset were first determined. Gene ontology, Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis and Gene set enrichment analysis results demonstrated that the significantly enriched pathways in samples with high GPNMB expression compared with those with low GPNMB expression were associated with hypoxia, angiogenesis, migration and invasion. Pearson correlation analysis was conducted to investigate the correlations between GPNMB expression and the markers of hypoxia, angiogenesis, migration and invasion in GSE53733, which were further validated using another mRNA microarray dataset from the Chinese Glioma Genome Atlas (CGGA). In addition, using the CGGA dataset, high GPNMB expression was demonstrated to be significantly associated with advanced WHO grade and short survival time in patients with glioma. Of note, based on the immunohistochemical staining of the tissue microarrays, Kaplan-Meier analysis with the Renyi test and a Cox proportional hazards model were used to validate the unfavorable prognostic role of high GPNMB expression in glioma. In conclusion, high GPNMB expression may be associated with high tumor grade and unfavorable prognosis in glioma. GPNMB expression was demonstrated to correlate with the markers of hypoxia, angiogenesis, migration and invasion, which may be potential mechanisms through which GPNMB mediates glioma progression.

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“…Human chromosomal segregation 1‐like ( CSE1L ) gene functions as an important mediator in cell nucleus, and is involved in cell proliferation, migration, and apoptosis. Overexpression of CSE1L has been shown to be associated with tumor development and cancerogenesis 70 . In a preclinical study on the lncRNA growth arrest‐specific transcript 5 gene ( GAS5 ), GAS5 ‐derived snoRNAs expression was upregulated to a DNA damage response p53 ‐dependently manner.…”

Section: The Lncrnas Mode Of Actionmentioning

confidence: 99%

Mechanisms of long non‐coding RNA function in colorectal cancer tumorigenesis

Poursheikhani

Abbaszadegan

Kerachian

2020

Asia-Pac J Clncl Oncology

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Colorectal cancer (CRC) is one of the most common cancers globally. Although a variety of CRC screening methods have been developed, many patients are diagnosed at advanced stages of CRC with tumor invasion and distance metastasis. Several studies have suggested the long noncoding RNAs (lncRNAs) as one of the main contributors in CRC tumorigenesis, although the exact underlying mechanism of lncRNAs in CRC is still unknown. Numerous studies have indicated aberrant expression of lncRNAs in CRC through different modes of action such as cell proliferation, apoptosis, cell cycle, DNA repair response, drug‐resistance, migration, and metastasis. Furthermore, lncRNA polymorphisms can influence the risk of CRC development. Accordingly, lncRNAs can be served as promising diagnostic or prognostic biomarkers and also desired therapeutic targets affecting the outcome of patients with CRC. In this review, we summarized the updated and novel evidence that identifies different roles of lncRNAs in the tumorigenesis of CRC.

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CSE1L silence inhibits the growth and metastasis in gastric cancer by repressing GPNMB via positively regulating transcription factor MITF

Cited by 26 publications

References 35 publications

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

<p>Circular RNA 0060745, a Novel circRNA, Promotes Colorectal Cancer Cell Proliferation and Metastasis Through miR-4736 Sponging</p>

High expression of GPNMB indicates an unfavorable prognosis in glioma: Combination of data from the GEO and CGGA databases and validation in tissue microarray

Mechanisms of long non‐coding RNA function in colorectal cancer tumorigenesis

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