Crystallization and preliminary X‐ray data for peanut agglutinin

Gold nanoparticle-based multivalent carbohydrate probes: selective photoaffinity labeling of carbohydrate-binding proteins

“…These data may be due to the differences in the geometry of carbohydrate binding sites on the proteins. Both PNA and RCA are tetrameric proteins having a pair of two carbohydrate binding site facing the same direction with ∼6 nm apart, 22 potentially allowing two Lac ligands on a probe to bind at the same time and crosslink more efficiently. ECA, in contrast, is a dimeric protein with the carbohydrate binding site at the opposite faces, 23 which likely binds single Lac ligand on a probe thus crosslinking less efficiently.…”